Characterizing Methicillin-Resistant <i>Staphylococcus</i> spp. and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> in Cattle
In the field of cattle medicine in Austria, to date, few studies have investigated the presence of methicillin-resistant <i>Staphylococcus aureus</i> and extended-spectrum β-lactamase-producing <i>Escherichia coli</i> in Austria. For this reason, milk and nasal samples were e...
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2024-11-01
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| author | Lisa Abdank Igor Loncaric Sascha D. Braun Elke Müller Stefan Monecke Ralf Ehricht Reinhild Krametter-Frötscher |
| author_facet | Lisa Abdank Igor Loncaric Sascha D. Braun Elke Müller Stefan Monecke Ralf Ehricht Reinhild Krametter-Frötscher |
| author_sort | Lisa Abdank |
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| description | In the field of cattle medicine in Austria, to date, few studies have investigated the presence of methicillin-resistant <i>Staphylococcus aureus</i> and extended-spectrum β-lactamase-producing <i>Escherichia coli</i> in Austria. For this reason, milk and nasal samples were examined for the presence of methicillin-resistant <i>Staphylococcus aureus</i> as well as fecal samples for extended-spectrum cephalosporin-resistant <i>Escherichia coli</i>. The nasal and fecal swabs were collected during the veterinary treatment of calf pneumonia and calf diarrhea. For the milk samples, the first milk jets were milked into a pre-milking cup and then the teats were cleaned and disinfected before the samples were taken. The cows were selected during the veterinary visits to the farms when treatment was necessary due to mastitis. Depending on the severity of the mastitis (acute mastitis or subclinical mastitis), antibiotics and non-steroidal anti-inflammatory drugs were given immediately (acute disease) or after completion of the antibiogram (subclinical disease). Isolates were characterized by a polyphasic approach including susceptibility pheno- and genotyping and microarray-based assays. No methicillin-resistant <i>Staphylococcus aureus</i> was found in the milk samples, but one nasal swab was positive for methicillin-resistant <i>Staphylococcus aureus</i>. Twenty-two <i>Escherichia coli</i> isolates were detected among the fecal samples. All the <i>Escherichia coli</i> isolates were resistant to ceftazidime. In all the <i>Escherichia coli</i> isolates, genes from the <i>bla</i><sub>CTX</sub> family were detected with other <i>bla</i> genes or alone; the most frequently observed β-lactamase gene was <i>bla</i><sub>CTX-M-1/15</sub> (n = 20). In total, 63.6% (n = 14) of the isolates exhibited a multidrug-resistant phenotype and one <i>E. coli</i> isolate (4.5%) harbored the AmpC gene. Precisely because the presence of data regarding extended-spectrum cephalosporin-resistant <i>Escherichia coli</i> and methicillin-resistant <i>Staphylococcus aureus</i> in calves and cows in Austria is rare, this study further expands our understanding of antimicrobial resistance in Austrian cattle, which is highly relevant for successful antibiotic therapy in sick cattle. |
| format | Article |
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| publishDate | 2024-11-01 |
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| spelling | doaj-art-9ddcdeb14a3546b59c0d766358cb48c92025-08-20T02:50:14ZengMDPI AGAnimals2076-26152024-11-011423338310.3390/ani14233383Characterizing Methicillin-Resistant <i>Staphylococcus</i> spp. and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> in CattleLisa Abdank0Igor Loncaric1Sascha D. Braun2Elke Müller3Stefan Monecke4Ralf Ehricht5Reinhild Krametter-Frötscher6Clinical Centre for Ruminant and Camelid Medicine, University of Veterinary Medicine, 1210 Vienna, AustriaInstitute of Microbiology, University of Veterinary Medicine, 1210 Vienna, AustriaLeibniz Institute of Photonic Technology (IPHT), Leibniz Center for Photonics in Infection Research (LPI), 07745 Jena, GermanyLeibniz Institute of Photonic Technology (IPHT), Leibniz Center for Photonics in Infection Research (LPI), 07745 Jena, GermanyLeibniz Institute of Photonic Technology (IPHT), Leibniz Center for Photonics in Infection Research (LPI), 07745 Jena, GermanyLeibniz Institute of Photonic Technology (IPHT), Leibniz Center for Photonics in Infection Research (LPI), 07745 Jena, GermanyClinical Centre for Ruminant and Camelid Medicine, University of Veterinary Medicine, 1210 Vienna, AustriaIn the field of cattle medicine in Austria, to date, few studies have investigated the presence of methicillin-resistant <i>Staphylococcus aureus</i> and extended-spectrum β-lactamase-producing <i>Escherichia coli</i> in Austria. For this reason, milk and nasal samples were examined for the presence of methicillin-resistant <i>Staphylococcus aureus</i> as well as fecal samples for extended-spectrum cephalosporin-resistant <i>Escherichia coli</i>. The nasal and fecal swabs were collected during the veterinary treatment of calf pneumonia and calf diarrhea. For the milk samples, the first milk jets were milked into a pre-milking cup and then the teats were cleaned and disinfected before the samples were taken. The cows were selected during the veterinary visits to the farms when treatment was necessary due to mastitis. Depending on the severity of the mastitis (acute mastitis or subclinical mastitis), antibiotics and non-steroidal anti-inflammatory drugs were given immediately (acute disease) or after completion of the antibiogram (subclinical disease). Isolates were characterized by a polyphasic approach including susceptibility pheno- and genotyping and microarray-based assays. No methicillin-resistant <i>Staphylococcus aureus</i> was found in the milk samples, but one nasal swab was positive for methicillin-resistant <i>Staphylococcus aureus</i>. Twenty-two <i>Escherichia coli</i> isolates were detected among the fecal samples. All the <i>Escherichia coli</i> isolates were resistant to ceftazidime. In all the <i>Escherichia coli</i> isolates, genes from the <i>bla</i><sub>CTX</sub> family were detected with other <i>bla</i> genes or alone; the most frequently observed β-lactamase gene was <i>bla</i><sub>CTX-M-1/15</sub> (n = 20). In total, 63.6% (n = 14) of the isolates exhibited a multidrug-resistant phenotype and one <i>E. coli</i> isolate (4.5%) harbored the AmpC gene. Precisely because the presence of data regarding extended-spectrum cephalosporin-resistant <i>Escherichia coli</i> and methicillin-resistant <i>Staphylococcus aureus</i> in calves and cows in Austria is rare, this study further expands our understanding of antimicrobial resistance in Austrian cattle, which is highly relevant for successful antibiotic therapy in sick cattle.https://www.mdpi.com/2076-2615/14/23/3383MRSAESBLAmpCcattlebovine<i>E. coli</i> |
| spellingShingle | Lisa Abdank Igor Loncaric Sascha D. Braun Elke Müller Stefan Monecke Ralf Ehricht Reinhild Krametter-Frötscher Characterizing Methicillin-Resistant <i>Staphylococcus</i> spp. and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> in Cattle Animals MRSA ESBL AmpC cattle bovine <i>E. coli</i> |
| title | Characterizing Methicillin-Resistant <i>Staphylococcus</i> spp. and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> in Cattle |
| title_full | Characterizing Methicillin-Resistant <i>Staphylococcus</i> spp. and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> in Cattle |
| title_fullStr | Characterizing Methicillin-Resistant <i>Staphylococcus</i> spp. and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> in Cattle |
| title_full_unstemmed | Characterizing Methicillin-Resistant <i>Staphylococcus</i> spp. and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> in Cattle |
| title_short | Characterizing Methicillin-Resistant <i>Staphylococcus</i> spp. and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> in Cattle |
| title_sort | characterizing methicillin resistant i staphylococcus i spp and extended spectrum cephalosporin resistant i escherichia coli i in cattle |
| topic | MRSA ESBL AmpC cattle bovine <i>E. coli</i> |
| url | https://www.mdpi.com/2076-2615/14/23/3383 |
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