Annexin A8 deficiency delays atherosclerosis progression
Abstract Background Atherosclerosis is a chronic inflammatory disease characterized by the accumulation of lipids and leukocytes within the arterial wall. By studying the aortic transcriptome of atherosclerosis‐prone apolipoprotein E (ApoE−/−) mice, we aimed to identify novel players in the progress...
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Wiley
2025-01-01
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| Series: | Clinical and Translational Medicine |
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| Online Access: | https://doi.org/10.1002/ctm2.70176 |
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| author | Carmen Gutiérrez‐Muñoz Rafael Blázquez‐Serra Irene San Sebastian‐Jaraba Sandra Sanz‐Andrea Maria J. Fernández‐Gómez Gonzalo Nuñez‐Moreno Pablo Mínguez Joan Carles Escolá‐Gil Paula Nogales Veronique Ollivier Jose L. Martín‐Ventura Benoit Ho‐Tin Noe Ursula Rescher Nerea Méndez‐Barbero Luis M. Blanco‐Colio |
| author_facet | Carmen Gutiérrez‐Muñoz Rafael Blázquez‐Serra Irene San Sebastian‐Jaraba Sandra Sanz‐Andrea Maria J. Fernández‐Gómez Gonzalo Nuñez‐Moreno Pablo Mínguez Joan Carles Escolá‐Gil Paula Nogales Veronique Ollivier Jose L. Martín‐Ventura Benoit Ho‐Tin Noe Ursula Rescher Nerea Méndez‐Barbero Luis M. Blanco‐Colio |
| author_sort | Carmen Gutiérrez‐Muñoz |
| collection | DOAJ |
| description | Abstract Background Atherosclerosis is a chronic inflammatory disease characterized by the accumulation of lipids and leukocytes within the arterial wall. By studying the aortic transcriptome of atherosclerosis‐prone apolipoprotein E (ApoE−/−) mice, we aimed to identify novel players in the progression of atherosclerosis. Methods RNA‐Seq analysis was performed on aortas from ApoE−/− and wild‐type mice. AnxA8 expression was assessed in human and mice atherosclerotic tissue and healthy aorta. ApoE−/− mice lacking systemic AnxA8 (ApoE−/−AnxA8−/−) were generated to assess the effect of AnxA8 deficiency on atherosclerosis. Bone marrow transplantation (BMT) was also performed to generate ApoE−/− lacking AnxA8 specifically in bone marrow‐derived cells. Endothelial‐specific AnxA8 silencing in vivo was performed in ApoE−/− mice. The functional role of AnxA8 was analysed in cultured murine cells. Results RNA‐Seq unveiled AnxA8 as one of the most significantly upregulated genes in atherosclerotic aortas of ApoE−/− compared to wild‐type mice. Moreover, AnxA8 was upregulated in human atherosclerotic plaques. Germline deletion of AnxA8 decreased the atherosclerotic burden, the size and volume of atherosclerotic plaques in the aortic root. Plaques of ApoE−/−AnxA8−/− were characterized by lower lipid and inflammatory content, smaller necrotic core, thicker fibrous cap and less apoptosis compared with those in ApoE−/−AnxA8+/+. BMT showed that hematopoietic AnxA8 deficiency had no effect on atherosclerotic progression. Oxidized low‐density lipoprotein (ox‐LDL) increased AnxA8 expression in murine aortic endothelial cells (MAECs). In vitro experiments revealed that AnxA8 deficiency in MAECs suppressed P/E‐selectin and CD31 expression and secretion induced by ox‐LDL with a concomitant reduction in platelet and leukocyte adhesion. Intravital microscopy confirmed the reduction in leukocyte and platelet adhesion in ApoE−/−AnxA8−/− mice. Finally, endothelial‐specific silencing of AnxA8 decreased atherosclerosis progression. Conclusion Our findings demonstrate that AnxA8 promotes the progression of atherosclerosis by modulating endothelial−leukocyte interactions. Interventions capable of reducing AnxA8 expression in endothelial cells may delay atherosclerotic plaque progression. Key points This study shows that AnxA8 is upregulated in aorta of atheroprone mice and in human atherosclerotic plaques. Germline AnxA8 deficiency reduces platelet and leukocyte recruitment to activated endothelium as well as atherosclerotic burden, plaque size, and macrophage accumulation in mice. AnxA8 regulates oxLDL‐induced adhesion molecules expression in aortic endothelial cells. Our data strongly suggest that AnxA8 promotes disease progression through regulation of adhesion and influx of immune cells to the intima. Endothelial specific silencing of AnxA8 reduced atherosclerosis progression. Therapeutic interventions to reduce AnxA8 expression may delay atherosclerosis progression. |
| format | Article |
| id | doaj-art-9e63958f007e4a0797c1405762a31340 |
| institution | Kabale University |
| issn | 2001-1326 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Medicine |
| spelling | doaj-art-9e63958f007e4a0797c1405762a313402025-01-25T04:00:39ZengWileyClinical and Translational Medicine2001-13262025-01-01151n/an/a10.1002/ctm2.70176Annexin A8 deficiency delays atherosclerosis progressionCarmen Gutiérrez‐Muñoz0Rafael Blázquez‐Serra1Irene San Sebastian‐Jaraba2Sandra Sanz‐Andrea3Maria J. Fernández‐Gómez4Gonzalo Nuñez‐Moreno5Pablo Mínguez6Joan Carles Escolá‐Gil7Paula Nogales8Veronique Ollivier9Jose L. Martín‐Ventura10Benoit Ho‐Tin Noe11Ursula Rescher12Nerea Méndez‐Barbero13Luis M. Blanco‐Colio14Vascular Research Laboratory, IIS‐Fundación Jiménez Díaz Madrid SpainVascular Research Laboratory, IIS‐Fundación Jiménez Díaz Madrid SpainVascular Research Laboratory, IIS‐Fundación Jiménez Díaz Madrid SpainVascular Research Laboratory, IIS‐Fundación Jiménez Díaz Madrid SpainVascular Research Laboratory, IIS‐Fundación Jiménez Díaz Madrid SpainBioinformatics Unit Department of Genetics & Genomics IIS‐Fundación Jiménez Díaz Madrid SpainBioinformatics Unit Department of Genetics & Genomics IIS‐Fundación Jiménez Díaz Madrid SpainInstitut d'Investigacions Biomèdiques (IIB) Sant Pau Barcelona SpainCentro Nacional de Investigaciones Cardiovasculares Madrid SpainLaboratory for Vascular Translation Science Inserm U1148, Paris Bichat Hospital Paris FranceVascular Research Laboratory, IIS‐Fundación Jiménez Díaz Madrid SpainLaboratory for Vascular Translation Science Inserm U1148, Paris Bichat Hospital Paris FranceCenter for Molecular Biology of Inflammation Research Group Regulatory Mechanisms of Inflammation, Institute of Medical Biochemistry, University of Muenster Muenster GermanyVascular Research Laboratory, IIS‐Fundación Jiménez Díaz Madrid SpainVascular Research Laboratory, IIS‐Fundación Jiménez Díaz Madrid SpainAbstract Background Atherosclerosis is a chronic inflammatory disease characterized by the accumulation of lipids and leukocytes within the arterial wall. By studying the aortic transcriptome of atherosclerosis‐prone apolipoprotein E (ApoE−/−) mice, we aimed to identify novel players in the progression of atherosclerosis. Methods RNA‐Seq analysis was performed on aortas from ApoE−/− and wild‐type mice. AnxA8 expression was assessed in human and mice atherosclerotic tissue and healthy aorta. ApoE−/− mice lacking systemic AnxA8 (ApoE−/−AnxA8−/−) were generated to assess the effect of AnxA8 deficiency on atherosclerosis. Bone marrow transplantation (BMT) was also performed to generate ApoE−/− lacking AnxA8 specifically in bone marrow‐derived cells. Endothelial‐specific AnxA8 silencing in vivo was performed in ApoE−/− mice. The functional role of AnxA8 was analysed in cultured murine cells. Results RNA‐Seq unveiled AnxA8 as one of the most significantly upregulated genes in atherosclerotic aortas of ApoE−/− compared to wild‐type mice. Moreover, AnxA8 was upregulated in human atherosclerotic plaques. Germline deletion of AnxA8 decreased the atherosclerotic burden, the size and volume of atherosclerotic plaques in the aortic root. Plaques of ApoE−/−AnxA8−/− were characterized by lower lipid and inflammatory content, smaller necrotic core, thicker fibrous cap and less apoptosis compared with those in ApoE−/−AnxA8+/+. BMT showed that hematopoietic AnxA8 deficiency had no effect on atherosclerotic progression. Oxidized low‐density lipoprotein (ox‐LDL) increased AnxA8 expression in murine aortic endothelial cells (MAECs). In vitro experiments revealed that AnxA8 deficiency in MAECs suppressed P/E‐selectin and CD31 expression and secretion induced by ox‐LDL with a concomitant reduction in platelet and leukocyte adhesion. Intravital microscopy confirmed the reduction in leukocyte and platelet adhesion in ApoE−/−AnxA8−/− mice. Finally, endothelial‐specific silencing of AnxA8 decreased atherosclerosis progression. Conclusion Our findings demonstrate that AnxA8 promotes the progression of atherosclerosis by modulating endothelial−leukocyte interactions. Interventions capable of reducing AnxA8 expression in endothelial cells may delay atherosclerotic plaque progression. Key points This study shows that AnxA8 is upregulated in aorta of atheroprone mice and in human atherosclerotic plaques. Germline AnxA8 deficiency reduces platelet and leukocyte recruitment to activated endothelium as well as atherosclerotic burden, plaque size, and macrophage accumulation in mice. AnxA8 regulates oxLDL‐induced adhesion molecules expression in aortic endothelial cells. Our data strongly suggest that AnxA8 promotes disease progression through regulation of adhesion and influx of immune cells to the intima. Endothelial specific silencing of AnxA8 reduced atherosclerosis progression. Therapeutic interventions to reduce AnxA8 expression may delay atherosclerosis progression.https://doi.org/10.1002/ctm2.70176AnxA8atherosclerosisinflammation |
| spellingShingle | Carmen Gutiérrez‐Muñoz Rafael Blázquez‐Serra Irene San Sebastian‐Jaraba Sandra Sanz‐Andrea Maria J. Fernández‐Gómez Gonzalo Nuñez‐Moreno Pablo Mínguez Joan Carles Escolá‐Gil Paula Nogales Veronique Ollivier Jose L. Martín‐Ventura Benoit Ho‐Tin Noe Ursula Rescher Nerea Méndez‐Barbero Luis M. Blanco‐Colio Annexin A8 deficiency delays atherosclerosis progression Clinical and Translational Medicine AnxA8 atherosclerosis inflammation |
| title | Annexin A8 deficiency delays atherosclerosis progression |
| title_full | Annexin A8 deficiency delays atherosclerosis progression |
| title_fullStr | Annexin A8 deficiency delays atherosclerosis progression |
| title_full_unstemmed | Annexin A8 deficiency delays atherosclerosis progression |
| title_short | Annexin A8 deficiency delays atherosclerosis progression |
| title_sort | annexin a8 deficiency delays atherosclerosis progression |
| topic | AnxA8 atherosclerosis inflammation |
| url | https://doi.org/10.1002/ctm2.70176 |
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