The role of sodium bicarbonate in improving cold storage preservation of indigenous black rabbit spermatozoa
The population of indigenous black rabbits in Viet Nam has been steadily declining, primarily due to the increasing preference for commercial meat rabbit varieties. Consequently, the conservation and systematic breeding of indigenous black rabbits in the Mekong Delta are critical for preserving gen...
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| Language: | English |
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Can Tho University Publisher
2025-03-01
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| Series: | CTU Journal of Innovation and Sustainable Development |
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| Online Access: | https://ctujs.ctu.edu.vn/index.php/ctujs/article/view/940 |
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| author | Thi Thanh Khuong Tran Nhut Anh Lam Trong Ngu Nguyen Lam Khanh Duy Nguyen |
| author_facet | Thi Thanh Khuong Tran Nhut Anh Lam Trong Ngu Nguyen Lam Khanh Duy Nguyen |
| author_sort | Thi Thanh Khuong Tran |
| collection | DOAJ |
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The population of indigenous black rabbits in Viet Nam has been steadily declining, primarily due to the increasing preference for commercial meat rabbit varieties. Consequently, the conservation and systematic breeding of indigenous black rabbits in the Mekong Delta are critical for preserving genetic diversity and sustaining local biodiversity. Sperm conservation is essential for the artificial reproduction of this species. This study aimed to find the optimal concentration of sodium bicarbonate (NaHCO3) buffer added to Tris Citrate Glucose (TCG) storage medium. Samples after collection were diluted with a storage medium containing sodium bicarbonate at concentrations of 0 mM, 15 mM, 25 mM and 35 mM, then refrigerated at 15ᵒC. The results showed that rabbit sperm achieved the best quality when supplemented with 25 mM NaHCO3 after 72 hours of storage. Specifically, the sperms had 59.25% overall motility, 22.34% progressive motility, 62.19% viability and 46.77% membrane integrity, the differences were statistically significant when compared with the remaining treatments (p<0.05). The study results indicate that supplementing the TCG preservation medium with 25 mM NaHCO3 significantly improves the quality of sperm during cold storage. This study has contributed to the development of the sperm conservation medium for indigenous black rabbits in the Mekong Delta.
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| format | Article |
| id | doaj-art-9de831f924a54986bbffae6c963657da |
| institution | OA Journals |
| issn | 2588-1418 2815-6412 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Can Tho University Publisher |
| record_format | Article |
| series | CTU Journal of Innovation and Sustainable Development |
| spelling | doaj-art-9de831f924a54986bbffae6c963657da2025-08-20T02:16:49ZengCan Tho University PublisherCTU Journal of Innovation and Sustainable Development2588-14182815-64122025-03-0117110.22144/ctujoisd.2025.021The role of sodium bicarbonate in improving cold storage preservation of indigenous black rabbit spermatozoaThi Thanh Khuong Tran0Nhut Anh Lam1Trong Ngu Nguyen2Lam Khanh Duy Nguyen3BM.Cong nghe Sinh hoc Phan tu, Viện NC&PT Công nghệ Sinh họcStem cell Laboratory, Institute of Food and Biotechnology, Can Tho University, Can Tho city,90000, Vietnam Faculty of Animal Sciences, College of Agriculture, Can Tho University, Can Tho City, VietnamStem cell Laboratory, Institute of Food and Biotechnology, Can Tho University, Can Tho city,90000, Vietnam The population of indigenous black rabbits in Viet Nam has been steadily declining, primarily due to the increasing preference for commercial meat rabbit varieties. Consequently, the conservation and systematic breeding of indigenous black rabbits in the Mekong Delta are critical for preserving genetic diversity and sustaining local biodiversity. Sperm conservation is essential for the artificial reproduction of this species. This study aimed to find the optimal concentration of sodium bicarbonate (NaHCO3) buffer added to Tris Citrate Glucose (TCG) storage medium. Samples after collection were diluted with a storage medium containing sodium bicarbonate at concentrations of 0 mM, 15 mM, 25 mM and 35 mM, then refrigerated at 15ᵒC. The results showed that rabbit sperm achieved the best quality when supplemented with 25 mM NaHCO3 after 72 hours of storage. Specifically, the sperms had 59.25% overall motility, 22.34% progressive motility, 62.19% viability and 46.77% membrane integrity, the differences were statistically significant when compared with the remaining treatments (p<0.05). The study results indicate that supplementing the TCG preservation medium with 25 mM NaHCO3 significantly improves the quality of sperm during cold storage. This study has contributed to the development of the sperm conservation medium for indigenous black rabbits in the Mekong Delta. https://ctujs.ctu.edu.vn/index.php/ctujs/article/view/940Indigenous black rabbit, motility, sodium bicarbonate buffer, sperm |
| spellingShingle | Thi Thanh Khuong Tran Nhut Anh Lam Trong Ngu Nguyen Lam Khanh Duy Nguyen The role of sodium bicarbonate in improving cold storage preservation of indigenous black rabbit spermatozoa CTU Journal of Innovation and Sustainable Development Indigenous black rabbit, motility, sodium bicarbonate buffer, sperm |
| title | The role of sodium bicarbonate in improving cold storage preservation of indigenous black rabbit spermatozoa |
| title_full | The role of sodium bicarbonate in improving cold storage preservation of indigenous black rabbit spermatozoa |
| title_fullStr | The role of sodium bicarbonate in improving cold storage preservation of indigenous black rabbit spermatozoa |
| title_full_unstemmed | The role of sodium bicarbonate in improving cold storage preservation of indigenous black rabbit spermatozoa |
| title_short | The role of sodium bicarbonate in improving cold storage preservation of indigenous black rabbit spermatozoa |
| title_sort | role of sodium bicarbonate in improving cold storage preservation of indigenous black rabbit spermatozoa |
| topic | Indigenous black rabbit, motility, sodium bicarbonate buffer, sperm |
| url | https://ctujs.ctu.edu.vn/index.php/ctujs/article/view/940 |
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