<i>Artemisia annua</i> Residue Regulates Immunity, Antioxidant Ability, Intestinal Barrier Function, and Microbial Structure in Weaned Piglets

<i>Artemisia annua</i> residue (AR), as the byproduct of industrial extraction of artemisinin, contains rich nutrients and active ingredients. This study was conducted to determine the effects of AR as an unconventional feed material on growth performance, immunity, and intestinal health...

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Main Authors: Jinjie Hu, Miaomiao Bai, Yueyao Xing, Junhong Liu, Kang Xu, Xia Xiong, Hongnan Liu, Yulong Yin
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/14/24/3569
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author Jinjie Hu
Miaomiao Bai
Yueyao Xing
Junhong Liu
Kang Xu
Xia Xiong
Hongnan Liu
Yulong Yin
author_facet Jinjie Hu
Miaomiao Bai
Yueyao Xing
Junhong Liu
Kang Xu
Xia Xiong
Hongnan Liu
Yulong Yin
author_sort Jinjie Hu
collection DOAJ
description <i>Artemisia annua</i> residue (AR), as the byproduct of industrial extraction of artemisinin, contains rich nutrients and active ingredients. This study was conducted to determine the effects of AR as an unconventional feed material on growth performance, immunity, and intestinal health in weaned piglets. Thirty-two piglets weaned at 21 days (7.53 ± 0.31 kg average BW) were fed with a corn–soybean basal diet (BD) and a basal diet with 1% (LAR), 2% (MAR), and 4% (HAR) AR diets for 28 days. AR diets increased the serum IgA and complement component 3 levels, superoxide dismutase activity, and villus height in the duodenum (<i>p</i> < 0.05). The MAR group increased the ADG, serum total protein, and mRNA expression levels of Claudin-1 in the duodenum and zonula occludens-1 (ZO-1) and the mucin 2 (MUC2) in the colon, as well as colonic <i>Romboutsia</i> and <i>Anaerostipes</i> abundances, and decreased the <i>Proteobacteria</i> abundance (<i>p</i> < 0.05). To sum up, dietary AR supplementation may enhance growth performance by improving serum immunoglobulin and antioxidant enzyme activity, intestinal morphology, tight junction protein expression, and gut microbiota of weaned piglets. Regression analysis showed that the optimal AR supplemental level for growth performance, immunity, antioxidant ability, and intestinal health of weaned piglets was 2.08% to 4.24%.
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spelling doaj-art-9e718a2d03614d63aeb312b5c632e6772025-08-20T02:55:49ZengMDPI AGAnimals2076-26152024-12-011424356910.3390/ani14243569<i>Artemisia annua</i> Residue Regulates Immunity, Antioxidant Ability, Intestinal Barrier Function, and Microbial Structure in Weaned PigletsJinjie Hu0Miaomiao Bai1Yueyao Xing2Junhong Liu3Kang Xu4Xia Xiong5Hongnan Liu6Yulong Yin7College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaLaboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaLaboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaLaboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaLaboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China<i>Artemisia annua</i> residue (AR), as the byproduct of industrial extraction of artemisinin, contains rich nutrients and active ingredients. This study was conducted to determine the effects of AR as an unconventional feed material on growth performance, immunity, and intestinal health in weaned piglets. Thirty-two piglets weaned at 21 days (7.53 ± 0.31 kg average BW) were fed with a corn–soybean basal diet (BD) and a basal diet with 1% (LAR), 2% (MAR), and 4% (HAR) AR diets for 28 days. AR diets increased the serum IgA and complement component 3 levels, superoxide dismutase activity, and villus height in the duodenum (<i>p</i> < 0.05). The MAR group increased the ADG, serum total protein, and mRNA expression levels of Claudin-1 in the duodenum and zonula occludens-1 (ZO-1) and the mucin 2 (MUC2) in the colon, as well as colonic <i>Romboutsia</i> and <i>Anaerostipes</i> abundances, and decreased the <i>Proteobacteria</i> abundance (<i>p</i> < 0.05). To sum up, dietary AR supplementation may enhance growth performance by improving serum immunoglobulin and antioxidant enzyme activity, intestinal morphology, tight junction protein expression, and gut microbiota of weaned piglets. Regression analysis showed that the optimal AR supplemental level for growth performance, immunity, antioxidant ability, and intestinal health of weaned piglets was 2.08% to 4.24%.https://www.mdpi.com/2076-2615/14/24/3569<i>Artemisia annua</i> residueimmunityintestinal barrier functionintestinal microbiotaweaned piglets
spellingShingle Jinjie Hu
Miaomiao Bai
Yueyao Xing
Junhong Liu
Kang Xu
Xia Xiong
Hongnan Liu
Yulong Yin
<i>Artemisia annua</i> Residue Regulates Immunity, Antioxidant Ability, Intestinal Barrier Function, and Microbial Structure in Weaned Piglets
Animals
<i>Artemisia annua</i> residue
immunity
intestinal barrier function
intestinal microbiota
weaned piglets
title <i>Artemisia annua</i> Residue Regulates Immunity, Antioxidant Ability, Intestinal Barrier Function, and Microbial Structure in Weaned Piglets
title_full <i>Artemisia annua</i> Residue Regulates Immunity, Antioxidant Ability, Intestinal Barrier Function, and Microbial Structure in Weaned Piglets
title_fullStr <i>Artemisia annua</i> Residue Regulates Immunity, Antioxidant Ability, Intestinal Barrier Function, and Microbial Structure in Weaned Piglets
title_full_unstemmed <i>Artemisia annua</i> Residue Regulates Immunity, Antioxidant Ability, Intestinal Barrier Function, and Microbial Structure in Weaned Piglets
title_short <i>Artemisia annua</i> Residue Regulates Immunity, Antioxidant Ability, Intestinal Barrier Function, and Microbial Structure in Weaned Piglets
title_sort i artemisia annua i residue regulates immunity antioxidant ability intestinal barrier function and microbial structure in weaned piglets
topic <i>Artemisia annua</i> residue
immunity
intestinal barrier function
intestinal microbiota
weaned piglets
url https://www.mdpi.com/2076-2615/14/24/3569
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