GC-MS metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two Chinese rose cultivars

GC-MS metabolite and transcriptome analyses reveal the differences of volatile synthesis and gene expression profiling between two Chinese rose cultivars

Floral fragrance is an important ornamental trait of horticultural crops. However, research on the physiological and molecular biology of the floral volatile compounds in Chinese rose is limited. This study conducted metabolomic and transcriptomic analyses on the floral volatile compounds of Rosa ch...

Full description

Saved in:
Bibliographic Details
Main Authors: Kaiqing Luo, Chao Song, Tingting Bao, Rui Huang, Shengnan Lin, Yingxue Yang, Luhong Leng, Xuezhu Liao, Cuihua Gu, Zhiqiang Wu, Xiaoni Zhang
Format: Article
Language:English
Published: Maximum Academic Press 2025-01-01
Series:Ornamental Plant Research
Subjects:
rosa chinensis
volatile organic compounds (vocs)
phenylpropanoid pathway
genes
2-pe
Online Access:https://www.maxapress.com/article/doi/10.48130/opr-0025-0003
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Floral fragrance is an important ornamental trait of horticultural crops. However, research on the physiological and molecular biology of the floral volatile compounds in Chinese rose is limited. This study conducted metabolomic and transcriptomic analyses on the floral volatile compounds of Rosa chinensis (R. chinensis) 'Old Blush' ('OB') and R. chinensis 'Chilong Hanzhu' ('CH'). A total of 22 major volatile organic compounds (VOCs) were detected in the blooming flowers of 'OB' and 'CH'. The main VOCs emitted from 'OB' petals was 1,3,5-trimethoxybenzene (TMB), while that from 'CH' petals was 2-phenylethanol (2-PE). The GO and KEGG enrichment on differentially expressed genes (DEGs) revealed the essential role of phenylpropanoid pathway in the difference of floral fragrance between two cultivars, and a total of 15 key genes involved in the biosynthesis of phenylpropanoid were identified in R. chinensis flowers. The overexpression of RcCH_AADC-1 in transgenic tobacco plants led to the accumulation of 2-PE. In contrast, no significant change in the level of 2-PE was observed after the transient transformation of RcCH_PAR1 or RcCH_PAR1_Like into tobacco plants. These findings provide valuable insight for enhancing the accumulation of 2-PE in R. chinensis.
ISSN:2769-2094

Similar Items