Protective Role of Ramipril and Candesartan against Myocardial Ischemic Reperfusion Injury: A Biochemical and Transmission Electron Microscopical Study
The present study was designed to investigate the role of combined administration of Ramipril and Candesartan against in vitro myocardial ischemic reperfusion injury in rat. Male Wistar albino rats were divided into five groups (n=6) and treated with saline (10 mL/kg), Ramipril (2 mg/kg), Candesarta...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Advances in Pharmacological Sciences |
| Online Access: | http://dx.doi.org/10.1155/2016/4608979 |
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| author | Mohamed Saleem Thattakudian Sheik Uduman Rajitha Bodd Reddy Priyanka Punuru Gopinath Chakka Gauthaman Karunakaran |
| author_facet | Mohamed Saleem Thattakudian Sheik Uduman Rajitha Bodd Reddy Priyanka Punuru Gopinath Chakka Gauthaman Karunakaran |
| author_sort | Mohamed Saleem Thattakudian Sheik Uduman |
| collection | DOAJ |
| description | The present study was designed to investigate the role of combined administration of Ramipril and Candesartan against in vitro myocardial ischemic reperfusion injury in rat. Male Wistar albino rats were divided into five groups (n=6) and treated with saline (10 mL/kg), Ramipril (2 mg/kg), Candesartan (1 mg/kg), and the combination of both drugs, respectively 24 h before induction of global ischemia (5 min of stabilization, 9 min of global ischemia, and 12 min of reflow). Combination of Ramipril and Candesartan when compared to the monotherapy significantly increased the levels of superoxide dismutase, reduced glutathione, catalase, and nitric oxide and decreased the levels of thiobarbituric acid reactive substances. In addition, the superior protective role of combination of Ramipril and Candesartan on ischemia induced myocardial damage was further confirmed by well preserved myocardial tissue architecture in light microscopy and transmission electron microscopy analysis studies. The combination was proved to be effective in salvaging the myocardial tissue against ischemic reperfusion injury when compared to the monotherapy of individual drugs and further investigations on protective mechanism of drugs by increasing the nitric oxide level at molecular levels are needed. |
| format | Article |
| id | doaj-art-9dddbf5c2879491899fb2c6e5b5bc9a5 |
| institution | OA Journals |
| issn | 1687-6334 1687-6342 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological Sciences |
| spelling | doaj-art-9dddbf5c2879491899fb2c6e5b5bc9a52025-08-20T02:20:27ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422016-01-01201610.1155/2016/46089794608979Protective Role of Ramipril and Candesartan against Myocardial Ischemic Reperfusion Injury: A Biochemical and Transmission Electron Microscopical StudyMohamed Saleem Thattakudian Sheik Uduman0Rajitha Bodd Reddy1Priyanka Punuru2Gopinath Chakka3Gauthaman Karunakaran4Department of Pharmacology, Annamacharya College of Pharmacy, Rajampet 516126, IndiaDepartment of Pharmacology, Annamacharya College of Pharmacy, Rajampet 516126, IndiaDepartment of Pharmacology, Annamacharya College of Pharmacy, Rajampet 516126, IndiaDepartment of Pharmacology, Annamacharya College of Pharmacy, Rajampet 516126, IndiaDepartment of Pharmacology, College of Pharmacy, King Khalid University, Abha 61441, Saudi ArabiaThe present study was designed to investigate the role of combined administration of Ramipril and Candesartan against in vitro myocardial ischemic reperfusion injury in rat. Male Wistar albino rats were divided into five groups (n=6) and treated with saline (10 mL/kg), Ramipril (2 mg/kg), Candesartan (1 mg/kg), and the combination of both drugs, respectively 24 h before induction of global ischemia (5 min of stabilization, 9 min of global ischemia, and 12 min of reflow). Combination of Ramipril and Candesartan when compared to the monotherapy significantly increased the levels of superoxide dismutase, reduced glutathione, catalase, and nitric oxide and decreased the levels of thiobarbituric acid reactive substances. In addition, the superior protective role of combination of Ramipril and Candesartan on ischemia induced myocardial damage was further confirmed by well preserved myocardial tissue architecture in light microscopy and transmission electron microscopy analysis studies. The combination was proved to be effective in salvaging the myocardial tissue against ischemic reperfusion injury when compared to the monotherapy of individual drugs and further investigations on protective mechanism of drugs by increasing the nitric oxide level at molecular levels are needed.http://dx.doi.org/10.1155/2016/4608979 |
| spellingShingle | Mohamed Saleem Thattakudian Sheik Uduman Rajitha Bodd Reddy Priyanka Punuru Gopinath Chakka Gauthaman Karunakaran Protective Role of Ramipril and Candesartan against Myocardial Ischemic Reperfusion Injury: A Biochemical and Transmission Electron Microscopical Study Advances in Pharmacological Sciences |
| title | Protective Role of Ramipril and Candesartan against Myocardial Ischemic Reperfusion Injury: A Biochemical and Transmission Electron Microscopical Study |
| title_full | Protective Role of Ramipril and Candesartan against Myocardial Ischemic Reperfusion Injury: A Biochemical and Transmission Electron Microscopical Study |
| title_fullStr | Protective Role of Ramipril and Candesartan against Myocardial Ischemic Reperfusion Injury: A Biochemical and Transmission Electron Microscopical Study |
| title_full_unstemmed | Protective Role of Ramipril and Candesartan against Myocardial Ischemic Reperfusion Injury: A Biochemical and Transmission Electron Microscopical Study |
| title_short | Protective Role of Ramipril and Candesartan against Myocardial Ischemic Reperfusion Injury: A Biochemical and Transmission Electron Microscopical Study |
| title_sort | protective role of ramipril and candesartan against myocardial ischemic reperfusion injury a biochemical and transmission electron microscopical study |
| url | http://dx.doi.org/10.1155/2016/4608979 |
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