Comparative Study on Antioxidant Capacity of Diverse Food Matrices: Applicability, Suitability and Inter-Correlation of Multiple Assays to Assess Polyphenol and Antioxidant Status
Antioxidants play a crucial role in mitigating oxidative stress and preventing cellular damage caused by free radicals. This study aimed to compare the effectiveness of three antioxidant assays—DPPH, TEAC, and FRAP—in quantifying the antioxidant capacity of 15 plant-based spices, herbs, and food mat...
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MDPI AG
2025-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/3/317 |
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| author | Attila Kiss Vivien Anna Papp Anna Pál József Prokisch Sara Mirani Bela E. Toth Tarek Alshaal |
| author_facet | Attila Kiss Vivien Anna Papp Anna Pál József Prokisch Sara Mirani Bela E. Toth Tarek Alshaal |
| author_sort | Attila Kiss |
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| description | Antioxidants play a crucial role in mitigating oxidative stress and preventing cellular damage caused by free radicals. This study aimed to compare the effectiveness of three antioxidant assays—DPPH, TEAC, and FRAP—in quantifying the antioxidant capacity of 15 plant-based spices, herbs, and food materials from five distinct plant families. The relationship between these assays and total polyphenol content (TPC) as well as total flavonoid content (TFC) was also investigated. The results showed that FRAP exhibited the strongest correlation with TPC (r = 0.913), followed by TEAC (r = 0.856) and DPPH (r = 0.772). Lamiaceae species, such as rosemary and thyme, consistently demonstrated high antioxidant activities across all assays. The study highlights the complementary nature of these assays in assessing antioxidant capacity and underscores their utility in profiling polyphenol- and flavonoid-rich plants for potential nutritional and therapeutic applications. |
| format | Article |
| id | doaj-art-9e299660a7944e26ac3e78c261f7dc46 |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-9e299660a7944e26ac3e78c261f7dc462025-08-20T02:11:21ZengMDPI AGAntioxidants2076-39212025-03-0114331710.3390/antiox14030317Comparative Study on Antioxidant Capacity of Diverse Food Matrices: Applicability, Suitability and Inter-Correlation of Multiple Assays to Assess Polyphenol and Antioxidant StatusAttila Kiss0Vivien Anna Papp1Anna Pál2József Prokisch3Sara Mirani4Bela E. Toth5Tarek Alshaal6Agro- and Food-Industrial Research Centre, Széchenyi István University, Egyetem Sqr. 1, 9026 Győr, HungaryCentre for Agro- and Food-industrial Innovation, Faculty for Agro and Food Sciences, University of Debrecen, Böszörményi Str. 138, 4032 Debrecen, HungaryCentre for Agro- and Food-industrial Innovation, Faculty for Agro and Food Sciences, University of Debrecen, Böszörményi Str. 138, 4032 Debrecen, HungaryDepartment of Animal Husbandry, Faculty for Agro and Food Sciences, University of Debrecen, Böszörményi Str. 138, 4032 Debrecen, HungaryDepartment of Pharmaceutical Surveillance and Economics, Faculty of Pharmacy, University of Debrecen, Egyetem Sqr. 1, 4032 Debrecen, HungaryDepartment of Pharmaceutical Surveillance and Economics, Faculty of Pharmacy, University of Debrecen, Egyetem Sqr. 1, 4032 Debrecen, HungaryDepartment of Applied Plant Biology, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi Str. 138, 4032 Debrecen, HungaryAntioxidants play a crucial role in mitigating oxidative stress and preventing cellular damage caused by free radicals. This study aimed to compare the effectiveness of three antioxidant assays—DPPH, TEAC, and FRAP—in quantifying the antioxidant capacity of 15 plant-based spices, herbs, and food materials from five distinct plant families. The relationship between these assays and total polyphenol content (TPC) as well as total flavonoid content (TFC) was also investigated. The results showed that FRAP exhibited the strongest correlation with TPC (r = 0.913), followed by TEAC (r = 0.856) and DPPH (r = 0.772). Lamiaceae species, such as rosemary and thyme, consistently demonstrated high antioxidant activities across all assays. The study highlights the complementary nature of these assays in assessing antioxidant capacity and underscores their utility in profiling polyphenol- and flavonoid-rich plants for potential nutritional and therapeutic applications.https://www.mdpi.com/2076-3921/14/3/317bioactive compoundsradical scavenging activityreducing powernutritional profilinghealth-promoting propertiescomparative bioactivity |
| spellingShingle | Attila Kiss Vivien Anna Papp Anna Pál József Prokisch Sara Mirani Bela E. Toth Tarek Alshaal Comparative Study on Antioxidant Capacity of Diverse Food Matrices: Applicability, Suitability and Inter-Correlation of Multiple Assays to Assess Polyphenol and Antioxidant Status Antioxidants bioactive compounds radical scavenging activity reducing power nutritional profiling health-promoting properties comparative bioactivity |
| title | Comparative Study on Antioxidant Capacity of Diverse Food Matrices: Applicability, Suitability and Inter-Correlation of Multiple Assays to Assess Polyphenol and Antioxidant Status |
| title_full | Comparative Study on Antioxidant Capacity of Diverse Food Matrices: Applicability, Suitability and Inter-Correlation of Multiple Assays to Assess Polyphenol and Antioxidant Status |
| title_fullStr | Comparative Study on Antioxidant Capacity of Diverse Food Matrices: Applicability, Suitability and Inter-Correlation of Multiple Assays to Assess Polyphenol and Antioxidant Status |
| title_full_unstemmed | Comparative Study on Antioxidant Capacity of Diverse Food Matrices: Applicability, Suitability and Inter-Correlation of Multiple Assays to Assess Polyphenol and Antioxidant Status |
| title_short | Comparative Study on Antioxidant Capacity of Diverse Food Matrices: Applicability, Suitability and Inter-Correlation of Multiple Assays to Assess Polyphenol and Antioxidant Status |
| title_sort | comparative study on antioxidant capacity of diverse food matrices applicability suitability and inter correlation of multiple assays to assess polyphenol and antioxidant status |
| topic | bioactive compounds radical scavenging activity reducing power nutritional profiling health-promoting properties comparative bioactivity |
| url | https://www.mdpi.com/2076-3921/14/3/317 |
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