Association of expression of inflammatory response genes and DNA repair genes in colorectal carcinoma
Inflammation is an important etiological factor of colorectal carcinoma and may be related to colorectal carcinoma growth and proliferation. This study aimed to verify whether the presence of chronic inflammation represented by tumor necrosis factor-α, interleukin-2, interleukin-6, and interleukin-1...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2019-04-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428319843042 |
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| author | Demétrius Eduardo Germini Maria Isete Fares Franco Fernando Luiz Affonso Fonseca Flávia de Sousa Gehrke Beatriz da Costa Aguiar Alves Reis Leonardo Cardili Celina Tizuko Fujiyama Oshima Thérèse Rachell Theodoro Jaques Waisberg |
| author_facet | Demétrius Eduardo Germini Maria Isete Fares Franco Fernando Luiz Affonso Fonseca Flávia de Sousa Gehrke Beatriz da Costa Aguiar Alves Reis Leonardo Cardili Celina Tizuko Fujiyama Oshima Thérèse Rachell Theodoro Jaques Waisberg |
| author_sort | Demétrius Eduardo Germini |
| collection | DOAJ |
| description | Inflammation is an important etiological factor of colorectal carcinoma and may be related to colorectal carcinoma growth and proliferation. This study aimed to verify whether the presence of chronic inflammation represented by tumor necrosis factor-α, interleukin-2, interleukin-6, and interleukin-10 gene expression is related to hMLH1, hMSH2, hMSH6, and PMS2 gene expression and the corresponding protein levels of these genes from the DNA repair system. A total of 83 patients were operated on for curative or palliative colorectal carcinoma. Expression of the inflammatory response genes tumor necrosis factor-α, interleukin-2, interleukin-6, and interleukin-10 as well as expression of the hMLH1, hMSH2, hMSH6, and PMS2 genes of the DNA repair system (mismatch repair) and the expression levels of the corresponding mismatch repair proteins were measured in neoplastic tissue by reverse transcription polymerase chain reaction and immunohistochemistry, respectively. Associations were observed between hMSH6 mRNA expression and interleukin-2 mRNA expression (p = 0.026) as well as between hMLH1 and hMSH2 gene expression and tumor necrosis factor-α gene expression (p = 0.042). Higher tissue levels of interleukin-2 and tumor necrosis factor-α gene expression were associated with lower hMSH6, hMLH1, and hMSH2 gene expression. |
| format | Article |
| id | doaj-art-9e84c2b3f19a4860b6c6864a86da60aa |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2019-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-9e84c2b3f19a4860b6c6864a86da60aa2025-08-20T03:57:03ZengSAGE PublishingTumor Biology1423-03802019-04-014110.1177/1010428319843042Association of expression of inflammatory response genes and DNA repair genes in colorectal carcinomaDemétrius Eduardo Germini0Maria Isete Fares Franco1Fernando Luiz Affonso Fonseca2Flávia de Sousa Gehrke3Beatriz da Costa Aguiar Alves Reis4Leonardo Cardili5Celina Tizuko Fujiyama Oshima6Thérèse Rachell Theodoro7Jaques Waisberg8Department of Surgery, São Paulo State Civil Servant Hospital, São Paulo, BrazilDepartment of Pathology, São Paulo State Civil Servant Hospital, São Paulo, BrazilDepartment of Pathology, ABC School of Medicine, Santo André, BrazilDepartment of Pathology, ABC School of Medicine, Santo André, BrazilDepartment of Pathology, ABC School of Medicine, Santo André, BrazilDepartment of Pathology, Federal University of São Paulo, São Paulo, BrazilDepartment of Pathology, Federal University of São Paulo, São Paulo, BrazilDepartment of Morphology and Physiology, ABC School of Medicine, Santo André, BrazilDepartment of Surgery, ABC School of Medicine, Santo André, BrazilInflammation is an important etiological factor of colorectal carcinoma and may be related to colorectal carcinoma growth and proliferation. This study aimed to verify whether the presence of chronic inflammation represented by tumor necrosis factor-α, interleukin-2, interleukin-6, and interleukin-10 gene expression is related to hMLH1, hMSH2, hMSH6, and PMS2 gene expression and the corresponding protein levels of these genes from the DNA repair system. A total of 83 patients were operated on for curative or palliative colorectal carcinoma. Expression of the inflammatory response genes tumor necrosis factor-α, interleukin-2, interleukin-6, and interleukin-10 as well as expression of the hMLH1, hMSH2, hMSH6, and PMS2 genes of the DNA repair system (mismatch repair) and the expression levels of the corresponding mismatch repair proteins were measured in neoplastic tissue by reverse transcription polymerase chain reaction and immunohistochemistry, respectively. Associations were observed between hMSH6 mRNA expression and interleukin-2 mRNA expression (p = 0.026) as well as between hMLH1 and hMSH2 gene expression and tumor necrosis factor-α gene expression (p = 0.042). Higher tissue levels of interleukin-2 and tumor necrosis factor-α gene expression were associated with lower hMSH6, hMLH1, and hMSH2 gene expression.https://doi.org/10.1177/1010428319843042 |
| spellingShingle | Demétrius Eduardo Germini Maria Isete Fares Franco Fernando Luiz Affonso Fonseca Flávia de Sousa Gehrke Beatriz da Costa Aguiar Alves Reis Leonardo Cardili Celina Tizuko Fujiyama Oshima Thérèse Rachell Theodoro Jaques Waisberg Association of expression of inflammatory response genes and DNA repair genes in colorectal carcinoma Tumor Biology |
| title | Association of expression of inflammatory response genes and DNA repair genes in colorectal carcinoma |
| title_full | Association of expression of inflammatory response genes and DNA repair genes in colorectal carcinoma |
| title_fullStr | Association of expression of inflammatory response genes and DNA repair genes in colorectal carcinoma |
| title_full_unstemmed | Association of expression of inflammatory response genes and DNA repair genes in colorectal carcinoma |
| title_short | Association of expression of inflammatory response genes and DNA repair genes in colorectal carcinoma |
| title_sort | association of expression of inflammatory response genes and dna repair genes in colorectal carcinoma |
| url | https://doi.org/10.1177/1010428319843042 |
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