miR-1236-3p targets Toll-like receptor 4 to suppress the anti-Mycobacterium tuberculosis activity of macrophage
Summary: Mycobacterium tuberculosis (Mtb) modulates host innate immunity via Toll-like receptor 4 (TLR4), associated with the susceptibility to Mtb. Bioinformatics predicted miR-1236-3p could be a potential target for the 3′-UTR of the TLR4 gene. However, the clinical significance and underlying mec...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225007837 |
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| author | Yating Zhang Jie Han Qianwei Yang Xiaogang Cui Huiping Duan Ting Wu Changxin Wu Li Xing Qunqun Liu Li Dong |
| author_facet | Yating Zhang Jie Han Qianwei Yang Xiaogang Cui Huiping Duan Ting Wu Changxin Wu Li Xing Qunqun Liu Li Dong |
| author_sort | Yating Zhang |
| collection | DOAJ |
| description | Summary: Mycobacterium tuberculosis (Mtb) modulates host innate immunity via Toll-like receptor 4 (TLR4), associated with the susceptibility to Mtb. Bioinformatics predicted miR-1236-3p could be a potential target for the 3′-UTR of the TLR4 gene. However, the clinical significance and underlying mechanisms remain unclear. To validate this, we analyzed miR-1236-3p levels in 81 subjects and observed that both active tuberculosis (ATB) and latent tuberculosis infection (LTBI) patients exhibited elevated miR-1236-3p levels compared to healthy control (HC) subjects. In vitro dual-luciferase reporter assays confirmed that miR-1236-3p specifically targeted the 3′-UTR of TLR4 mRNA. During Mtb infection in macrophages, miR-1236-3p enhanced the NF-κB signaling and reduced the release of intracellular inflammatory factors, reactive oxygen species, and nitric oxide (NO), indicating that the ability of macrophages to constrain intracellular Mtb infection was compromised by miR-1236-3p. In summary, miR-1236-3p may target TLR4/NF-κB signaling to suppress the intrinsic anti-Mtb activity of macrophage. |
| format | Article |
| id | doaj-art-9e0778951bc1460ab379d7fbc032ce74 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-9e0778951bc1460ab379d7fbc032ce742025-08-20T03:48:19ZengElsevieriScience2589-00422025-06-0128611252210.1016/j.isci.2025.112522miR-1236-3p targets Toll-like receptor 4 to suppress the anti-Mycobacterium tuberculosis activity of macrophageYating Zhang0Jie Han1Qianwei Yang2Xiaogang Cui3Huiping Duan4Ting Wu5Changxin Wu6Li Xing7Qunqun Liu8Li Dong9Institutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi Province 030006, ChinaInstitutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi Province 030006, ChinaInstitutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi Province 030006, ChinaInstitutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi Province 030006, ChinaThe Fourth People’s Hospital of Taiyuan, 231 Xikuang Street, Taiyuan 030006, ChinaThe Fourth People’s Hospital of Taiyuan, 231 Xikuang Street, Taiyuan 030006, ChinaInstitutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi Province 030006, ChinaInstitutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi Province 030006, China; Corresponding authorThe Fourth People’s Hospital of Taiyuan, 231 Xikuang Street, Taiyuan 030006, China; Corresponding authorInstitutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi Province 030006, China; Shanxi Provincial Key Laboratory of Medical Molecular Cell Biology, Shanxi University, 92 Wucheng Road, Taiyuan 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China; Corresponding authorSummary: Mycobacterium tuberculosis (Mtb) modulates host innate immunity via Toll-like receptor 4 (TLR4), associated with the susceptibility to Mtb. Bioinformatics predicted miR-1236-3p could be a potential target for the 3′-UTR of the TLR4 gene. However, the clinical significance and underlying mechanisms remain unclear. To validate this, we analyzed miR-1236-3p levels in 81 subjects and observed that both active tuberculosis (ATB) and latent tuberculosis infection (LTBI) patients exhibited elevated miR-1236-3p levels compared to healthy control (HC) subjects. In vitro dual-luciferase reporter assays confirmed that miR-1236-3p specifically targeted the 3′-UTR of TLR4 mRNA. During Mtb infection in macrophages, miR-1236-3p enhanced the NF-κB signaling and reduced the release of intracellular inflammatory factors, reactive oxygen species, and nitric oxide (NO), indicating that the ability of macrophages to constrain intracellular Mtb infection was compromised by miR-1236-3p. In summary, miR-1236-3p may target TLR4/NF-κB signaling to suppress the intrinsic anti-Mtb activity of macrophage.http://www.sciencedirect.com/science/article/pii/S2589004225007837ImmunologyImmune responseMicrobiology |
| spellingShingle | Yating Zhang Jie Han Qianwei Yang Xiaogang Cui Huiping Duan Ting Wu Changxin Wu Li Xing Qunqun Liu Li Dong miR-1236-3p targets Toll-like receptor 4 to suppress the anti-Mycobacterium tuberculosis activity of macrophage iScience Immunology Immune response Microbiology |
| title | miR-1236-3p targets Toll-like receptor 4 to suppress the anti-Mycobacterium tuberculosis activity of macrophage |
| title_full | miR-1236-3p targets Toll-like receptor 4 to suppress the anti-Mycobacterium tuberculosis activity of macrophage |
| title_fullStr | miR-1236-3p targets Toll-like receptor 4 to suppress the anti-Mycobacterium tuberculosis activity of macrophage |
| title_full_unstemmed | miR-1236-3p targets Toll-like receptor 4 to suppress the anti-Mycobacterium tuberculosis activity of macrophage |
| title_short | miR-1236-3p targets Toll-like receptor 4 to suppress the anti-Mycobacterium tuberculosis activity of macrophage |
| title_sort | mir 1236 3p targets toll like receptor 4 to suppress the anti mycobacterium tuberculosis activity of macrophage |
| topic | Immunology Immune response Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225007837 |
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