Calcitonin Gene-Related Peptide Downregulates Expression of Inducible Nitride Oxide Synthase and Caspase-3 after Intestinal Ischemia-Reperfusion Injury in Rats
Various investigations have demonstrated that calcitonin gene-related peptide (CGRP) plays an important role in mediating ischemic preconditioning. CGRP has been shown to mimic the protective effects of ischemic preconditioning and mitigate ischemia-reperfusion (I/R) injury in the heart, brain, gast...
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Elsevier
2016-12-01
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| Series: | Pediatrics and Neonatology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1875957216000310 |
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| author | Chih-Cheng Luo Chen-Sheng Huang Yung-Ching Ming Shih-Ming Chu Hsun-Chin Chao |
| author_facet | Chih-Cheng Luo Chen-Sheng Huang Yung-Ching Ming Shih-Ming Chu Hsun-Chin Chao |
| author_sort | Chih-Cheng Luo |
| collection | DOAJ |
| description | Various investigations have demonstrated that calcitonin gene-related peptide (CGRP) plays an important role in mediating ischemic preconditioning. CGRP has been shown to mimic the protective effects of ischemic preconditioning and mitigate ischemia-reperfusion (I/R) injury in the heart, brain, gastrointestinal system, and other tissues. This study aimed to examine whether CGRP, a proven intestinal cytoprotective molecule, exerted its protective effects through modulation of inducible nitride oxide synthase (iNOS) and apoptosis after intestinal I/R injury.
Methods: This animal study randomly divided 30 rats into the following five groups: (1) the normal control group, (2) the ischemia group with normal saline, (3) the I/R group with normal saline, (4) the ischemia group with CGRP (300 μg/kg), and (5) the I/R group with CGRP (300 μg/kg). Levels of iNOS messenger RNA (mRNA) and protein, and caspase-3 protein were determined by real-time quantitative polymerase chain reaction and Western blotting analyses, respectively. Statistical analysis was performed using analysis of variance with Dunn test.
Results: The mRNA levels of iNOS increased after the intestinal ischemia or intestinal reperfusion phase (p < 0.01), and CGRP pretreatment significantly decreased iNOS mRNAs and protein levels (p < 0.01). The expression protein levels of caspase-3 increased after the intestinal ischemia or intestinal reperfusion phase. CGRP pretreatment significantly decreased the levels of caspase-3 proteins. CGRP intestinal cytoprotection is mediated, in part, by downregulation of expression of iNOS and caspase-3 after intestinal I/R injury.
Conclusion: The study indicates that the cytoprotective role of CGRP (i.e., antiapoptotic effect) after I/R injury could be via downregulation of iNOS, which may relieve I/R tissue damage by blocking iNOS activity. |
| format | Article |
| id | doaj-art-9e7502bfbc674234aff14f84cfbf499a |
| institution | OA Journals |
| issn | 1875-9572 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pediatrics and Neonatology |
| spelling | doaj-art-9e7502bfbc674234aff14f84cfbf499a2025-08-20T02:18:32ZengElsevierPediatrics and Neonatology1875-95722016-12-0157647447910.1016/j.pedneo.2015.10.012Calcitonin Gene-Related Peptide Downregulates Expression of Inducible Nitride Oxide Synthase and Caspase-3 after Intestinal Ischemia-Reperfusion Injury in RatsChih-Cheng Luo0Chen-Sheng Huang1Yung-Ching Ming2Shih-Ming Chu3Hsun-Chin Chao4Division of Pediatric Surgery, Department of Surgery, Taipei Municipal Wan Fang Hospital, Taipei Medical University, Taipei City, TaiwanDivision of Pediatric Surgery, Department of Surgery, Taipei Municipal Wan Fang Hospital, Taipei Medical University, Taipei City, TaiwanDepartment of Pediatric Surgery, Chang Gung Children's Medical Center, Chang Gung Memorial Hospital, Guishan, Taoyuan, TaiwanDivision of Neonatology, Chang Gung Children's Medical Center, Chang Gung Memorial Hospital, Guishan, Taoyuan, TaiwanDivision of Pediatric Gastroenterology, Chang Gung Children's Medical Center, Chang Gung Memorial Hospital, Guishan, Taoyuan, TaiwanVarious investigations have demonstrated that calcitonin gene-related peptide (CGRP) plays an important role in mediating ischemic preconditioning. CGRP has been shown to mimic the protective effects of ischemic preconditioning and mitigate ischemia-reperfusion (I/R) injury in the heart, brain, gastrointestinal system, and other tissues. This study aimed to examine whether CGRP, a proven intestinal cytoprotective molecule, exerted its protective effects through modulation of inducible nitride oxide synthase (iNOS) and apoptosis after intestinal I/R injury. Methods: This animal study randomly divided 30 rats into the following five groups: (1) the normal control group, (2) the ischemia group with normal saline, (3) the I/R group with normal saline, (4) the ischemia group with CGRP (300 μg/kg), and (5) the I/R group with CGRP (300 μg/kg). Levels of iNOS messenger RNA (mRNA) and protein, and caspase-3 protein were determined by real-time quantitative polymerase chain reaction and Western blotting analyses, respectively. Statistical analysis was performed using analysis of variance with Dunn test. Results: The mRNA levels of iNOS increased after the intestinal ischemia or intestinal reperfusion phase (p < 0.01), and CGRP pretreatment significantly decreased iNOS mRNAs and protein levels (p < 0.01). The expression protein levels of caspase-3 increased after the intestinal ischemia or intestinal reperfusion phase. CGRP pretreatment significantly decreased the levels of caspase-3 proteins. CGRP intestinal cytoprotection is mediated, in part, by downregulation of expression of iNOS and caspase-3 after intestinal I/R injury. Conclusion: The study indicates that the cytoprotective role of CGRP (i.e., antiapoptotic effect) after I/R injury could be via downregulation of iNOS, which may relieve I/R tissue damage by blocking iNOS activity.http://www.sciencedirect.com/science/article/pii/S1875957216000310calcitonin gene-related peptidecaspase-3inducible nitride oxide synthaseischemia-reperfusion injurysmall intestine |
| spellingShingle | Chih-Cheng Luo Chen-Sheng Huang Yung-Ching Ming Shih-Ming Chu Hsun-Chin Chao Calcitonin Gene-Related Peptide Downregulates Expression of Inducible Nitride Oxide Synthase and Caspase-3 after Intestinal Ischemia-Reperfusion Injury in Rats Pediatrics and Neonatology calcitonin gene-related peptide caspase-3 inducible nitride oxide synthase ischemia-reperfusion injury small intestine |
| title | Calcitonin Gene-Related Peptide Downregulates Expression of Inducible Nitride Oxide Synthase and Caspase-3 after Intestinal Ischemia-Reperfusion Injury in Rats |
| title_full | Calcitonin Gene-Related Peptide Downregulates Expression of Inducible Nitride Oxide Synthase and Caspase-3 after Intestinal Ischemia-Reperfusion Injury in Rats |
| title_fullStr | Calcitonin Gene-Related Peptide Downregulates Expression of Inducible Nitride Oxide Synthase and Caspase-3 after Intestinal Ischemia-Reperfusion Injury in Rats |
| title_full_unstemmed | Calcitonin Gene-Related Peptide Downregulates Expression of Inducible Nitride Oxide Synthase and Caspase-3 after Intestinal Ischemia-Reperfusion Injury in Rats |
| title_short | Calcitonin Gene-Related Peptide Downregulates Expression of Inducible Nitride Oxide Synthase and Caspase-3 after Intestinal Ischemia-Reperfusion Injury in Rats |
| title_sort | calcitonin gene related peptide downregulates expression of inducible nitride oxide synthase and caspase 3 after intestinal ischemia reperfusion injury in rats |
| topic | calcitonin gene-related peptide caspase-3 inducible nitride oxide synthase ischemia-reperfusion injury small intestine |
| url | http://www.sciencedirect.com/science/article/pii/S1875957216000310 |
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