The Effects of <i>VEGF-A</i> and <i>GSTM1</i>/<i>GSTT1</i> Variants in the Susceptibility to the Chronic Rhinosinusitis with Nasal Polyposis: A Pilot Genetic Study
Nasal polyps (NPs) are usually part of chronic rhinosinusitis with nasal polyposis (CRSwNP). However, the exact etiology of CRSwNP is still unknown. In addition, the suggested etiological causes are infection, allergy, and immunological disorders, among others, such as genetic predisposition. Moreov...
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2024-10-01
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| author | Leandro Azevedo Camargo Angela Adamski da Silva Reis Stela Oliveira Rodrigues Rodrigo da Silva Santos Melissa Ameloti Gomes Avelino |
| author_facet | Leandro Azevedo Camargo Angela Adamski da Silva Reis Stela Oliveira Rodrigues Rodrigo da Silva Santos Melissa Ameloti Gomes Avelino |
| author_sort | Leandro Azevedo Camargo |
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| description | Nasal polyps (NPs) are usually part of chronic rhinosinusitis with nasal polyposis (CRSwNP). However, the exact etiology of CRSwNP is still unknown. In addition, the suggested etiological causes are infection, allergy, and immunological disorders, among others, such as genetic predisposition. Moreover, it is also suggested that oxygen-free radicals play a vital role in the pathogenesis of nasal polyposis, and inflammatory cells produce free radicals during phagocytosis, which is the primary source of ROS, controlled by the glutathione S-transferase (GST) system. Although, vascular endothelial growth factor (VEGF) plays an important role in angiogenesis, it is closely interwoven with the mobilization of inflammatory cells. This pilot study evaluated the association between genetic variant <i>VEGF-A</i> (rs28357093) and <i>GSTM1</i>/<i>GSTT1</i> deletion polymorphism in susceptibility to CRSwNP. A case–control study was conducted with 61 individuals diagnosed with CRSwNP and 100 healthy subjects. <i>VEGF-A</i> (rs28357093) and <i>GSTM1</i>/<i>GSTT1</i> deletion polymorphisms were genotyped by RFLP-PCR and SYBR Green real-time PCR, respectively. Individuals with allergic rhinitis carriers with AC genotype (rs28357093) presented a 4-fold increased risk to CRSwNP (OR = 4.20, 95% CI = 1.31 to 13.50; <i>p</i> = 0.015). This evidence shows that the increased vascular permeability probably causes an inflamed nasal area leading to extensive edema and polyp growth. On the other hand, no association was verified for each genetic variant by inheritance models. Interestingly, the <i>GSTT1</i> present genotype showed a protective effect on CRSwNP. In conclusion, additional studies that have larger groups in different geographic localizations may be useful to verify and assess the association between genetic variants and CRSwNP. |
| format | Article |
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| institution | OA Journals |
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| language | English |
| publishDate | 2024-10-01 |
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| series | Biomedicines |
| spelling | doaj-art-9dee7a9c9552406988b3828babfe0bf22025-08-20T02:11:04ZengMDPI AGBiomedicines2227-90592024-10-011210238310.3390/biomedicines12102383The Effects of <i>VEGF-A</i> and <i>GSTM1</i>/<i>GSTT1</i> Variants in the Susceptibility to the Chronic Rhinosinusitis with Nasal Polyposis: A Pilot Genetic StudyLeandro Azevedo Camargo0Angela Adamski da Silva Reis1Stela Oliveira Rodrigues2Rodrigo da Silva Santos3Melissa Ameloti Gomes Avelino4Departamento de Cirurgia, Faculdade de Medicina, Universidade Federal de Goiás, Goiânia 74.690-900, GO, BrazilDepartamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia 74.690-900, GO, BrazilDepartamento de Cirurgia, Faculdade de Medicina, Universidade Federal de Goiás, Goiânia 74.690-900, GO, BrazilDepartamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia 74.690-900, GO, BrazilDepartamento de Cirurgia, Faculdade de Medicina, Universidade Federal de Goiás, Goiânia 74.690-900, GO, BrazilNasal polyps (NPs) are usually part of chronic rhinosinusitis with nasal polyposis (CRSwNP). However, the exact etiology of CRSwNP is still unknown. In addition, the suggested etiological causes are infection, allergy, and immunological disorders, among others, such as genetic predisposition. Moreover, it is also suggested that oxygen-free radicals play a vital role in the pathogenesis of nasal polyposis, and inflammatory cells produce free radicals during phagocytosis, which is the primary source of ROS, controlled by the glutathione S-transferase (GST) system. Although, vascular endothelial growth factor (VEGF) plays an important role in angiogenesis, it is closely interwoven with the mobilization of inflammatory cells. This pilot study evaluated the association between genetic variant <i>VEGF-A</i> (rs28357093) and <i>GSTM1</i>/<i>GSTT1</i> deletion polymorphism in susceptibility to CRSwNP. A case–control study was conducted with 61 individuals diagnosed with CRSwNP and 100 healthy subjects. <i>VEGF-A</i> (rs28357093) and <i>GSTM1</i>/<i>GSTT1</i> deletion polymorphisms were genotyped by RFLP-PCR and SYBR Green real-time PCR, respectively. Individuals with allergic rhinitis carriers with AC genotype (rs28357093) presented a 4-fold increased risk to CRSwNP (OR = 4.20, 95% CI = 1.31 to 13.50; <i>p</i> = 0.015). This evidence shows that the increased vascular permeability probably causes an inflamed nasal area leading to extensive edema and polyp growth. On the other hand, no association was verified for each genetic variant by inheritance models. Interestingly, the <i>GSTT1</i> present genotype showed a protective effect on CRSwNP. In conclusion, additional studies that have larger groups in different geographic localizations may be useful to verify and assess the association between genetic variants and CRSwNP.https://www.mdpi.com/2227-9059/12/10/2383vascular permeabilityinflammationoxidative stressBrazilian population |
| spellingShingle | Leandro Azevedo Camargo Angela Adamski da Silva Reis Stela Oliveira Rodrigues Rodrigo da Silva Santos Melissa Ameloti Gomes Avelino The Effects of <i>VEGF-A</i> and <i>GSTM1</i>/<i>GSTT1</i> Variants in the Susceptibility to the Chronic Rhinosinusitis with Nasal Polyposis: A Pilot Genetic Study Biomedicines vascular permeability inflammation oxidative stress Brazilian population |
| title | The Effects of <i>VEGF-A</i> and <i>GSTM1</i>/<i>GSTT1</i> Variants in the Susceptibility to the Chronic Rhinosinusitis with Nasal Polyposis: A Pilot Genetic Study |
| title_full | The Effects of <i>VEGF-A</i> and <i>GSTM1</i>/<i>GSTT1</i> Variants in the Susceptibility to the Chronic Rhinosinusitis with Nasal Polyposis: A Pilot Genetic Study |
| title_fullStr | The Effects of <i>VEGF-A</i> and <i>GSTM1</i>/<i>GSTT1</i> Variants in the Susceptibility to the Chronic Rhinosinusitis with Nasal Polyposis: A Pilot Genetic Study |
| title_full_unstemmed | The Effects of <i>VEGF-A</i> and <i>GSTM1</i>/<i>GSTT1</i> Variants in the Susceptibility to the Chronic Rhinosinusitis with Nasal Polyposis: A Pilot Genetic Study |
| title_short | The Effects of <i>VEGF-A</i> and <i>GSTM1</i>/<i>GSTT1</i> Variants in the Susceptibility to the Chronic Rhinosinusitis with Nasal Polyposis: A Pilot Genetic Study |
| title_sort | effects of i vegf a i and i gstm1 i i gstt1 i variants in the susceptibility to the chronic rhinosinusitis with nasal polyposis a pilot genetic study |
| topic | vascular permeability inflammation oxidative stress Brazilian population |
| url | https://www.mdpi.com/2227-9059/12/10/2383 |
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