In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia
This study was conducted to develop an efficient regeneration protocol used for sesame genetic transformation. Published regeneration methods using 6-benzylaminopurine (BAP), indol-3-butyric acid (IBA), and α-naphthaleneacetic acid (NAA) were used in this experiment. Cotyledon explants of 14 Ethiopi...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Agronomy |
| Online Access: | http://dx.doi.org/10.1155/2020/8878104 |
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| author | Micheale Yifter Weldemichael Yemane Tsehaye Bayratsion Desta Berhe Sbhatu Girmay Gebresamuel Abraha Hagos Mohammedseid Juhar Abraha Birhan Kassa Fiseha Baraki Sibhatu Birhanu Debesay Berhe Tagel Elias Sahle Mohammed Mebrahtu Mossa Haftay Abadi Gebru |
| author_facet | Micheale Yifter Weldemichael Yemane Tsehaye Bayratsion Desta Berhe Sbhatu Girmay Gebresamuel Abraha Hagos Mohammedseid Juhar Abraha Birhan Kassa Fiseha Baraki Sibhatu Birhanu Debesay Berhe Tagel Elias Sahle Mohammed Mebrahtu Mossa Haftay Abadi Gebru |
| author_sort | Micheale Yifter Weldemichael |
| collection | DOAJ |
| description | This study was conducted to develop an efficient regeneration protocol used for sesame genetic transformation. Published regeneration methods using 6-benzylaminopurine (BAP), indol-3-butyric acid (IBA), and α-naphthaleneacetic acid (NAA) were used in this experiment. Cotyledon explants of 14 Ethiopian genotypes collected from Humera Agricultural Research Center (HuARC) were used. Optimized culture conditions were used to investigate the regeneration response of different genotypes. Significant interactions between hormone treatments, MS media, and genotypes for shoot and root regeneration were recorded. Results showed that culture of cotyledons were developed and used as a source of explants for shoot regeneration. The highest shoot number, leaf number, and shoot length were recorded on explants cultured on 1.00 mg·L−1 BAP + 1.00 mg·L−1 NAA in Hirhir and Aberghele, 0.75 mg·L−1 BAP + 1.00 mg·L−1 NAA in Baha Zeyit, and 1.0 mg·L−1 BAP + 1.00 mg·L−1 NAA in Humera 1, respectively. The lowest shoot number, leaf number, and shoot length were observed on explants cultured on the control in Gondar 1, Borkana, and Baha Necho, Borkana and Beha Necho, and Baha Necho, respectively. Genotypes with well-developed shoots were transferred to root induction media. Under rooting media, the best mean, root number, and root length were observed in Aberghele and ACC44 at 0.1 mg·L−1 IBA and 0.2 mg·L−1 NAA, respectively. Standardizing genotypes trait data to mean 0 and unity variance has helped to group 14 genotypes into four distinct clusters. Hirhir, Abeghele, Baha Zeyit, and Humera 1 were the best genotypes. These findings lay ground to Ethiopian sesame to do further genetic transformation aiming at improving the crop. |
| format | Article |
| id | doaj-art-9e1148dae9bb4a1aa3f5a9e85871b3a8 |
| institution | OA Journals |
| issn | 1687-8159 1687-8167 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
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| series | International Journal of Agronomy |
| spelling | doaj-art-9e1148dae9bb4a1aa3f5a9e85871b3a82025-08-20T02:20:29ZengWileyInternational Journal of Agronomy1687-81591687-81672020-01-01202010.1155/2020/88781048878104In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in EthiopiaMicheale Yifter Weldemichael0Yemane Tsehaye Bayratsion1Desta Berhe Sbhatu2Girmay Gebresamuel Abraha3Hagos Mohammedseid Juhar4Abraha Birhan Kassa5Fiseha Baraki Sibhatu6Birhanu Debesay Berhe7Tagel Elias Sahle8Mohammed Mebrahtu Mossa9Haftay Abadi Gebru10Department of Biotechnology, CDANR, Mekelle University, P.O. Box 231, Mekelle, EthiopiaDepartment of DCHS, CDANR, Mekelle University, P.O. Box 231, Mekelle, EthiopiaDepartment of BCEN, MIT, Mekelle University, P.O. Box 231, Mekelle, EthiopiaDepartment of LaRMEP, CDANR, Mekelle University, P.O. Box 231, Mekelle, EthiopiaDepartment of Biotechnology, CDANR, Mekelle University, P.O. Box 231, Mekelle, EthiopiaDepartment of Chemistry, CDANR, Mekelle University, P.O. Box 231, Mekelle, EthiopiaTigrai Agricultural Research Institute, HuARC, P.O. Box 510, Humera, EthiopiaTigrai Biotechnology Center Pvt. Ltd. Co., P.O. Box 223, Mekelle, EthiopiaDilla University, P.O. Box 419, Dilla, EthiopiaDepartment of DCHS, CDANR, Mekelle University, P.O. Box 231, Mekelle, EthiopiaTigrai Biotechnology Center Pvt. Ltd. Co., P.O. Box 223, Mekelle, EthiopiaThis study was conducted to develop an efficient regeneration protocol used for sesame genetic transformation. Published regeneration methods using 6-benzylaminopurine (BAP), indol-3-butyric acid (IBA), and α-naphthaleneacetic acid (NAA) were used in this experiment. Cotyledon explants of 14 Ethiopian genotypes collected from Humera Agricultural Research Center (HuARC) were used. Optimized culture conditions were used to investigate the regeneration response of different genotypes. Significant interactions between hormone treatments, MS media, and genotypes for shoot and root regeneration were recorded. Results showed that culture of cotyledons were developed and used as a source of explants for shoot regeneration. The highest shoot number, leaf number, and shoot length were recorded on explants cultured on 1.00 mg·L−1 BAP + 1.00 mg·L−1 NAA in Hirhir and Aberghele, 0.75 mg·L−1 BAP + 1.00 mg·L−1 NAA in Baha Zeyit, and 1.0 mg·L−1 BAP + 1.00 mg·L−1 NAA in Humera 1, respectively. The lowest shoot number, leaf number, and shoot length were observed on explants cultured on the control in Gondar 1, Borkana, and Baha Necho, Borkana and Beha Necho, and Baha Necho, respectively. Genotypes with well-developed shoots were transferred to root induction media. Under rooting media, the best mean, root number, and root length were observed in Aberghele and ACC44 at 0.1 mg·L−1 IBA and 0.2 mg·L−1 NAA, respectively. Standardizing genotypes trait data to mean 0 and unity variance has helped to group 14 genotypes into four distinct clusters. Hirhir, Abeghele, Baha Zeyit, and Humera 1 were the best genotypes. These findings lay ground to Ethiopian sesame to do further genetic transformation aiming at improving the crop.http://dx.doi.org/10.1155/2020/8878104 |
| spellingShingle | Micheale Yifter Weldemichael Yemane Tsehaye Bayratsion Desta Berhe Sbhatu Girmay Gebresamuel Abraha Hagos Mohammedseid Juhar Abraha Birhan Kassa Fiseha Baraki Sibhatu Birhanu Debesay Berhe Tagel Elias Sahle Mohammed Mebrahtu Mossa Haftay Abadi Gebru In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia International Journal of Agronomy |
| title | In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia |
| title_full | In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia |
| title_fullStr | In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia |
| title_full_unstemmed | In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia |
| title_short | In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia |
| title_sort | in vitro shoot regeneration of oil seed crop sesamum indicum l from seedling cotyledon explant to lay ground for genetic transformation in ethiopia |
| url | http://dx.doi.org/10.1155/2020/8878104 |
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