Recent Findings in N6‐Methyladenosine Modification and Significance in Pancreatic Cancer

ABSTRACT Background RNA modifications are widely detected in cells and are involved in RNA structural stabilization and regulation of gene expression. In cancer cells, RNA modifications are altered, resulting in abnormal expression of numerous genes and promoting cancer growth. N1‐methyladenosine (m...

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Main Authors: Tomoaki Hara, Sikun Meng, Yasuko Arao, Yoshiko Saito, Kana Inoue, Sarah Rennie, Ken Ofusa, Toru Kitagawa, Hideshi Ishii
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:Cancer Medicine
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Online Access:https://doi.org/10.1002/cam4.70934
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author Tomoaki Hara
Sikun Meng
Yasuko Arao
Yoshiko Saito
Kana Inoue
Sarah Rennie
Ken Ofusa
Toru Kitagawa
Hideshi Ishii
author_facet Tomoaki Hara
Sikun Meng
Yasuko Arao
Yoshiko Saito
Kana Inoue
Sarah Rennie
Ken Ofusa
Toru Kitagawa
Hideshi Ishii
author_sort Tomoaki Hara
collection DOAJ
description ABSTRACT Background RNA modifications are widely detected in cells and are involved in RNA structural stabilization and regulation of gene expression. In cancer cells, RNA modifications are altered, resulting in abnormal expression of numerous genes and promoting cancer growth. N1‐methyladenosine (m1A), N6‐methyladenosine (m6A), N3‐methylcytosine (m3C), 5‐methylcytosine (m5C), 7‐methylguanosine (m7G), and N4‐acetylcytidine (ac4C) have been reported as RNA modifications affecting gene expression. Aim In this review, the function of m6A in pancreatic cancer is mainly described, and the current status and prospects of RNA modifications are discussed. Methodology We summarize recent reports on m6A writers METTL3, METTL5, METTL14, and METTL16; m6A readers IGF2BP1, IGF2BP2, IGF2BP3, YTHDF1, YTHDF2, and YTHDF3; and m6A erasers ALKBH5 and FTO. Results RNA modifications are written to the RNA by the writer, and the reader binds to the RNA modification, causing gene expression to increase or decrease. Gene expression is also regulated by the removal of RNA modifications by the eraser. Moreover, our recent investigation into m6A modifications in pancreatic cancer has led to the identification of several promising candidate biomarkers, highlighting the potential role of epitranscriptomic regulation in tumorigenesis. Conclusion These findings suggest that further exploration of RNA modification functions may facilitate the identification of novel biomarker and therapeutic target molecules for pancreatic cancer.
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spelling doaj-art-9e82441e5cec4aae8ca3d6424f9655052025-08-20T03:21:19ZengWileyCancer Medicine2045-76342025-06-011411n/an/a10.1002/cam4.70934Recent Findings in N6‐Methyladenosine Modification and Significance in Pancreatic CancerTomoaki Hara0Sikun Meng1Yasuko Arao2Yoshiko Saito3Kana Inoue4Sarah Rennie5Ken Ofusa6Toru Kitagawa7Hideshi Ishii8Department of Medical Data Science, Center of Medical Innovation and Translational Research Osaka University Graduate School of Medicine Osaka JapanDepartment of Medical Data Science, Center of Medical Innovation and Translational Research Osaka University Graduate School of Medicine Osaka JapanDepartment of Medical Data Science, Center of Medical Innovation and Translational Research Osaka University Graduate School of Medicine Osaka JapanDepartment of Medical Data Science, Center of Medical Innovation and Translational Research Osaka University Graduate School of Medicine Osaka JapanDepartment of Medical Data Science, Center of Medical Innovation and Translational Research Osaka University Graduate School of Medicine Osaka JapanSection for Computational and RNA Biology, Department of Biology University of Copenhagen Copenhagen DenmarkDepartment of Medical Data Science, Center of Medical Innovation and Translational Research Osaka University Graduate School of Medicine Osaka JapanDepartment of Medical Data Science, Center of Medical Innovation and Translational Research Osaka University Graduate School of Medicine Osaka JapanDepartment of Medical Data Science, Center of Medical Innovation and Translational Research Osaka University Graduate School of Medicine Osaka JapanABSTRACT Background RNA modifications are widely detected in cells and are involved in RNA structural stabilization and regulation of gene expression. In cancer cells, RNA modifications are altered, resulting in abnormal expression of numerous genes and promoting cancer growth. N1‐methyladenosine (m1A), N6‐methyladenosine (m6A), N3‐methylcytosine (m3C), 5‐methylcytosine (m5C), 7‐methylguanosine (m7G), and N4‐acetylcytidine (ac4C) have been reported as RNA modifications affecting gene expression. Aim In this review, the function of m6A in pancreatic cancer is mainly described, and the current status and prospects of RNA modifications are discussed. Methodology We summarize recent reports on m6A writers METTL3, METTL5, METTL14, and METTL16; m6A readers IGF2BP1, IGF2BP2, IGF2BP3, YTHDF1, YTHDF2, and YTHDF3; and m6A erasers ALKBH5 and FTO. Results RNA modifications are written to the RNA by the writer, and the reader binds to the RNA modification, causing gene expression to increase or decrease. Gene expression is also regulated by the removal of RNA modifications by the eraser. Moreover, our recent investigation into m6A modifications in pancreatic cancer has led to the identification of several promising candidate biomarkers, highlighting the potential role of epitranscriptomic regulation in tumorigenesis. Conclusion These findings suggest that further exploration of RNA modification functions may facilitate the identification of novel biomarker and therapeutic target molecules for pancreatic cancer.https://doi.org/10.1002/cam4.70934m6AMETTL3pancreatic cancerRNA modificationsTCEAL8
spellingShingle Tomoaki Hara
Sikun Meng
Yasuko Arao
Yoshiko Saito
Kana Inoue
Sarah Rennie
Ken Ofusa
Toru Kitagawa
Hideshi Ishii
Recent Findings in N6‐Methyladenosine Modification and Significance in Pancreatic Cancer
Cancer Medicine
m6A
METTL3
pancreatic cancer
RNA modifications
TCEAL8
title Recent Findings in N6‐Methyladenosine Modification and Significance in Pancreatic Cancer
title_full Recent Findings in N6‐Methyladenosine Modification and Significance in Pancreatic Cancer
title_fullStr Recent Findings in N6‐Methyladenosine Modification and Significance in Pancreatic Cancer
title_full_unstemmed Recent Findings in N6‐Methyladenosine Modification and Significance in Pancreatic Cancer
title_short Recent Findings in N6‐Methyladenosine Modification and Significance in Pancreatic Cancer
title_sort recent findings in n6 methyladenosine modification and significance in pancreatic cancer
topic m6A
METTL3
pancreatic cancer
RNA modifications
TCEAL8
url https://doi.org/10.1002/cam4.70934
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