A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis
The stress-associated protein (SAP) multigene family is conserved in both animals and plants. Its function in some animals and plants are known, but it is yet to be deciphered in wheat (Triticum aestivum L.). We identified the wheat gene TaSAP17-D, a member of the SAP gene family with an AN1/AN1 con...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2018-03-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311917616812 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849251068932784128 |
|---|---|
| author | Qiao-fang XU Xin-guo MAO Yi-xue WANG Jing-yi WANG Ya-jun XI Rui-lian JING |
| author_facet | Qiao-fang XU Xin-guo MAO Yi-xue WANG Jing-yi WANG Ya-jun XI Rui-lian JING |
| author_sort | Qiao-fang XU |
| collection | DOAJ |
| description | The stress-associated protein (SAP) multigene family is conserved in both animals and plants. Its function in some animals and plants are known, but it is yet to be deciphered in wheat (Triticum aestivum L.). We identified the wheat gene TaSAP17-D, a member of the SAP gene family with an AN1/AN1 conserved domain. Subcellular localization indicated that TaSAP17-D localized to the nucleus, cytoplasm, and cell membrane. Expression pattern analyses revealed that TaSAP17-D was highly expressed in seedlings and was involved in NaCl response, polyethylene glycol (PEG), cold, and exogenous abscisic acid (ABA). Constitutive expression of TaSAP17-D in transgenic Arabidopsis resulted in enhanced tolerance to salt stress, confirmed by improved multiple physiological indices and significantly upregulated marker genes related to salt stress response. Our results suggest that TaSAP17-D is a candidate gene that can be used to protect crop plants from salt stress. |
| format | Article |
| id | doaj-art-9dce1a0ce1f94bd389f15f2aa677b601 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-9dce1a0ce1f94bd389f15f2aa677b6012025-08-20T03:57:03ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192018-03-0117350751610.1016/S2095-3119(17)61681-2A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic ArabidopsisQiao-fang XU0Xin-guo MAO1Yi-xue WANG2Jing-yi WANG3Ya-jun XI4Rui-lian JING5College of Agronomy, Northwest A&F University, Yangling 712100, P.R.China; National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China; XU Qiao-fang, Mobile: +86-13011216511National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, P.R.China; Correspondence XI Ya-jun, Tel: +86-29-87082845National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China; Correspondence JING Rui-lian, Tel: +86-10-82105829The stress-associated protein (SAP) multigene family is conserved in both animals and plants. Its function in some animals and plants are known, but it is yet to be deciphered in wheat (Triticum aestivum L.). We identified the wheat gene TaSAP17-D, a member of the SAP gene family with an AN1/AN1 conserved domain. Subcellular localization indicated that TaSAP17-D localized to the nucleus, cytoplasm, and cell membrane. Expression pattern analyses revealed that TaSAP17-D was highly expressed in seedlings and was involved in NaCl response, polyethylene glycol (PEG), cold, and exogenous abscisic acid (ABA). Constitutive expression of TaSAP17-D in transgenic Arabidopsis resulted in enhanced tolerance to salt stress, confirmed by improved multiple physiological indices and significantly upregulated marker genes related to salt stress response. Our results suggest that TaSAP17-D is a candidate gene that can be used to protect crop plants from salt stress.http://www.sciencedirect.com/science/article/pii/S2095311917616812SAPmultigene familyTaSAP17-Dsalt tolerancewheat |
| spellingShingle | Qiao-fang XU Xin-guo MAO Yi-xue WANG Jing-yi WANG Ya-jun XI Rui-lian JING A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis Journal of Integrative Agriculture SAP multigene family TaSAP17-D salt tolerance wheat |
| title | A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis |
| title_full | A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis |
| title_fullStr | A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis |
| title_full_unstemmed | A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis |
| title_short | A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis |
| title_sort | wheat gene tasap17 d encoding an an1 an1 zinc finger protein improves salt stress tolerance in transgenic arabidopsis |
| topic | SAP multigene family TaSAP17-D salt tolerance wheat |
| url | http://www.sciencedirect.com/science/article/pii/S2095311917616812 |
| work_keys_str_mv | AT qiaofangxu awheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT xinguomao awheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT yixuewang awheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT jingyiwang awheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT yajunxi awheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT ruilianjing awheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT qiaofangxu wheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT xinguomao wheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT yixuewang wheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT jingyiwang wheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT yajunxi wheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis AT ruilianjing wheatgenetasap17dencodinganan1an1zincfingerproteinimprovessaltstresstoleranceintransgenicarabidopsis |