EasyMetagenome: A user‐friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

Abstract Shotgun metagenomics has become a pivotal technology in microbiome research, enabling in‐depth analysis of microbial communities at both the high‐resolution taxonomic and functional levels. This approach provides valuable insights of microbial diversity, interactions, and their roles in hea...

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Main Authors: Defeng Bai, Tong Chen, Jiani Xun, Chuang Ma, Hao Luo, Haifei Yang, Chen Cao, Xiaofeng Cao, Jianzhou Cui, Yuan‐Ping Deng, Zhaochao Deng, Wenxin Dong, Wenxue Dong, Juan Du, Qunkai Fang, Wei Fang, Yue Fang, Fangtian Fu, Min Fu, Yi‐Tian Fu, He Gao, Jingping Ge, Qinglong Gong, Lunda Gu, Peng Guo, Yuhao Guo, Tang Hai, Hao Liu, Jieqiang He, Zi‐Yang He, Huiyu Hou, Can Huang, Shuai Ji, ChangHai Jiang, Gui‐Lai Jiang, Lingjuan Jiang, Ling N. Jin, Yuhe Kan, Da Kang, Jin Kou, Ka‐Lung Lam, Changchao Li, Chong Li, Fuyi Li, Liwei Li, Miao Li, Xin Li, Ye Li, Zheng‐Tao Li, Jing Liang, Yongxin Lin, Changzhen Liu, Danni Liu, Fengqin Liu, Jia Liu, Tianrui Liu, Tingting Liu, Xinyuan Liu, Yaqun Liu, Bangyan Liu, Minghao Liu, Wenbo Lou, Yaning Luan, Yuanyuan Luo, Hujie Lv, Tengfei Ma, Zongjiong Mai, Jiayuan Mo, Dongze Niu, Zhuo Pan, Heyuan Qi, Zhanyao Shi, Chunjiao Song, Fuxiang Sun, Yan Sun, Sihui Tian, Xiulin Wan, Guoliang Wang, Hongyang Wang, Hongyu Wang, Huanhuan Wang, Jing Wang, Jun Wang, Kang Wang, Leli Wang, Shao‐kun Wang, Xinlong Wang, Yao Wang, Zufei Xiao, Huichun Xing, Yifan Xu, Shu‐yan Yan, Li Yang, Song Yang, Yuanming Yang, Xiaofang Yao, Salsabeel Yousuf, Hao Yu, Yu Lei, Zhengrong Yuan, Meiyin Zeng, Chunfang Zhang, Chunge Zhang, Huimin Zhang, Jing Zhang, Na Zhang, Tianyuan Zhang, Yi‐Bo Zhang, Yupeng Zhang, Zheng Zhang, Mingda Zhou, Yuanping Zhou, Chengshuai Zhu, Lin Zhu, Yue Zhu, Zhihao Zhu, Hongqin Zou, Anna Zuo, Wenxuan Dong, Tao Wen, Shifu Chen, Guoliang Li, Yunyun Gao, Yong‐Xin Liu
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Language:English
Published: Wiley 2025-02-01
Series:iMeta
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Online Access:https://doi.org/10.1002/imt2.70001
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author Defeng Bai
Tong Chen
Jiani Xun
Chuang Ma
Hao Luo
Haifei Yang
Chen Cao
Xiaofeng Cao
Jianzhou Cui
Yuan‐Ping Deng
Zhaochao Deng
Wenxin Dong
Wenxue Dong
Juan Du
Qunkai Fang
Wei Fang
Yue Fang
Fangtian Fu
Min Fu
Yi‐Tian Fu
He Gao
Jingping Ge
Qinglong Gong
Lunda Gu
Peng Guo
Yuhao Guo
Tang Hai
Hao Liu
Jieqiang He
Zi‐Yang He
Huiyu Hou
Can Huang
Shuai Ji
ChangHai Jiang
Gui‐Lai Jiang
Lingjuan Jiang
Ling N. Jin
Yuhe Kan
Da Kang
Jin Kou
Ka‐Lung Lam
Changchao Li
Chong Li
Fuyi Li
Liwei Li
Miao Li
Xin Li
Ye Li
Zheng‐Tao Li
Jing Liang
Yongxin Lin
Changzhen Liu
Danni Liu
Fengqin Liu
Jia Liu
Tianrui Liu
Tingting Liu
Xinyuan Liu
Yaqun Liu
Bangyan Liu
Minghao Liu
Wenbo Lou
Yaning Luan
Yuanyuan Luo
Hujie Lv
Tengfei Ma
Zongjiong Mai
Jiayuan Mo
Dongze Niu
Zhuo Pan
Heyuan Qi
Zhanyao Shi
Chunjiao Song
Fuxiang Sun
Yan Sun
Sihui Tian
Xiulin Wan
Guoliang Wang
Hongyang Wang
Hongyu Wang
Huanhuan Wang
Jing Wang
Jun Wang
Kang Wang
Leli Wang
Shao‐kun Wang
Xinlong Wang
Yao Wang
Zufei Xiao
Huichun Xing
Yifan Xu
Shu‐yan Yan
Li Yang
Song Yang
Yuanming Yang
Xiaofang Yao
Salsabeel Yousuf
Hao Yu
Yu Lei
Zhengrong Yuan
Meiyin Zeng
Chunfang Zhang
Chunge Zhang
Huimin Zhang
Jing Zhang
Na Zhang
Tianyuan Zhang
Yi‐Bo Zhang
Yupeng Zhang
Zheng Zhang
Mingda Zhou
Yuanping Zhou
Chengshuai Zhu
Lin Zhu
Yue Zhu
Zhihao Zhu
Hongqin Zou
Anna Zuo
Wenxuan Dong
Tao Wen
Shifu Chen
Guoliang Li
Yunyun Gao
Yong‐Xin Liu
author_facet Defeng Bai
Tong Chen
Jiani Xun
Chuang Ma
Hao Luo
Haifei Yang
Chen Cao
Xiaofeng Cao
Jianzhou Cui
Yuan‐Ping Deng
Zhaochao Deng
Wenxin Dong
Wenxue Dong
Juan Du
Qunkai Fang
Wei Fang
Yue Fang
Fangtian Fu
Min Fu
Yi‐Tian Fu
He Gao
Jingping Ge
Qinglong Gong
Lunda Gu
Peng Guo
Yuhao Guo
Tang Hai
Hao Liu
Jieqiang He
Zi‐Yang He
Huiyu Hou
Can Huang
Shuai Ji
ChangHai Jiang
Gui‐Lai Jiang
Lingjuan Jiang
Ling N. Jin
Yuhe Kan
Da Kang
Jin Kou
Ka‐Lung Lam
Changchao Li
Chong Li
Fuyi Li
Liwei Li
Miao Li
Xin Li
Ye Li
Zheng‐Tao Li
Jing Liang
Yongxin Lin
Changzhen Liu
Danni Liu
Fengqin Liu
Jia Liu
Tianrui Liu
Tingting Liu
Xinyuan Liu
Yaqun Liu
Bangyan Liu
Minghao Liu
Wenbo Lou
Yaning Luan
Yuanyuan Luo
Hujie Lv
Tengfei Ma
Zongjiong Mai
Jiayuan Mo
Dongze Niu
Zhuo Pan
Heyuan Qi
Zhanyao Shi
Chunjiao Song
Fuxiang Sun
Yan Sun
Sihui Tian
Xiulin Wan
Guoliang Wang
Hongyang Wang
Hongyu Wang
Huanhuan Wang
Jing Wang
Jun Wang
Kang Wang
Leli Wang
Shao‐kun Wang
Xinlong Wang
Yao Wang
Zufei Xiao
Huichun Xing
Yifan Xu
Shu‐yan Yan
Li Yang
Song Yang
Yuanming Yang
Xiaofang Yao
Salsabeel Yousuf
Hao Yu
Yu Lei
Zhengrong Yuan
Meiyin Zeng
Chunfang Zhang
Chunge Zhang
Huimin Zhang
Jing Zhang
Na Zhang
Tianyuan Zhang
Yi‐Bo Zhang
Yupeng Zhang
Zheng Zhang
Mingda Zhou
Yuanping Zhou
Chengshuai Zhu
Lin Zhu
Yue Zhu
Zhihao Zhu
Hongqin Zou
Anna Zuo
Wenxuan Dong
Tao Wen
Shifu Chen
Guoliang Li
Yunyun Gao
Yong‐Xin Liu
author_sort Defeng Bai
collection DOAJ
description Abstract Shotgun metagenomics has become a pivotal technology in microbiome research, enabling in‐depth analysis of microbial communities at both the high‐resolution taxonomic and functional levels. This approach provides valuable insights of microbial diversity, interactions, and their roles in health and disease. However, the complexity of data processing and the need for reproducibility pose significant challenges to researchers. To address these challenges, we developed EasyMetagenome, a user‐friendly pipeline that supports multiple analysis methods, including quality control and host removal, read‐based, assembly‐based, and binning, along with advanced genome analysis. The pipeline also features customizable settings, comprehensive data visualizations, and detailed parameter explanations, ensuring its adaptability across a wide range of data scenarios. Looking forward, we aim to refine the pipeline by addressing host contamination issues, optimizing workflows for third‐generation sequencing data, and integrating emerging technologies like deep learning and network analysis, to further enhance microbiome insights and data accuracy. EasyMetageonome is freely available at https://github.com/YongxinLiu/EasyMetagenome.
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spelling doaj-art-9e02ebd1b25b4e5fb372b6e50eee325f2025-08-20T02:45:30ZengWileyiMeta2770-596X2025-02-0141n/an/a10.1002/imt2.70001EasyMetagenome: A user‐friendly and flexible pipeline for shotgun metagenomic analysis in microbiome researchDefeng Bai0Tong Chen1Jiani Xun2Chuang Ma3Hao Luo4Haifei Yang5Chen Cao6Xiaofeng Cao7Jianzhou Cui8Yuan‐Ping Deng9Zhaochao Deng10Wenxin Dong11Wenxue Dong12Juan Du13Qunkai Fang14Wei Fang15Yue Fang16Fangtian Fu17Min Fu18Yi‐Tian Fu19He Gao20Jingping Ge21Qinglong Gong22Lunda Gu23Peng Guo24Yuhao Guo25Tang Hai26Hao Liu27Jieqiang He28Zi‐Yang He29Huiyu Hou30Can Huang31Shuai Ji32ChangHai Jiang33Gui‐Lai Jiang34Lingjuan Jiang35Ling N. Jin36Yuhe Kan37Da Kang38Jin Kou39Ka‐Lung Lam40Changchao Li41Chong Li42Fuyi Li43Liwei Li44Miao Li45Xin Li46Ye Li47Zheng‐Tao Li48Jing Liang49Yongxin Lin50Changzhen Liu51Danni Liu52Fengqin Liu53Jia Liu54Tianrui Liu55Tingting Liu56Xinyuan Liu57Yaqun Liu58Bangyan Liu59Minghao Liu60Wenbo Lou61Yaning Luan62Yuanyuan Luo63Hujie Lv64Tengfei Ma65Zongjiong Mai66Jiayuan Mo67Dongze Niu68Zhuo Pan69Heyuan Qi70Zhanyao Shi71Chunjiao Song72Fuxiang Sun73Yan Sun74Sihui Tian75Xiulin Wan76Guoliang Wang77Hongyang Wang78Hongyu Wang79Huanhuan Wang80Jing Wang81Jun Wang82Kang Wang83Leli Wang84Shao‐kun Wang85Xinlong Wang86Yao Wang87Zufei Xiao88Huichun Xing89Yifan Xu90Shu‐yan Yan91Li Yang92Song Yang93Yuanming Yang94Xiaofang Yao95Salsabeel Yousuf96Hao Yu97Yu Lei98Zhengrong Yuan99Meiyin Zeng100Chunfang Zhang101Chunge Zhang102Huimin Zhang103Jing Zhang104Na Zhang105Tianyuan Zhang106Yi‐Bo Zhang107Yupeng Zhang108Zheng Zhang109Mingda Zhou110Yuanping Zhou111Chengshuai Zhu112Lin Zhu113Yue Zhu114Zhihao Zhu115Hongqin Zou116Anna Zuo117Wenxuan Dong118Tao Wen119Shifu Chen120Guoliang Li121Yunyun Gao122Yong‐Xin Liu123Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao‐di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaKey Laboratory for Bio‐Electromagnetic Environment and Advanced Medical Theranostics, School of Biomedical Engineering and Informatics Nanjing Medical University Nanjing Jiangsu ChinaCenter for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment Tsinghua University Beijing ChinaImmunology Translational Research Programme, Yong Loo Lin School of Medicine National University of Singapore Singapore SingaporeResearch Center for Parasites and Vectors, College of Veterinary Medicine Hunan Agricultural University Changsha Hunan ChinaInstitute of Marine Biology and Pharmacology, Ocean College Zhejiang University Zhoushan Zhejiang ChinaAgro‐Environmental Protection Institute Ministry of Agriculture and Rural Affairs Tianjin ChinaKey Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine Xizang Minzu University Xianyang ChinaKarolinska Institutet, Department of Microbiology, Tumor and Cell Biology Stockholm SwedenCollege of Environment Zhejiang University of Technology Hangzhou ChinaCollege of Environmental and Resource Sciences Zhejiang Agriculture and Forestry University Hangzhou ChinaThe College of Forestry Beijing Forestry University Beijing ChinaDepartment of Bioinformatics, Hangzhou VicrobX Biotech Co., Ltd Hangzhou Zhejiang ChinaAnhui Province Key Laboratory of Integrated Pest Management on Crops, College of Plant Protection Anhui Agricultural University Hefei ChinaXiangya School of Basic Medicine Central South University Changsha Hunan ChinaInstitute of Microbiology,Guangdong Academy of Sciences Guangzhou Guangdong ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education, School of Life Sciences Heilongjiang University Harbin ChinaCollege of Animal Science and Technology Jilin Agricultural University Changchun Jilin ChinaSansure Biotech Incorporation Changsha Hunan ChinaSchool of Food Science and Biology Hebei University of Science and Technology Shijiazhuang Hebei ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education, School of Life Sciences Heilongjiang University Harbin ChinaSchool of Life Sciences Shanxi Datong University Datong ChinaDepartment of Health & Environmental Sciences Xi'an Jiaotong‐Liverpool University Suzhou Jiangsu ChinaCollege of Horticulture Northwest A&F University Yangling Shaanxi ChinaSchool of Agriculture, Food and Ecosystem Sciences, Faculty of Science The University of Melbourne VIC AustraliaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaGraduate School of Frontier Sciences The University of Tokyo Kashiwa‐shi, Chiba JapanInstitute of Biotechnology, Helsinki Institute of Life Science University of Helsinki Helsinki FinlandShanghai Maritime University Shanghai ChinaSuzhou Medical College Soochow University Suzhou Jiangsu ChinaBiomarker Discovery and Validation Facility, Institute of Clinical Medicine, Peking Union Medical College Hospital Beijing ChinaDepartment of Civil and Environmental Engineering The Hong Kong Polytechnic University Hong Kong ChinaCollege of Biology and Oceanography Weifang University Weifang Shandong ChinaCollege of Environmental Science and Engineering Beijing University of Technology Beijing ChinaCollege of Environmental and Municipal Engineering Lanzhou Jiaotong University Lanzhou ChinaSchool of Life Sciences The Chinese University of Hong Kong Shatin, Hong Kong ChinaDepartment of Civil and Environmental Engineering The Hong Kong Polytechnic University Hong Kong ChinaDepartment of Renewable Resources University of Alberta Edmonton Alberta CanadaSchool of Geographical Sciences Northeast Normal University Changchun Jilin ChinaDepartment of Gastroenterology The Second Affiliated Hospital of Guangxi Medical University Nanning Guangxi ChinaSynaura Biotechnology (Shanghai) Co., Ltd. Shanghai ChinaSchool of Public Health University of Michigan Ann Arbor Michigan USAInstitute of Soil Science, Chinese Academy of Sciences Nanjing Jiangsu ChinaSchool of Art and Archaeology of Zhejiang University Zhejiang ChinaCollege of Animal Science and Technology Guangxi University Nanning ChinaFujian Provincial Key Laboratory for Subtropical Resources and Environment Fujian Normal University Fuzhou ChinaCollege of Energy and Environmental Engineering Hebei University of Engineering Handan Hebei ChinaHaploX Biotechnology Shenzhen ChinaCollege of Life Sciences Henan Agricultural University Zhengzhou ChinaCollege of Life Science Nankai University Tianjin ChinaJiangxi Province Key Laboratory of Sustainable Utilization of Traditional Chinese Medicine Resources, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences Jiangxi ChinaBeijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital Capital Medical University Beijing ChinaState Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University Hefei Anhui ChinaSchool of Life Sciences and Food Technology Hanshan Normal University Chaozhou ChinaTsinghua University Beijing ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences Beijing ChinaCollege of Animal Science and Technology Jilin Agricultural University Changchun Jilin ChinaThe College of Forestry Beijing Forestry University Beijing ChinaState Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University Hefei Anhui ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaState Key Laboratory of Herbage Improvement and Grassland Agro‐Ecosystems, Centre for Grassland Microbiome, College of Pastoral Agriculture Science and Technology Lanzhou University Lanzhou Gansu ChinaDepartment of Oncology The Fifth Affiliated Hospital of Sun Yat‐sen University Zhuhai Guangdong ChinaCollege of Animal Science and Technology Guangxi University Nanning ChinaNational‐Local Joint Engineering Research Center of Biomass Refining and High‐Quality Utilization, Institute of Urban and Rural Mining Changzhou University Changzhou Jiangsu ChinaDepartment of Pathology Affiliated Cancer Hospital of Zhengzhou University Zhengzhou ChinaInstitute of Microbiology, Chinese Academy of Sciences Beijing ChinaCollege of Water Sciences Beijing Normal University Beijing ChinaDeyang People's Hospital Deyang Sichuan ChinaNew Direction Biotechnology (Tianjin) Co., Ltd Tianjin ChinaCollege of Energy and Environmental Engineering, Hebei Key Laboratory of Air Pollution Cause and Impact Hebei University of Engineering Handan ChinaInstitute of Botany, Chinese Academy of Sciences Beijing ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaInstitute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences Beijing ChinaNational Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Jiangsu ChinaCollege of Animal Science Anhui Science and Technology University Chuzhou ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences Beijing ChinaChina CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention Beijing ChinaCollege of Animal Science and Technology Yangzhou University Yangzhou Jiangsu ChinaKey Laboratory of Agro‐Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha ChinaInstitute of Ecological Conservation and Restoration, Chinese Academy of Forestry Beijing ChinaBeijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital Capital Medical University Beijing ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaState Key Laboratory for Ecological Security of Regions and Cities, Institute of Urban Environment, Chinese Academy of Sciences Xiamen ChinaCenter of Liver Diseases Division 3, Beijing Ditan Hospital Capital Medical University Beijing ChinaCenter of Liver Diseases Division 3, Beijing Ditan Hospital Capital Medical University Beijing ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Invasive Alien Species Control of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing ChinaSansure Biotech Incorporation Changsha Hunan ChinaCenter of Liver Diseases Division 3, Beijing Ditan Hospital Capital Medical University Beijing ChinaGuangzhou University of Chinese Medicine Guangzhou ChinaKey Laboratory of Agro‐Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaInstitute of Marine Biology and Pharmacology, Ocean College Zhejiang University Zhoushan Zhejiang ChinaKey Laboratory of Livestock Biology Northwest A&F University Yangling Shaanxi ChinaCollege of Biological Sciences and Technology Beijing Forestry University Beijing ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaInstitute of Marine Biology and Pharmacology, Ocean College Zhejiang University Zhoushan Zhejiang ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences Beijing ChinaSchool of Food Science and Technology Shihezi University Shihezi Xinjiang ChinaHaploX Biotechnology Shenzhen ChinaCollege of Biochemical Engineering Beijing Union University Beijing ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Invasive Alien Species Control of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing ChinaCollege of Resources and Environmental Sciences Henan Agricultural University Zhengzhou ChinaTea Research Institute, Chinese Academy of Agricultural Sciences Hangzhou Zhejiang ChinaCollege of Environmental Science and Engineering Tongji University Shanghai ChinaZhanjiang Key Laboratory of Human Microecology and Clinical Translation Research, the Marine Biomedical Research Institute, College of Basic Medicine Guangdong Medical University Zhanjiang Guangdong ChinaSchool of Art and Archaeology of Zhejiang University Zhejiang ChinaState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology Harbin ChinaSchool of Ecology, Environment and Resources Guangdong University of Technology Guangzhou Guangdong ChinaZhanjiang Key Laboratory of Human Microecology and Clinical Translation Research, the Marine Biomedical Research Institute, College of Basic Medicine Guangdong Medical University Zhanjiang Guangdong ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences Beijing ChinaSchool of Traditional Chinese Medicine Southern Medical University Guangzhou Guangdong ChinaDepartment of Animal Sciences Purdue University West Lafayette Indiana USACollege of Resource and Environmental Sciences Nanjing Agricultural University Nanjing Jiangsu ChinaHaploX Biotechnology Shenzhen ChinaJiangxi Provincial Key Laboratory of Conservation Biology, College of Forestry Jiangxi Agricultural University Nanchang Jiangxi ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Guangdong ChinaAbstract Shotgun metagenomics has become a pivotal technology in microbiome research, enabling in‐depth analysis of microbial communities at both the high‐resolution taxonomic and functional levels. This approach provides valuable insights of microbial diversity, interactions, and their roles in health and disease. However, the complexity of data processing and the need for reproducibility pose significant challenges to researchers. To address these challenges, we developed EasyMetagenome, a user‐friendly pipeline that supports multiple analysis methods, including quality control and host removal, read‐based, assembly‐based, and binning, along with advanced genome analysis. The pipeline also features customizable settings, comprehensive data visualizations, and detailed parameter explanations, ensuring its adaptability across a wide range of data scenarios. Looking forward, we aim to refine the pipeline by addressing host contamination issues, optimizing workflows for third‐generation sequencing data, and integrating emerging technologies like deep learning and network analysis, to further enhance microbiome insights and data accuracy. EasyMetageonome is freely available at https://github.com/YongxinLiu/EasyMetagenome.https://doi.org/10.1002/imt2.70001metagenomemicrobiomemicrobiotapipelinevisualization
spellingShingle Defeng Bai
Tong Chen
Jiani Xun
Chuang Ma
Hao Luo
Haifei Yang
Chen Cao
Xiaofeng Cao
Jianzhou Cui
Yuan‐Ping Deng
Zhaochao Deng
Wenxin Dong
Wenxue Dong
Juan Du
Qunkai Fang
Wei Fang
Yue Fang
Fangtian Fu
Min Fu
Yi‐Tian Fu
He Gao
Jingping Ge
Qinglong Gong
Lunda Gu
Peng Guo
Yuhao Guo
Tang Hai
Hao Liu
Jieqiang He
Zi‐Yang He
Huiyu Hou
Can Huang
Shuai Ji
ChangHai Jiang
Gui‐Lai Jiang
Lingjuan Jiang
Ling N. Jin
Yuhe Kan
Da Kang
Jin Kou
Ka‐Lung Lam
Changchao Li
Chong Li
Fuyi Li
Liwei Li
Miao Li
Xin Li
Ye Li
Zheng‐Tao Li
Jing Liang
Yongxin Lin
Changzhen Liu
Danni Liu
Fengqin Liu
Jia Liu
Tianrui Liu
Tingting Liu
Xinyuan Liu
Yaqun Liu
Bangyan Liu
Minghao Liu
Wenbo Lou
Yaning Luan
Yuanyuan Luo
Hujie Lv
Tengfei Ma
Zongjiong Mai
Jiayuan Mo
Dongze Niu
Zhuo Pan
Heyuan Qi
Zhanyao Shi
Chunjiao Song
Fuxiang Sun
Yan Sun
Sihui Tian
Xiulin Wan
Guoliang Wang
Hongyang Wang
Hongyu Wang
Huanhuan Wang
Jing Wang
Jun Wang
Kang Wang
Leli Wang
Shao‐kun Wang
Xinlong Wang
Yao Wang
Zufei Xiao
Huichun Xing
Yifan Xu
Shu‐yan Yan
Li Yang
Song Yang
Yuanming Yang
Xiaofang Yao
Salsabeel Yousuf
Hao Yu
Yu Lei
Zhengrong Yuan
Meiyin Zeng
Chunfang Zhang
Chunge Zhang
Huimin Zhang
Jing Zhang
Na Zhang
Tianyuan Zhang
Yi‐Bo Zhang
Yupeng Zhang
Zheng Zhang
Mingda Zhou
Yuanping Zhou
Chengshuai Zhu
Lin Zhu
Yue Zhu
Zhihao Zhu
Hongqin Zou
Anna Zuo
Wenxuan Dong
Tao Wen
Shifu Chen
Guoliang Li
Yunyun Gao
Yong‐Xin Liu
EasyMetagenome: A user‐friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
iMeta
metagenome
microbiome
microbiota
pipeline
visualization
title EasyMetagenome: A user‐friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
title_full EasyMetagenome: A user‐friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
title_fullStr EasyMetagenome: A user‐friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
title_full_unstemmed EasyMetagenome: A user‐friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
title_short EasyMetagenome: A user‐friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
title_sort easymetagenome a user friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
topic metagenome
microbiome
microbiota
pipeline
visualization
url https://doi.org/10.1002/imt2.70001
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