Genome-wide identification and expression profiling reveal the diverse role of Methyl-CpG-binding domain proteins in tea plant Camellia sinensis
Methyl-CpG-binding domain (MBD) proteins are important DNA methylation readers that recognise methylated CpG sites and recruit histone deacetylase (HDAC) complexes and chromatin remodelling factors, leading to chromatin compaction, gene transcription, and genome integrity. Currently, MBD genes have...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2021-03-01
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| Series: | Beverage Plant Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/BPR-2021-0010 |
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| author | Yao Chen Yuteng Li Hengze Ren Jianrong Zhou Lu Wang Yajun Yang Xinyuan Hao Xinchao Wang |
| author_facet | Yao Chen Yuteng Li Hengze Ren Jianrong Zhou Lu Wang Yajun Yang Xinyuan Hao Xinchao Wang |
| author_sort | Yao Chen |
| collection | DOAJ |
| description | Methyl-CpG-binding domain (MBD) proteins are important DNA methylation readers that recognise methylated CpG sites and recruit histone deacetylase (HDAC) complexes and chromatin remodelling factors, leading to chromatin compaction, gene transcription, and genome integrity. Currently, MBD genes have only been identified in a few plant species and their structure and function in tea plants (Camellia sinensis) are unknown. In this study, 16 C. sinensis MBD genes (CsMBD) were identified on a genome-wide level and classified into eight classes. The CsMBD genes were mapped on nine chromosomes in tea plants, and nine pairs of CsMBD genes existed. Based on conserved domain analysis, all of the identified CsMBD proteins contained at least one MBD domain. Expression analyses showed that CsMBD genes were expressed in tissue- and organ-specific patterns. We investigated the expression patterns of CsMBD genes in response to abiotic and biotic stresses and during different plant growth and development stages. Multiple pthytohormone and stress-related cis-acting was evaluated in their promoter region, such as GGTCA, TGACG, ABRE and LTR. Specific CsMBD genes were associated with environmental stresses and developmental stages, with little overlap. Overall, our findings reveal the diverse roles of CsMBD genes under different stress and developmental conditions, highlighting candidate genes for further functional studies on tea plants. |
| format | Article |
| id | doaj-art-9e34ef16c3094a6e92b6d07ac9b2d794 |
| institution | OA Journals |
| issn | 2769-2108 |
| language | English |
| publishDate | 2021-03-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Beverage Plant Research |
| spelling | doaj-art-9e34ef16c3094a6e92b6d07ac9b2d7942025-08-20T02:12:33ZengMaximum Academic PressBeverage Plant Research2769-21082021-03-011111210.48130/BPR-2021-0010BPR-2021-0010Genome-wide identification and expression profiling reveal the diverse role of Methyl-CpG-binding domain proteins in tea plant Camellia sinensisYao Chen0Yuteng Li1Hengze Ren2Jianrong Zhou3Lu Wang4Yajun Yang5Xinyuan Hao6Xinchao Wang7Tea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilisation, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilisation, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilisation, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilisation, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilisation, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilisation, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilisation, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilisation, Ministry of Agriculture and Rural Affairs, Hangzhou, Zhejiang 310008, ChinaMethyl-CpG-binding domain (MBD) proteins are important DNA methylation readers that recognise methylated CpG sites and recruit histone deacetylase (HDAC) complexes and chromatin remodelling factors, leading to chromatin compaction, gene transcription, and genome integrity. Currently, MBD genes have only been identified in a few plant species and their structure and function in tea plants (Camellia sinensis) are unknown. In this study, 16 C. sinensis MBD genes (CsMBD) were identified on a genome-wide level and classified into eight classes. The CsMBD genes were mapped on nine chromosomes in tea plants, and nine pairs of CsMBD genes existed. Based on conserved domain analysis, all of the identified CsMBD proteins contained at least one MBD domain. Expression analyses showed that CsMBD genes were expressed in tissue- and organ-specific patterns. We investigated the expression patterns of CsMBD genes in response to abiotic and biotic stresses and during different plant growth and development stages. Multiple pthytohormone and stress-related cis-acting was evaluated in their promoter region, such as GGTCA, TGACG, ABRE and LTR. Specific CsMBD genes were associated with environmental stresses and developmental stages, with little overlap. Overall, our findings reveal the diverse roles of CsMBD genes under different stress and developmental conditions, highlighting candidate genes for further functional studies on tea plants.https://www.maxapress.com/article/doi/10.48130/BPR-2021-0010dna methylationmethyl-cpg-binding domain proteinsgene familyexpression profilestea plant |
| spellingShingle | Yao Chen Yuteng Li Hengze Ren Jianrong Zhou Lu Wang Yajun Yang Xinyuan Hao Xinchao Wang Genome-wide identification and expression profiling reveal the diverse role of Methyl-CpG-binding domain proteins in tea plant Camellia sinensis Beverage Plant Research dna methylation methyl-cpg-binding domain proteins gene family expression profiles tea plant |
| title | Genome-wide identification and expression profiling reveal the diverse role of Methyl-CpG-binding domain proteins in tea plant Camellia sinensis |
| title_full | Genome-wide identification and expression profiling reveal the diverse role of Methyl-CpG-binding domain proteins in tea plant Camellia sinensis |
| title_fullStr | Genome-wide identification and expression profiling reveal the diverse role of Methyl-CpG-binding domain proteins in tea plant Camellia sinensis |
| title_full_unstemmed | Genome-wide identification and expression profiling reveal the diverse role of Methyl-CpG-binding domain proteins in tea plant Camellia sinensis |
| title_short | Genome-wide identification and expression profiling reveal the diverse role of Methyl-CpG-binding domain proteins in tea plant Camellia sinensis |
| title_sort | genome wide identification and expression profiling reveal the diverse role of methyl cpg binding domain proteins in tea plant camellia sinensis |
| topic | dna methylation methyl-cpg-binding domain proteins gene family expression profiles tea plant |
| url | https://www.maxapress.com/article/doi/10.48130/BPR-2021-0010 |
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