Effects of iron saturation on antibacterial activity of lactoferrin
The purpose of this study was to elucidate how iron-depleted and iron-saturated forms of lactoferrin (Lf) exert its antibacterial activity in vitro. Bacteria were divided into three groups by treated with different forms of porcine Lf: the control group (without addition of Lf), apolactoferrin (Apo-...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2022-06-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.06.211 |
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| author | WANG Zhenjie ZHANG Kang LIANG Li XIONG Qingqing DU Huahua |
| author_facet | WANG Zhenjie ZHANG Kang LIANG Li XIONG Qingqing DU Huahua |
| author_sort | WANG Zhenjie |
| collection | DOAJ |
| description | The purpose of this study was to elucidate how iron-depleted and iron-saturated forms of lactoferrin (Lf) exert its antibacterial activity in vitro. Bacteria were divided into three groups by treated with different forms of porcine Lf: the control group (without addition of Lf), apolactoferrin (Apo-Lf, iron saturation is 6.9%) group and hololactoferrin (Holo-Lf, iron saturation is 100.0%) group. The results showed that: 1) Native Lf and Apo-Lf significantly inhibited the growth of Escherichia coli K88 and Staphylococcus aureus (P＜0.01), which was not observed in Holo-Lf group. 2) Apo-Lf exerted antibacterial effect by chelating iron, and iron supplementation could eliminate the inhibitory effect of Apo-Lf on E. coli K88. 3) Scanning electron microscope (SEM) results revealed that Apo-Lf damaged the surface membranes of E. coli K88 and S. Aureus. However, Holo-Lf showed no effect on all of tested bacteria. 4) Predicted three-dimensional structures showed that the structures of Apo-Lf and Holo-Lf were markedly different, and the active site of Apo-Lf was more likely to directly interact with bacteria. Taken together, Apo-Lf inhibited the growth of E. coli K88 and S. Aureus by chelating iron or destroying the surface of bacteria. |
| format | Article |
| id | doaj-art-9e835dcdb9a34da5bb8c3e8a035e3c83 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-9e835dcdb9a34da5bb8c3e8a035e3c832025-08-20T03:16:11ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552022-06-014837738210.3785/j.issn.1008-9209.2021.06.21110089209Effects of iron saturation on antibacterial activity of lactoferrinWANG ZhenjieZHANG KangLIANG LiXIONG QingqingDU HuahuaThe purpose of this study was to elucidate how iron-depleted and iron-saturated forms of lactoferrin (Lf) exert its antibacterial activity in vitro. Bacteria were divided into three groups by treated with different forms of porcine Lf: the control group (without addition of Lf), apolactoferrin (Apo-Lf, iron saturation is 6.9%) group and hololactoferrin (Holo-Lf, iron saturation is 100.0%) group. The results showed that: 1) Native Lf and Apo-Lf significantly inhibited the growth of Escherichia coli K88 and Staphylococcus aureus (P＜0.01), which was not observed in Holo-Lf group. 2) Apo-Lf exerted antibacterial effect by chelating iron, and iron supplementation could eliminate the inhibitory effect of Apo-Lf on E. coli K88. 3) Scanning electron microscope (SEM) results revealed that Apo-Lf damaged the surface membranes of E. coli K88 and S. Aureus. However, Holo-Lf showed no effect on all of tested bacteria. 4) Predicted three-dimensional structures showed that the structures of Apo-Lf and Holo-Lf were markedly different, and the active site of Apo-Lf was more likely to directly interact with bacteria. Taken together, Apo-Lf inhibited the growth of E. coli K88 and S. Aureus by chelating iron or destroying the surface of bacteria.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.06.211lactoferrinantibacterialiron saturationapolactoferrinhololactoferrin |
| spellingShingle | WANG Zhenjie ZHANG Kang LIANG Li XIONG Qingqing DU Huahua Effects of iron saturation on antibacterial activity of lactoferrin 浙江大学学报. 农业与生命科学版 lactoferrin antibacterial iron saturation apolactoferrin hololactoferrin |
| title | Effects of iron saturation on antibacterial activity of lactoferrin |
| title_full | Effects of iron saturation on antibacterial activity of lactoferrin |
| title_fullStr | Effects of iron saturation on antibacterial activity of lactoferrin |
| title_full_unstemmed | Effects of iron saturation on antibacterial activity of lactoferrin |
| title_short | Effects of iron saturation on antibacterial activity of lactoferrin |
| title_sort | effects of iron saturation on antibacterial activity of lactoferrin |
| topic | lactoferrin antibacterial iron saturation apolactoferrin hololactoferrin |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.06.211 |
| work_keys_str_mv | AT wangzhenjie effectsofironsaturationonantibacterialactivityoflactoferrin AT zhangkang effectsofironsaturationonantibacterialactivityoflactoferrin AT liangli effectsofironsaturationonantibacterialactivityoflactoferrin AT xiongqingqing effectsofironsaturationonantibacterialactivityoflactoferrin AT duhuahua effectsofironsaturationonantibacterialactivityoflactoferrin |