Diversity analysis on gut microbiota of Glyphodes pyloalis Walker under high temperature
To explore the relationship between stress-resistance physiology and gut microbiota of Glyphodes pyloalis Walker, high-throughput sequencing technique was performed to measure the sequence of 16S rDNA-V4 variable region of gut bacteria in G. pyloalis before and after high temperature. The difference...
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| Language: | English |
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Zhejiang University Press
2018-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.04.082 |
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| author | LIANG Yanting LIU Yuncai GAO Yun WANG Huabing XU Yusong |
| author_facet | LIANG Yanting LIU Yuncai GAO Yun WANG Huabing XU Yusong |
| author_sort | LIANG Yanting |
| collection | DOAJ |
| description | To explore the relationship between stress-resistance physiology and gut microbiota of Glyphodes pyloalis Walker, high-throughput sequencing technique was performed to measure the sequence of 16S rDNA-V4 variable region of gut bacteria in G. pyloalis before and after high temperature. The differences of bacterial communities in composition and bacterial diversity were analyzed, and the effects of gut microbiota changes on its stress-resistance physiology were clarified under high temperature. The results showed that at the level of phylum, Acidobacteria was not detected after the high temperature treatment, but Fusobacteria, Planctomycetes and Tenericutes were newly observed. At the level of genus, the abundance of Wolbachia increased by 26.38%, with the greatest change. Among the dominant bacterial communities in the gut of G. pyloalis, Pseudomonas had the most significant change after the high temperature treatment, then followed by Prevotella, while the abundances of Arthrobacter, Cellulophaga, Escherichia and Parabacteroides were decreased, indicating that high temperature inhibited the reproduction of bacillus but promoted the reproduction of other bacteria. This study shows that after the high temperature treatment, the diversity and structure of gut bacterial communities in G. pyloalis both have significant changes, which increase the stress-resistance of G. pyloalis to better adapt to high temperature environment. |
| format | Article |
| id | doaj-art-9e4e6b08a919438e91598bd3947f5929 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-9e4e6b08a919438e91598bd3947f59292025-08-20T02:47:32ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552018-03-014421522210.3785/j.issn.1008-9209.2018.04.08210089209Diversity analysis on gut microbiota of Glyphodes pyloalis Walker under high temperatureLIANG YantingLIU YuncaiGAO YunWANG HuabingXU YusongTo explore the relationship between stress-resistance physiology and gut microbiota of Glyphodes pyloalis Walker, high-throughput sequencing technique was performed to measure the sequence of 16S rDNA-V4 variable region of gut bacteria in G. pyloalis before and after high temperature. The differences of bacterial communities in composition and bacterial diversity were analyzed, and the effects of gut microbiota changes on its stress-resistance physiology were clarified under high temperature. The results showed that at the level of phylum, Acidobacteria was not detected after the high temperature treatment, but Fusobacteria, Planctomycetes and Tenericutes were newly observed. At the level of genus, the abundance of Wolbachia increased by 26.38%, with the greatest change. Among the dominant bacterial communities in the gut of G. pyloalis, Pseudomonas had the most significant change after the high temperature treatment, then followed by Prevotella, while the abundances of Arthrobacter, Cellulophaga, Escherichia and Parabacteroides were decreased, indicating that high temperature inhibited the reproduction of bacillus but promoted the reproduction of other bacteria. This study shows that after the high temperature treatment, the diversity and structure of gut bacterial communities in G. pyloalis both have significant changes, which increase the stress-resistance of G. pyloalis to better adapt to high temperature environment.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.04.082<italic>Glyphodes pyloalis</italic> Walkergut microbiotahigh-throughput sequencinghigh temperaturediversity |
| spellingShingle | LIANG Yanting LIU Yuncai GAO Yun WANG Huabing XU Yusong Diversity analysis on gut microbiota of Glyphodes pyloalis Walker under high temperature 浙江大学学报. 农业与生命科学版 <italic>Glyphodes pyloalis</italic> Walker gut microbiota high-throughput sequencing high temperature diversity |
| title | Diversity analysis on gut microbiota of Glyphodes pyloalis Walker under high temperature |
| title_full | Diversity analysis on gut microbiota of Glyphodes pyloalis Walker under high temperature |
| title_fullStr | Diversity analysis on gut microbiota of Glyphodes pyloalis Walker under high temperature |
| title_full_unstemmed | Diversity analysis on gut microbiota of Glyphodes pyloalis Walker under high temperature |
| title_short | Diversity analysis on gut microbiota of Glyphodes pyloalis Walker under high temperature |
| title_sort | diversity analysis on gut microbiota of glyphodes pyloalis walker under high temperature |
| topic | <italic>Glyphodes pyloalis</italic> Walker gut microbiota high-throughput sequencing high temperature diversity |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.04.082 |
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