Echocardiographic Assessment of Embryonic and Fetal Mouse Heart Development: A Focus on Haemodynamics and Morphology
Background. Heart development is a complex process, and abnormal development may result in congenital heart disease (CHD). Currently, studies on animal models mainly focus on cardiac morphology and the availability of hemodynamic data, especially of the right heart half, is limited. Here we aimed to...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/531324 |
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| author | Nathan D. Hahurij Emmeline E. Calkoen Monique R. M. Jongbloed Arno A. W. Roest Adriana C. Gittenberger-de Groot Robert E. Poelmann Marco C. De Ruiter Conny J. van Munsteren Paul Steendijk Nico A. Blom |
| author_facet | Nathan D. Hahurij Emmeline E. Calkoen Monique R. M. Jongbloed Arno A. W. Roest Adriana C. Gittenberger-de Groot Robert E. Poelmann Marco C. De Ruiter Conny J. van Munsteren Paul Steendijk Nico A. Blom |
| author_sort | Nathan D. Hahurij |
| collection | DOAJ |
| description | Background. Heart development is a complex process, and abnormal development may result in congenital heart disease (CHD). Currently, studies on animal models mainly focus on cardiac morphology and the availability of hemodynamic data, especially of the right heart half, is limited. Here we aimed to assess the morphological and hemodynamic parameters of normal developing mouse embryos/fetuses by using a high-frequency ultrasound system. Methods. A timed breeding program was initiated with a WT mouse line (Swiss/129Sv background). All recordings were performed transabdominally, in isoflurane sedated pregnant mice, in hearts of sequential developmental stages: 12.5, 14.5, and 17.5 days after conception (n=105). Results. Along development the heart rate increased significantly from 125 ± 9.5 to 219 ± 8.3 beats per minute. Reliable flow measurements could be performed across the developing mitral and tricuspid valves and outflow tract. M-mode measurements could be obtained of all cardiac compartments. An overall increase of cardiac systolic and diastolic function with embryonic/fetal development was observed. Conclusion. High-frequency echocardiography is a promising and useful imaging modality for structural and hemodynamic analysis of embryonic/fetal mouse hearts. |
| format | Article |
| id | doaj-art-9e8854834db04c8c97bc7f4c55cbd11d |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-9e8854834db04c8c97bc7f4c55cbd11d2025-08-20T03:38:42ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/531324531324Echocardiographic Assessment of Embryonic and Fetal Mouse Heart Development: A Focus on Haemodynamics and MorphologyNathan D. Hahurij0Emmeline E. Calkoen1Monique R. M. Jongbloed2Arno A. W. Roest3Adriana C. Gittenberger-de Groot4Robert E. Poelmann5Marco C. De Ruiter6Conny J. van Munsteren7Paul Steendijk8Nico A. Blom9Department of Pediatric Cardiology, Leiden University Medical Center (LUMC), Leiden, P.O. Box 9600, 2300 RC, Leiden, The NetherlandsDepartment of Pediatric Cardiology, Leiden University Medical Center (LUMC), Leiden, P.O. Box 9600, 2300 RC, Leiden, The NetherlandsDepartment of Anatomy & Embryology, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The NetherlandsDepartment of Pediatric Cardiology, Leiden University Medical Center (LUMC), Leiden, P.O. Box 9600, 2300 RC, Leiden, The NetherlandsDepartment of Anatomy & Embryology, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The NetherlandsDepartment of Anatomy & Embryology, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The NetherlandsDepartment of Anatomy & Embryology, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The NetherlandsDepartment of Anatomy & Embryology, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The NetherlandsDepartment of Cardiology, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The NetherlandsDepartment of Pediatric Cardiology, Leiden University Medical Center (LUMC), Leiden, P.O. Box 9600, 2300 RC, Leiden, The NetherlandsBackground. Heart development is a complex process, and abnormal development may result in congenital heart disease (CHD). Currently, studies on animal models mainly focus on cardiac morphology and the availability of hemodynamic data, especially of the right heart half, is limited. Here we aimed to assess the morphological and hemodynamic parameters of normal developing mouse embryos/fetuses by using a high-frequency ultrasound system. Methods. A timed breeding program was initiated with a WT mouse line (Swiss/129Sv background). All recordings were performed transabdominally, in isoflurane sedated pregnant mice, in hearts of sequential developmental stages: 12.5, 14.5, and 17.5 days after conception (n=105). Results. Along development the heart rate increased significantly from 125 ± 9.5 to 219 ± 8.3 beats per minute. Reliable flow measurements could be performed across the developing mitral and tricuspid valves and outflow tract. M-mode measurements could be obtained of all cardiac compartments. An overall increase of cardiac systolic and diastolic function with embryonic/fetal development was observed. Conclusion. High-frequency echocardiography is a promising and useful imaging modality for structural and hemodynamic analysis of embryonic/fetal mouse hearts.http://dx.doi.org/10.1155/2014/531324 |
| spellingShingle | Nathan D. Hahurij Emmeline E. Calkoen Monique R. M. Jongbloed Arno A. W. Roest Adriana C. Gittenberger-de Groot Robert E. Poelmann Marco C. De Ruiter Conny J. van Munsteren Paul Steendijk Nico A. Blom Echocardiographic Assessment of Embryonic and Fetal Mouse Heart Development: A Focus on Haemodynamics and Morphology The Scientific World Journal |
| title | Echocardiographic Assessment of Embryonic and Fetal Mouse Heart Development: A Focus on Haemodynamics and Morphology |
| title_full | Echocardiographic Assessment of Embryonic and Fetal Mouse Heart Development: A Focus on Haemodynamics and Morphology |
| title_fullStr | Echocardiographic Assessment of Embryonic and Fetal Mouse Heart Development: A Focus on Haemodynamics and Morphology |
| title_full_unstemmed | Echocardiographic Assessment of Embryonic and Fetal Mouse Heart Development: A Focus on Haemodynamics and Morphology |
| title_short | Echocardiographic Assessment of Embryonic and Fetal Mouse Heart Development: A Focus on Haemodynamics and Morphology |
| title_sort | echocardiographic assessment of embryonic and fetal mouse heart development a focus on haemodynamics and morphology |
| url | http://dx.doi.org/10.1155/2014/531324 |
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