Effects of interaction between ectomycorrhizal fungal and mycorrhiza helper bacteria on Picea abies seedlings growth
Early growth and establishment success of Picea abies is poor, so ectomycorrhizal (ECM) fungi can be used to promote survival, establishment, as well as the growth of seedlings. This study aimed to evaluate the effect of valuable ECM fungi symbiosis and mycorrhiza helper bacteria (Bacillus cereus) o...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Guilan
2023-07-01
|
| Series: | Caspian Journal of Environmental Sciences |
| Subjects: | |
| Online Access: | https://cjes.guilan.ac.ir/article_6960_25824e7d4e3beb8aa36b3928850709cd.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850121906532909056 |
|---|---|
| author | Sheno Amini Moazzam Hassanpour Asil Jamalali Olfati Sedigheh Mousanejad |
| author_facet | Sheno Amini Moazzam Hassanpour Asil Jamalali Olfati Sedigheh Mousanejad |
| author_sort | Sheno Amini |
| collection | DOAJ |
| description | Early growth and establishment success of Picea abies is poor, so ectomycorrhizal (ECM) fungi can be used to promote survival, establishment, as well as the growth of seedlings. This study aimed to evaluate the effect of valuable ECM fungi symbiosis and mycorrhiza helper bacteria (Bacillus cereus) on the growth of P. abies. The treatments included B. cereus, ECM fungi of Cantharellus cibarius, Amanita caesarea, Boletus edulis, three combined treatments (Bacillus + ECM fungi), and control. 2-month-old seedlings were inoculated with fungi and bacteria. Then root mycorrhization percent, morphological traits, chlorophyll content and nutrient uptake were evaluated in 15 months after plant inoculation. The results indicated that mycorrhization was significantly influenced by applied treatments. The highest (50.33%) and lowest (27.67%) mycorrhizations were obtained by B. edulis + B. cereus and C. cibarius, respectively. The combination treatments dramatically increased the plant height, stem and root dry weight, total chlorophyll, absorption of potassium and nitrogen compared to the control. The combined inoculation with B. cereus + B. edulis also resulted in an increase in K and Ca uptake as well as chlorophyll a compared to control. The lowest values for all evaluated traits except Ca uptake were obtained by control. Moreover, the highest values for all traits were observed in plants co-inoculated with B. cereus + B. edulis. In conclusion, the results suggested that the ECM fungi, especially Boletus, had a symbiosis with Norway spruce. The symbiosis was also improved by B. cereus. |
| format | Article |
| id | doaj-art-9e1cec93b80b4a069aa33ffdcb954e0a |
| institution | OA Journals |
| issn | 1735-3033 1735-3866 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | University of Guilan |
| record_format | Article |
| series | Caspian Journal of Environmental Sciences |
| spelling | doaj-art-9e1cec93b80b4a069aa33ffdcb954e0a2025-08-20T02:34:57ZengUniversity of GuilanCaspian Journal of Environmental Sciences1735-30331735-38662023-07-0121373774710.22124/cjes.2023.69606960Effects of interaction between ectomycorrhizal fungal and mycorrhiza helper bacteria on Picea abies seedlings growthSheno Amini0Moazzam Hassanpour Asil1Jamalali Olfati2Sedigheh Mousanejad3Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDepartment of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDepartment of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDepartment of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranEarly growth and establishment success of Picea abies is poor, so ectomycorrhizal (ECM) fungi can be used to promote survival, establishment, as well as the growth of seedlings. This study aimed to evaluate the effect of valuable ECM fungi symbiosis and mycorrhiza helper bacteria (Bacillus cereus) on the growth of P. abies. The treatments included B. cereus, ECM fungi of Cantharellus cibarius, Amanita caesarea, Boletus edulis, three combined treatments (Bacillus + ECM fungi), and control. 2-month-old seedlings were inoculated with fungi and bacteria. Then root mycorrhization percent, morphological traits, chlorophyll content and nutrient uptake were evaluated in 15 months after plant inoculation. The results indicated that mycorrhization was significantly influenced by applied treatments. The highest (50.33%) and lowest (27.67%) mycorrhizations were obtained by B. edulis + B. cereus and C. cibarius, respectively. The combination treatments dramatically increased the plant height, stem and root dry weight, total chlorophyll, absorption of potassium and nitrogen compared to the control. The combined inoculation with B. cereus + B. edulis also resulted in an increase in K and Ca uptake as well as chlorophyll a compared to control. The lowest values for all evaluated traits except Ca uptake were obtained by control. Moreover, the highest values for all traits were observed in plants co-inoculated with B. cereus + B. edulis. In conclusion, the results suggested that the ECM fungi, especially Boletus, had a symbiosis with Norway spruce. The symbiosis was also improved by B. cereus.https://cjes.guilan.ac.ir/article_6960_25824e7d4e3beb8aa36b3928850709cd.pdfcolonizationgrowthmycorrhizal funginorway sprucenutrient absorption |
| spellingShingle | Sheno Amini Moazzam Hassanpour Asil Jamalali Olfati Sedigheh Mousanejad Effects of interaction between ectomycorrhizal fungal and mycorrhiza helper bacteria on Picea abies seedlings growth Caspian Journal of Environmental Sciences colonization growth mycorrhizal fungi norway spruce nutrient absorption |
| title | Effects of interaction between ectomycorrhizal fungal and mycorrhiza helper bacteria on Picea abies seedlings growth |
| title_full | Effects of interaction between ectomycorrhizal fungal and mycorrhiza helper bacteria on Picea abies seedlings growth |
| title_fullStr | Effects of interaction between ectomycorrhizal fungal and mycorrhiza helper bacteria on Picea abies seedlings growth |
| title_full_unstemmed | Effects of interaction between ectomycorrhizal fungal and mycorrhiza helper bacteria on Picea abies seedlings growth |
| title_short | Effects of interaction between ectomycorrhizal fungal and mycorrhiza helper bacteria on Picea abies seedlings growth |
| title_sort | effects of interaction between ectomycorrhizal fungal and mycorrhiza helper bacteria on picea abies seedlings growth |
| topic | colonization growth mycorrhizal fungi norway spruce nutrient absorption |
| url | https://cjes.guilan.ac.ir/article_6960_25824e7d4e3beb8aa36b3928850709cd.pdf |
| work_keys_str_mv | AT shenoamini effectsofinteractionbetweenectomycorrhizalfungalandmycorrhizahelperbacteriaonpiceaabiesseedlingsgrowth AT moazzamhassanpourasil effectsofinteractionbetweenectomycorrhizalfungalandmycorrhizahelperbacteriaonpiceaabiesseedlingsgrowth AT jamalaliolfati effectsofinteractionbetweenectomycorrhizalfungalandmycorrhizahelperbacteriaonpiceaabiesseedlingsgrowth AT sedighehmousanejad effectsofinteractionbetweenectomycorrhizalfungalandmycorrhizahelperbacteriaonpiceaabiesseedlingsgrowth |