Elevated Expression of the Long Noncoding RNA IFNG-AS1 in the Peripheral Blood from Patients with Rheumatoid Arthritis
Long noncoding RNAs (lncRNAs) have been increasingly recognized as key immune molecules that participate in the pathogenesis of autoimmune diseases. Previous studies have demonstrated that the lncRNA Ifng-AS1, a key scaffold that contributes to the transcription of IFN-γ, depends on T-bet for active...
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/6401978 |
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| author | Huiyong Peng Shuting Ren Yingzhao Liu Huimin Zhou Xinyi Tang Jun Yang Jie Tian Ping Xu Huaxi Xu Shengjun Wang |
| author_facet | Huiyong Peng Shuting Ren Yingzhao Liu Huimin Zhou Xinyi Tang Jun Yang Jie Tian Ping Xu Huaxi Xu Shengjun Wang |
| author_sort | Huiyong Peng |
| collection | DOAJ |
| description | Long noncoding RNAs (lncRNAs) have been increasingly recognized as key immune molecules that participate in the pathogenesis of autoimmune diseases. Previous studies have demonstrated that the lncRNA Ifng-AS1, a key scaffold that contributes to the transcription of IFN-γ, depends on T-bet for active transcription in Th1 cells. However, the effect of its human ortholog, IFNG-AS1, on the pathogenesis of rheumatoid arthritis (RA) remains unclear. In this study, we found that the transcript level of lncRNA IFNG-AS1 was increased in the peripheral blood of RA patients. IFNG, as a target gene of IFNG-AS1, was overexpressed and positively correlated with the transcript level of IFNG-AS1 in the RA patients. Our data also showed that the transcript level of T-bet was upregulated and positively correlated with IFNG-AS1 expression. T-bet regulated the transcription of IFNG-AS1 in human CD4+ T cells in vitro. Furthermore, strong positive correlations were observed between the increased transcript level of IFNG-AS1 and the serum level of rheumatoid factor, the erythrocyte sedimentation rate, and the C-reactive protein in RA patients, and patients positive for anticyclic citrullinated peptide antibodies had increased levels of IFNG-AS1. Finally, receiver operating characteristic (ROC) curve analysis suggested that IFNG-AS1 might be a potential biomarker of RA. Taken together, our findings indicated that IFNG-AS1, guided by T-bet, is augmented in the peripheral blood of RA patients and may play a critical role in the pathogenesis of RA by regulating the expression of IFNG. |
| format | Article |
| id | doaj-art-9ddeebcea27c410f822c2dcb8aab6c1f |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
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| series | Journal of Immunology Research |
| spelling | doaj-art-9ddeebcea27c410f822c2dcb8aab6c1f2025-08-20T02:20:27ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/64019786401978Elevated Expression of the Long Noncoding RNA IFNG-AS1 in the Peripheral Blood from Patients with Rheumatoid ArthritisHuiyong Peng0Shuting Ren1Yingzhao Liu2Huimin Zhou3Xinyi Tang4Jun Yang5Jie Tian6Ping Xu7Huaxi Xu8Shengjun Wang9Department of Laboratory Medicine, The Affiliated People’s Hospital, Jiangsu University, Zhenjiang 212002, ChinaDepartment of Laboratory Medicine, The PLA 904 Hospital, Wuxi 214044, ChinaDepartment of Endocrinology, The Affiliated People’s Hospital, Jiangsu University, Zhenjiang 212002, ChinaInstitute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang 212013, ChinaDepartment of Laboratory Medicine, The Affiliated People’s Hospital, Jiangsu University, Zhenjiang 212002, ChinaDepartment of Laboratory Medicine, The Affiliated People’s Hospital, Jiangsu University, Zhenjiang 212002, ChinaInstitute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang 212013, ChinaDepartment of Laboratory Medicine, The Fifth People’s Hospital of Suzhou, Suzhou, ChinaInstitute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang 212013, ChinaDepartment of Laboratory Medicine, The Affiliated People’s Hospital, Jiangsu University, Zhenjiang 212002, ChinaLong noncoding RNAs (lncRNAs) have been increasingly recognized as key immune molecules that participate in the pathogenesis of autoimmune diseases. Previous studies have demonstrated that the lncRNA Ifng-AS1, a key scaffold that contributes to the transcription of IFN-γ, depends on T-bet for active transcription in Th1 cells. However, the effect of its human ortholog, IFNG-AS1, on the pathogenesis of rheumatoid arthritis (RA) remains unclear. In this study, we found that the transcript level of lncRNA IFNG-AS1 was increased in the peripheral blood of RA patients. IFNG, as a target gene of IFNG-AS1, was overexpressed and positively correlated with the transcript level of IFNG-AS1 in the RA patients. Our data also showed that the transcript level of T-bet was upregulated and positively correlated with IFNG-AS1 expression. T-bet regulated the transcription of IFNG-AS1 in human CD4+ T cells in vitro. Furthermore, strong positive correlations were observed between the increased transcript level of IFNG-AS1 and the serum level of rheumatoid factor, the erythrocyte sedimentation rate, and the C-reactive protein in RA patients, and patients positive for anticyclic citrullinated peptide antibodies had increased levels of IFNG-AS1. Finally, receiver operating characteristic (ROC) curve analysis suggested that IFNG-AS1 might be a potential biomarker of RA. Taken together, our findings indicated that IFNG-AS1, guided by T-bet, is augmented in the peripheral blood of RA patients and may play a critical role in the pathogenesis of RA by regulating the expression of IFNG.http://dx.doi.org/10.1155/2020/6401978 |
| spellingShingle | Huiyong Peng Shuting Ren Yingzhao Liu Huimin Zhou Xinyi Tang Jun Yang Jie Tian Ping Xu Huaxi Xu Shengjun Wang Elevated Expression of the Long Noncoding RNA IFNG-AS1 in the Peripheral Blood from Patients with Rheumatoid Arthritis Journal of Immunology Research |
| title | Elevated Expression of the Long Noncoding RNA IFNG-AS1 in the Peripheral Blood from Patients with Rheumatoid Arthritis |
| title_full | Elevated Expression of the Long Noncoding RNA IFNG-AS1 in the Peripheral Blood from Patients with Rheumatoid Arthritis |
| title_fullStr | Elevated Expression of the Long Noncoding RNA IFNG-AS1 in the Peripheral Blood from Patients with Rheumatoid Arthritis |
| title_full_unstemmed | Elevated Expression of the Long Noncoding RNA IFNG-AS1 in the Peripheral Blood from Patients with Rheumatoid Arthritis |
| title_short | Elevated Expression of the Long Noncoding RNA IFNG-AS1 in the Peripheral Blood from Patients with Rheumatoid Arthritis |
| title_sort | elevated expression of the long noncoding rna ifng as1 in the peripheral blood from patients with rheumatoid arthritis |
| url | http://dx.doi.org/10.1155/2020/6401978 |
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