DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models
Purified subunit viral antigens are weakly immunogenic and stimulate only the antibody but not the T cell-mediated immune response. An alternative approach to inducing protective immunity with small viral peptides may be the targeting of viral epitopes to immunocompetent cells by DNA and protein-eng...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Human Vaccines & Immunotherapeutics |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21645515.2023.2292381 |
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| author | Nikolina M. Mihaylova Iliyan K. Manoylov Maria H. Nikolova Jozsef Prechl Andrey I. Tchorbanov |
| author_facet | Nikolina M. Mihaylova Iliyan K. Manoylov Maria H. Nikolova Jozsef Prechl Andrey I. Tchorbanov |
| author_sort | Nikolina M. Mihaylova |
| collection | DOAJ |
| description | Purified subunit viral antigens are weakly immunogenic and stimulate only the antibody but not the T cell-mediated immune response. An alternative approach to inducing protective immunity with small viral peptides may be the targeting of viral epitopes to immunocompetent cells by DNA and protein-engineered vaccines. This review will focus on DNA and protein-generated chimeric molecules carrying engineered fragments specific for activating cell surface co-receptors for inducing protective antiviral immunity. Adjuvanted protein-based vaccine or DNA constructs encoding simultaneously T- and B-cell peptide epitopes from influenza viral hemagglutinin, and scFvs specific for costimulatory immune cell receptors may induce a significant increase of anti-influenza antibody levels and strong CTL activity against virus-infected cells in a manner that mimics the natural infection. Here we summarize the development of several DNA and protein chimeric constructs carrying influenza virus HA317–41 fragment. The generated engineered molecules were used for immunization in intact murine and experimentally humanized NSG mouse models. |
| format | Article |
| id | doaj-art-9dbda22cac0645fd85ef3d66713f1076 |
| institution | DOAJ |
| issn | 2164-5515 2164-554X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj-art-9dbda22cac0645fd85ef3d66713f10762025-08-20T03:21:46ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2024-12-0120110.1080/21645515.2023.2292381DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer modelsNikolina M. Mihaylova0Iliyan K. Manoylov1Maria H. Nikolova2Jozsef Prechl3Andrey I. Tchorbanov4Laboratory of Experimental Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, BulgariaLaboratory of Experimental Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, BulgariaNational Reference Laboratory of Immunology, National Center of Infectious and Parasitic Diseases, Sofia, BulgariaR&D Laboratory, Diagnosticum zrt, Budapest, HungaryLaboratory of Experimental Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, BulgariaPurified subunit viral antigens are weakly immunogenic and stimulate only the antibody but not the T cell-mediated immune response. An alternative approach to inducing protective immunity with small viral peptides may be the targeting of viral epitopes to immunocompetent cells by DNA and protein-engineered vaccines. This review will focus on DNA and protein-generated chimeric molecules carrying engineered fragments specific for activating cell surface co-receptors for inducing protective antiviral immunity. Adjuvanted protein-based vaccine or DNA constructs encoding simultaneously T- and B-cell peptide epitopes from influenza viral hemagglutinin, and scFvs specific for costimulatory immune cell receptors may induce a significant increase of anti-influenza antibody levels and strong CTL activity against virus-infected cells in a manner that mimics the natural infection. Here we summarize the development of several DNA and protein chimeric constructs carrying influenza virus HA317–41 fragment. The generated engineered molecules were used for immunization in intact murine and experimentally humanized NSG mouse models.https://www.tandfonline.com/doi/10.1080/21645515.2023.2292381DNA vaccinesengineered antibodiesinfluenza viruschimeric molecules |
| spellingShingle | Nikolina M. Mihaylova Iliyan K. Manoylov Maria H. Nikolova Jozsef Prechl Andrey I. Tchorbanov DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models Human Vaccines & Immunotherapeutics DNA vaccines engineered antibodies influenza virus chimeric molecules |
| title | DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models |
| title_full | DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models |
| title_fullStr | DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models |
| title_full_unstemmed | DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models |
| title_short | DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models |
| title_sort | dna and protein generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized nsg transfer models |
| topic | DNA vaccines engineered antibodies influenza virus chimeric molecules |
| url | https://www.tandfonline.com/doi/10.1080/21645515.2023.2292381 |
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