Ultrasensitive Detection of Neurotransmitter in Neurological Disorders
Psychological or neurological disorders have worldwide prevalence of 10-13 %. In Pakistan the situation is equally alarming affecting 10-16% of the population. Among all the neurological diseases the most prevalent one in Pakistani population are: depression (6%), schizophrenia (1.5%) and epilepsy...
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| Format: | Article |
| Language: | English |
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National University of Sciences and Technology, Islamabad
2024-12-01
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| Series: | NUST Journal of Engineering Sciences |
| Online Access: | https://journals.nust.edu.pk/index.php/njes/article/view/845 |
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| author | Kiran Kayani Hijab Fatima Fahad Bhopal Jamshed Aftab Naureen Ehsan Ilahi Asma Gul Dr Madeeha Chaudhry |
| author_facet | Kiran Kayani Hijab Fatima Fahad Bhopal Jamshed Aftab Naureen Ehsan Ilahi Asma Gul Dr Madeeha Chaudhry |
| author_sort | Kiran Kayani |
| collection | DOAJ |
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Psychological or neurological disorders have worldwide prevalence of 10-13 %. In Pakistan the situation is equally alarming affecting 10-16% of the population. Among all the neurological diseases the most prevalent one in Pakistani population are: depression (6%), schizophrenia (1.5%) and epilepsy (1-2%). Dopamine, a critical neurotransmitter plays an important role in onset and progression of aforementioned disorders. Normal dopamine levels in human blood are 0 to 30 pg/mL (195.8 pmol/L). A little variation in its levels can cause onset of depression and various neurological diseases. Conventional dopamine detection techniques are laborious and time consuming that’s why less commonly practiced in Pakistani clinics. We propose an immunoassay chip offering optical detection of different log dilutions (1/10, 1/100 and 1/1000) of dopamine using CdSe/ZnS quantum dots (QDs). Elucidation of fluorescence response of different dilutions of quantum dot conjugated dopamine antibody revealed maximum fluorescence intensity at 1/10 dilution. This demonstrates the efficiency and sensitivity of the current method. The current study would be revolutionary in proposing a timely diagnosis of dopamine levels to patients suffering from neurological problems. Similarly, facilitating timely diagnosis and personalized treatment for patients with neurological disorders.
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| format | Article |
| id | doaj-art-9e4bde007803449dbea9cb00c96c5e90 |
| institution | DOAJ |
| issn | 2070-9900 2411-6319 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | National University of Sciences and Technology, Islamabad |
| record_format | Article |
| series | NUST Journal of Engineering Sciences |
| spelling | doaj-art-9e4bde007803449dbea9cb00c96c5e902025-08-20T03:18:15ZengNational University of Sciences and Technology, IslamabadNUST Journal of Engineering Sciences2070-99002411-63192024-12-0117210.24949/njes.v17i2.845Ultrasensitive Detection of Neurotransmitter in Neurological DisordersKiran Kayani0Hijab Fatima1Fahad Bhopal2Jamshed Aftab3Naureen Ehsan Ilahi4Asma Gul5Dr Madeeha Chaudhry6Department of Physics, COMSATS University, IslamabadDepartment of Physics, COMSATS University, IslamabadDepartment of Physics, COMSATS University, IslamabadDepartment of Physics, Virtual University of Pakistan, IslamabadDepartment of Biological Sciences, Virtual University of Pakistan, IslamabadDepartment of Biological Sciences, International Islamic University, IslamabadDepartment of Biological Sciences, Virtual University of Pakistan, Islamabad Psychological or neurological disorders have worldwide prevalence of 10-13 %. In Pakistan the situation is equally alarming affecting 10-16% of the population. Among all the neurological diseases the most prevalent one in Pakistani population are: depression (6%), schizophrenia (1.5%) and epilepsy (1-2%). Dopamine, a critical neurotransmitter plays an important role in onset and progression of aforementioned disorders. Normal dopamine levels in human blood are 0 to 30 pg/mL (195.8 pmol/L). A little variation in its levels can cause onset of depression and various neurological diseases. Conventional dopamine detection techniques are laborious and time consuming that’s why less commonly practiced in Pakistani clinics. We propose an immunoassay chip offering optical detection of different log dilutions (1/10, 1/100 and 1/1000) of dopamine using CdSe/ZnS quantum dots (QDs). Elucidation of fluorescence response of different dilutions of quantum dot conjugated dopamine antibody revealed maximum fluorescence intensity at 1/10 dilution. This demonstrates the efficiency and sensitivity of the current method. The current study would be revolutionary in proposing a timely diagnosis of dopamine levels to patients suffering from neurological problems. Similarly, facilitating timely diagnosis and personalized treatment for patients with neurological disorders. https://journals.nust.edu.pk/index.php/njes/article/view/845 |
| spellingShingle | Kiran Kayani Hijab Fatima Fahad Bhopal Jamshed Aftab Naureen Ehsan Ilahi Asma Gul Dr Madeeha Chaudhry Ultrasensitive Detection of Neurotransmitter in Neurological Disorders NUST Journal of Engineering Sciences |
| title | Ultrasensitive Detection of Neurotransmitter in Neurological Disorders |
| title_full | Ultrasensitive Detection of Neurotransmitter in Neurological Disorders |
| title_fullStr | Ultrasensitive Detection of Neurotransmitter in Neurological Disorders |
| title_full_unstemmed | Ultrasensitive Detection of Neurotransmitter in Neurological Disorders |
| title_short | Ultrasensitive Detection of Neurotransmitter in Neurological Disorders |
| title_sort | ultrasensitive detection of neurotransmitter in neurological disorders |
| url | https://journals.nust.edu.pk/index.php/njes/article/view/845 |
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