Influence of olfactory learning on host selection behavior in Bactrocera dorsalis (Diptera: Tephritidae)

Insects can search for host more efficiently by olfactory learning. Bactrocera dorsalis has been proved that it has strong olfactory learning capability. To explore the influence of olfactory learning on its host selection behavior, we trained B. dorsalis to associate an odor, sweet orange essential...

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Main Authors: YU Jinxin, FANG Chao, ZHOU Jiayi, YAO Yuanyuan, ZENG Xinnian, LIU Jiali
Format: Article
Language:English
Published: Zhejiang University Press 2019-04-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.05.071
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author YU Jinxin
FANG Chao
ZHOU Jiayi
YAO Yuanyuan
ZENG Xinnian
LIU Jiali
author_facet YU Jinxin
FANG Chao
ZHOU Jiayi
YAO Yuanyuan
ZENG Xinnian
LIU Jiali
author_sort YU Jinxin
collection DOAJ
description Insects can search for host more efficiently by olfactory learning. Bactrocera dorsalis has been proved that it has strong olfactory learning capability. To explore the influence of olfactory learning on its host selection behavior, we trained B. dorsalis to associate an odor, sweet orange essential oil, with a food, 10% sucrose solution, under laboratory conditions using a classical olfactory conditioning procedure, and the proboscis extension reflex was used as the criterion for conditioning. Its preference for orange odor and orientation behavior elicited by orange odor before and after training also were tested. The selection behavior and trend behavior of B. dorsalis after learning were determined. The results showed that B. dorsalis can associate the orange odor with the sucrose solution, and the proportion of proboscis extension reflex of B. dorsalis increased from 8.1% to 75.7% after five conditioning trials, and the memory formed with conditioning could last at least 6 h. In the Y-tube choice test, B. dorsalis chose the arm containing trained orange odor more often after five conditioning trials compared with before training (increased from 35.3% to 63.7%). In the wind tunnel test, the percentage of B. dorsalis located orange odor after conditioning increased from 8.3% to 52.5% compared with before conditioning. The results indicated that B. dorsalis has a strong capability to learn orange odor, and they tended to choose the trained orange odor after learning, which might be related with host searching behavior. Our studies also can provide an important reference on behavior regulation of B. dorsalis.
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spelling doaj-art-9de45985e3e541918ea76d8a42103ee02025-08-20T03:16:03ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552019-04-014518118810.3785/j.issn.1008-9209.2018.05.07110089209Influence of olfactory learning on host selection behavior in Bactrocera dorsalis (Diptera: Tephritidae)YU JinxinFANG ChaoZHOU JiayiYAO YuanyuanZENG XinnianLIU JialiInsects can search for host more efficiently by olfactory learning. Bactrocera dorsalis has been proved that it has strong olfactory learning capability. To explore the influence of olfactory learning on its host selection behavior, we trained B. dorsalis to associate an odor, sweet orange essential oil, with a food, 10% sucrose solution, under laboratory conditions using a classical olfactory conditioning procedure, and the proboscis extension reflex was used as the criterion for conditioning. Its preference for orange odor and orientation behavior elicited by orange odor before and after training also were tested. The selection behavior and trend behavior of B. dorsalis after learning were determined. The results showed that B. dorsalis can associate the orange odor with the sucrose solution, and the proportion of proboscis extension reflex of B. dorsalis increased from 8.1% to 75.7% after five conditioning trials, and the memory formed with conditioning could last at least 6 h. In the Y-tube choice test, B. dorsalis chose the arm containing trained orange odor more often after five conditioning trials compared with before training (increased from 35.3% to 63.7%). In the wind tunnel test, the percentage of B. dorsalis located orange odor after conditioning increased from 8.3% to 52.5% compared with before conditioning. The results indicated that B. dorsalis has a strong capability to learn orange odor, and they tended to choose the trained orange odor after learning, which might be related with host searching behavior. Our studies also can provide an important reference on behavior regulation of B. dorsalis.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.05.071<italic>Bactrocera dorsalis</italic>host selection behaviorclassical conditioningappetitive conditioningproboscis extension reflex
spellingShingle YU Jinxin
FANG Chao
ZHOU Jiayi
YAO Yuanyuan
ZENG Xinnian
LIU Jiali
Influence of olfactory learning on host selection behavior in Bactrocera dorsalis (Diptera: Tephritidae)
浙江大学学报. 农业与生命科学版
<italic>Bactrocera dorsalis</italic>
host selection behavior
classical conditioning
appetitive conditioning
proboscis extension reflex
title Influence of olfactory learning on host selection behavior in Bactrocera dorsalis (Diptera: Tephritidae)
title_full Influence of olfactory learning on host selection behavior in Bactrocera dorsalis (Diptera: Tephritidae)
title_fullStr Influence of olfactory learning on host selection behavior in Bactrocera dorsalis (Diptera: Tephritidae)
title_full_unstemmed Influence of olfactory learning on host selection behavior in Bactrocera dorsalis (Diptera: Tephritidae)
title_short Influence of olfactory learning on host selection behavior in Bactrocera dorsalis (Diptera: Tephritidae)
title_sort influence of olfactory learning on host selection behavior in bactrocera dorsalis diptera tephritidae
topic <italic>Bactrocera dorsalis</italic>
host selection behavior
classical conditioning
appetitive conditioning
proboscis extension reflex
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.05.071
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AT fangchao influenceofolfactorylearningonhostselectionbehaviorinbactroceradorsalisdipteratephritidae
AT zhoujiayi influenceofolfactorylearningonhostselectionbehaviorinbactroceradorsalisdipteratephritidae
AT yaoyuanyuan influenceofolfactorylearningonhostselectionbehaviorinbactroceradorsalisdipteratephritidae
AT zengxinnian influenceofolfactorylearningonhostselectionbehaviorinbactroceradorsalisdipteratephritidae
AT liujiali influenceofolfactorylearningonhostselectionbehaviorinbactroceradorsalisdipteratephritidae