Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells

Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells

Abstract Background Lumpy skin disease (LSD), caused by infection with the lumpy skin disease virus (LSDV), is a highly infectious disease that poses a notable challenge to the cattle industry worldwide. To conduct epidemiological monitoring of LSDV infection in cattle and evaluate the immune effica...

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Bibliographic Details
Main Authors: Ruolan Xin, Jiawen Zhang, Yinghui Zhang, Daoming Su, Menglin Li, Dan Liu, Ruizhi Wu, Junjie Zhao, Yuanyuan Zhu, Yebing Liu, Xiaoyun Chen, Yongjun Wen, Zhen Zhu
Format: Article
Language:English
Published: BMC 2025-06-01
Series:BMC Biotechnology
Subjects:
Lumpy skin disease virus
Indirect ELISA
CHO-K1 cells
P32 protein
Online Access:https://doi.org/10.1186/s12896-025-00966-6
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