Oncolytic potential of Newcastle Disease Virus in feline lymphoma cells: an in vitro evaluation
Lymphoma is one of the most prevalent types of feline cancer. It is characterized as a group of diseases that can affect various organs, such as the gastrointestinal tract, kidneys, thymus, and skin. In feline medicine, the search for alternative treatments is of utmost importance, given the signifi...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-06-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1484947/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850119349604450304 |
|---|---|
| author | Talita Gabriela Luna Alves Pedro Luiz Porfirio Xavier Taismara Kustro Garnica Arina Lázaro Rochetti Talal Jamil Qazi Thiago Henrique Annibale Vendramini Felipe Haddad Muhammad Munir Márcio Antonio Brunetto Heidge Fukumasu |
| author_facet | Talita Gabriela Luna Alves Pedro Luiz Porfirio Xavier Taismara Kustro Garnica Arina Lázaro Rochetti Talal Jamil Qazi Thiago Henrique Annibale Vendramini Felipe Haddad Muhammad Munir Márcio Antonio Brunetto Heidge Fukumasu |
| author_sort | Talita Gabriela Luna Alves |
| collection | DOAJ |
| description | Lymphoma is one of the most prevalent types of feline cancer. It is characterized as a group of diseases that can affect various organs, such as the gastrointestinal tract, kidneys, thymus, and skin. In feline medicine, the search for alternative treatments is of utmost importance, given the significant number of animals that relapse or are unresponsive to conventional chemotherapy treatment. As an alternative to existing modalities of treatment for a variety of cancers, oncolytic viruses have been studied in the last few years. Those viruses possess a unique ability to target and eliminate cancer cells while simultaneously stimulating an immune response against malignant cells, acting as an immunotherapy. Newcastle Disease Virus (NDV) is an avian paramyxovirus that affects both domestic and wild birds, causing symptoms that range from severe to asymptomatic, depending on the viral strain. Less virulent strains are considered safe for use as a vaccine against Newcastle Disease. In the Oncology field, those strains are also being studied to be used as oncolytic virotherapy for mammals, and several results demonstrate their efficacy in vitro and in vivo. The present study aimed to explore the oncolytic potential of Newcastle Disease Virus expressing green fluorescent protein (NDV-GFP) in feline lymphoma cells isolated from a FeLV-positive patient with thymic lymphoma. The NDV-GFP infected, replicated, and induced apoptosis in feline lymphoma cells. Therefore, these results provide preliminary evidence of the oncolytic activity of NDV in feline leukemia virus-induced lymphoma. |
| format | Article |
| id | doaj-art-9e4315fb7f334549b77d3a8db812f0f1 |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-9e4315fb7f334549b77d3a8db812f0f12025-08-20T02:35:39ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-06-011210.3389/fvets.2025.14849471484947Oncolytic potential of Newcastle Disease Virus in feline lymphoma cells: an in vitro evaluationTalita Gabriela Luna Alves0Pedro Luiz Porfirio Xavier1Taismara Kustro Garnica2Arina Lázaro Rochetti3Talal Jamil Qazi4Thiago Henrique Annibale Vendramini5Felipe Haddad6Muhammad Munir7Márcio Antonio Brunetto8Heidge Fukumasu9Laboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo (USP), Pirassununga, BrazilLaboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo (USP), Pirassununga, BrazilLaboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo (USP), Pirassununga, BrazilLaboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo (USP), Pirassununga, BrazilLaboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo (USP), Pirassununga, BrazilNutrition and Production Department, School of Veterinary Medicine and Animal Science, Pet Nutrology Research Center, University of São Paulo (USP), Pirassununga, BrazilAdvanced Cellular Therapy Laboratory, Ribeirao Preto Blood Center Foundation, University of São Paulo (USP), Ribeirao Preto, BrazilDivision of Biomedical and Life Sciences, Lancaster University, Lancaster, United KingdomNutrition and Production Department, School of Veterinary Medicine and Animal Science, Pet Nutrology Research Center, University of São Paulo (USP), Pirassununga, BrazilLaboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo (USP), Pirassununga, BrazilLymphoma is one of the most prevalent types of feline cancer. It is characterized as a group of diseases that can affect various organs, such as the gastrointestinal tract, kidneys, thymus, and skin. In feline medicine, the search for alternative treatments is of utmost importance, given the significant number of animals that relapse or are unresponsive to conventional chemotherapy treatment. As an alternative to existing modalities of treatment for a variety of cancers, oncolytic viruses have been studied in the last few years. Those viruses possess a unique ability to target and eliminate cancer cells while simultaneously stimulating an immune response against malignant cells, acting as an immunotherapy. Newcastle Disease Virus (NDV) is an avian paramyxovirus that affects both domestic and wild birds, causing symptoms that range from severe to asymptomatic, depending on the viral strain. Less virulent strains are considered safe for use as a vaccine against Newcastle Disease. In the Oncology field, those strains are also being studied to be used as oncolytic virotherapy for mammals, and several results demonstrate their efficacy in vitro and in vivo. The present study aimed to explore the oncolytic potential of Newcastle Disease Virus expressing green fluorescent protein (NDV-GFP) in feline lymphoma cells isolated from a FeLV-positive patient with thymic lymphoma. The NDV-GFP infected, replicated, and induced apoptosis in feline lymphoma cells. Therefore, these results provide preliminary evidence of the oncolytic activity of NDV in feline leukemia virus-induced lymphoma.https://www.frontiersin.org/articles/10.3389/fvets.2025.1484947/fullcatvirotherapyoncolyticNewcastle Disease Virusnew therapiescancer |
| spellingShingle | Talita Gabriela Luna Alves Pedro Luiz Porfirio Xavier Taismara Kustro Garnica Arina Lázaro Rochetti Talal Jamil Qazi Thiago Henrique Annibale Vendramini Felipe Haddad Muhammad Munir Márcio Antonio Brunetto Heidge Fukumasu Oncolytic potential of Newcastle Disease Virus in feline lymphoma cells: an in vitro evaluation Frontiers in Veterinary Science cat virotherapy oncolytic Newcastle Disease Virus new therapies cancer |
| title | Oncolytic potential of Newcastle Disease Virus in feline lymphoma cells: an in vitro evaluation |
| title_full | Oncolytic potential of Newcastle Disease Virus in feline lymphoma cells: an in vitro evaluation |
| title_fullStr | Oncolytic potential of Newcastle Disease Virus in feline lymphoma cells: an in vitro evaluation |
| title_full_unstemmed | Oncolytic potential of Newcastle Disease Virus in feline lymphoma cells: an in vitro evaluation |
| title_short | Oncolytic potential of Newcastle Disease Virus in feline lymphoma cells: an in vitro evaluation |
| title_sort | oncolytic potential of newcastle disease virus in feline lymphoma cells an in vitro evaluation |
| topic | cat virotherapy oncolytic Newcastle Disease Virus new therapies cancer |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1484947/full |
| work_keys_str_mv | AT talitagabrielalunaalves oncolyticpotentialofnewcastlediseasevirusinfelinelymphomacellsaninvitroevaluation AT pedroluizporfirioxavier oncolyticpotentialofnewcastlediseasevirusinfelinelymphomacellsaninvitroevaluation AT taismarakustrogarnica oncolyticpotentialofnewcastlediseasevirusinfelinelymphomacellsaninvitroevaluation AT arinalazarorochetti oncolyticpotentialofnewcastlediseasevirusinfelinelymphomacellsaninvitroevaluation AT talaljamilqazi oncolyticpotentialofnewcastlediseasevirusinfelinelymphomacellsaninvitroevaluation AT thiagohenriqueannibalevendramini oncolyticpotentialofnewcastlediseasevirusinfelinelymphomacellsaninvitroevaluation AT felipehaddad oncolyticpotentialofnewcastlediseasevirusinfelinelymphomacellsaninvitroevaluation AT muhammadmunir oncolyticpotentialofnewcastlediseasevirusinfelinelymphomacellsaninvitroevaluation AT marcioantoniobrunetto oncolyticpotentialofnewcastlediseasevirusinfelinelymphomacellsaninvitroevaluation AT heidgefukumasu oncolyticpotentialofnewcastlediseasevirusinfelinelymphomacellsaninvitroevaluation |