GC-MS metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two Chinese rose cultivars
Floral fragrance is an important ornamental trait of horticultural crops. However, research on the physiological and molecular biology of the floral volatile compounds in Chinese rose is limited. This study conducted metabolomic and transcriptomic analyses on the floral volatile compounds of Rosa ch...
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| Language: | English |
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Maximum Academic Press
2025-01-01
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| Series: | Ornamental Plant Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/opr-0025-0003 |
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| author | Kaiqing Luo Chao Song Tingting Bao Rui Huang Shengnan Lin Yingxue Yang Luhong Leng Xuezhu Liao Cuihua Gu Zhiqiang Wu Xiaoni Zhang |
| author_facet | Kaiqing Luo Chao Song Tingting Bao Rui Huang Shengnan Lin Yingxue Yang Luhong Leng Xuezhu Liao Cuihua Gu Zhiqiang Wu Xiaoni Zhang |
| author_sort | Kaiqing Luo |
| collection | DOAJ |
| description | Floral fragrance is an important ornamental trait of horticultural crops. However, research on the physiological and molecular biology of the floral volatile compounds in Chinese rose is limited. This study conducted metabolomic and transcriptomic analyses on the floral volatile compounds of Rosa chinensis (R. chinensis) 'Old Blush' ('OB') and R. chinensis 'Chilong Hanzhu' ('CH'). A total of 22 major volatile organic compounds (VOCs) were detected in the blooming flowers of 'OB' and 'CH'. The main VOCs emitted from 'OB' petals was 1,3,5-trimethoxybenzene (TMB), while that from 'CH' petals was 2-phenylethanol (2-PE). The GO and KEGG enrichment on differentially expressed genes (DEGs) revealed the essential role of phenylpropanoid pathway in the difference of floral fragrance between two cultivars, and a total of 15 key genes involved in the biosynthesis of phenylpropanoid were identified in R. chinensis flowers. The overexpression of RcCH_AADC-1 in transgenic tobacco plants led to the accumulation of 2-PE. In contrast, no significant change in the level of 2-PE was observed after the transient transformation of RcCH_PAR1 or RcCH_PAR1_Like into tobacco plants. These findings provide valuable insight for enhancing the accumulation of 2-PE in R. chinensis. |
| format | Article |
| id | doaj-art-9e14dedff7bb4ea1adabd42dcb8a3393 |
| institution | DOAJ |
| issn | 2769-2094 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Ornamental Plant Research |
| spelling | doaj-art-9e14dedff7bb4ea1adabd42dcb8a33932025-08-20T03:18:35ZengMaximum Academic PressOrnamental Plant Research2769-20942025-01-015111010.48130/opr-0025-0003opr-0025-0003GC-MS metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two Chinese rose cultivarsKaiqing Luo0Chao Song1Tingting Bao2Rui Huang3Shengnan Lin4Yingxue Yang5Luhong Leng6Xuezhu Liao7Cuihua Gu8Zhiqiang Wu9Xiaoni Zhang10Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaCollege of Landscape and Architecture, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, ChinaFloral fragrance is an important ornamental trait of horticultural crops. However, research on the physiological and molecular biology of the floral volatile compounds in Chinese rose is limited. This study conducted metabolomic and transcriptomic analyses on the floral volatile compounds of Rosa chinensis (R. chinensis) 'Old Blush' ('OB') and R. chinensis 'Chilong Hanzhu' ('CH'). A total of 22 major volatile organic compounds (VOCs) were detected in the blooming flowers of 'OB' and 'CH'. The main VOCs emitted from 'OB' petals was 1,3,5-trimethoxybenzene (TMB), while that from 'CH' petals was 2-phenylethanol (2-PE). The GO and KEGG enrichment on differentially expressed genes (DEGs) revealed the essential role of phenylpropanoid pathway in the difference of floral fragrance between two cultivars, and a total of 15 key genes involved in the biosynthesis of phenylpropanoid were identified in R. chinensis flowers. The overexpression of RcCH_AADC-1 in transgenic tobacco plants led to the accumulation of 2-PE. In contrast, no significant change in the level of 2-PE was observed after the transient transformation of RcCH_PAR1 or RcCH_PAR1_Like into tobacco plants. These findings provide valuable insight for enhancing the accumulation of 2-PE in R. chinensis.https://www.maxapress.com/article/doi/10.48130/opr-0025-0003rosa chinensisvolatile organic compounds (vocs)phenylpropanoid pathwaygenes2-pe |
| spellingShingle | Kaiqing Luo Chao Song Tingting Bao Rui Huang Shengnan Lin Yingxue Yang Luhong Leng Xuezhu Liao Cuihua Gu Zhiqiang Wu Xiaoni Zhang GC-MS metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two Chinese rose cultivars Ornamental Plant Research rosa chinensis volatile organic compounds (vocs) phenylpropanoid pathway genes 2-pe |
| title | GC-MS metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two Chinese rose cultivars |
| title_full | GC-MS metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two Chinese rose cultivars |
| title_fullStr | GC-MS metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two Chinese rose cultivars |
| title_full_unstemmed | GC-MS metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two Chinese rose cultivars |
| title_short | GC-MS metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two Chinese rose cultivars |
| title_sort | gc ms metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two chinese rose cultivars |
| topic | rosa chinensis volatile organic compounds (vocs) phenylpropanoid pathway genes 2-pe |
| url | https://www.maxapress.com/article/doi/10.48130/opr-0025-0003 |
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