Timing of intermittent seminal HIV-1 RNA shedding in patients with undetectable plasma viral load under combination antiretroviral therapy.
It was demonstrated that combination antiretroviral therapy (cART) reduces the HIV-1 viral load (VL) in the blood and the seminal compartment. Some studies have reported that the seminal HIV-1 VL is undetectable in individuals with an undetectable blood plasma viral load (bpVL) under cART. However,...
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0088922&type=printable |
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| author | Xavier Ferraretto Candice Estellat Florence Damond Pascale Longuet Sylvie Epelboin Pauline Demailly Chadi Yazbeck Marie-Astrid Llabador Blandine Pasquet Yazdan Yazdanpanah Sophie Matheron Catherine Patrat |
| author_facet | Xavier Ferraretto Candice Estellat Florence Damond Pascale Longuet Sylvie Epelboin Pauline Demailly Chadi Yazbeck Marie-Astrid Llabador Blandine Pasquet Yazdan Yazdanpanah Sophie Matheron Catherine Patrat |
| author_sort | Xavier Ferraretto |
| collection | DOAJ |
| description | It was demonstrated that combination antiretroviral therapy (cART) reduces the HIV-1 viral load (VL) in the blood and the seminal compartment. Some studies have reported that the seminal HIV-1 VL is undetectable in individuals with an undetectable blood plasma viral load (bpVL) under cART. However, some recent studies have demonstrated that seminal HIV-1 RNA may still be detected, and potentially infectious, even in the case of an undetectable bpVL. The aim of this retrospective study was to determine the detection rate of a seminal VL and whether shedding could be intermittent over a very short time. From January 2006 to December 2011, 88 HIV-1 infected men, enrolled in an Assisted Reproduction program, provided 306 semen samples, corresponding to 177 frozen sperm samples (two samples delivered at a one-hour interval (n = 129) or one sample (n = 48)). All enrolled men were under cART, with an undetectable bpVL for more than 6 months. HIV-1 RNA was quantified in seminal plasma using a Roche COBAS Ampliprep COBAS TaqMan HIV-1 test. Seminal HIV-1 RNA was detected in 23 samples (7.5%) from 17 patients (19.3%). This detection rate was stable over years. With regards to the freezing of two samples delivered at a one-hour interval, the proportion of discordance between the first and second samples was 9.3% (12/129). Our results confirm the intermittent shedding of HIV-1 in semen. While this finding has been shown by studies examining longer time intervals, to our knowledge, this has never been demonstrated over such a short time interval. |
| format | Article |
| id | doaj-art-9db9095df94c45eaa445e914c57bb5da |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-9db9095df94c45eaa445e914c57bb5da2025-08-20T02:15:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e8892210.1371/journal.pone.0088922Timing of intermittent seminal HIV-1 RNA shedding in patients with undetectable plasma viral load under combination antiretroviral therapy.Xavier FerrarettoCandice EstellatFlorence DamondPascale LonguetSylvie EpelboinPauline DemaillyChadi YazbeckMarie-Astrid LlabadorBlandine PasquetYazdan YazdanpanahSophie MatheronCatherine PatratIt was demonstrated that combination antiretroviral therapy (cART) reduces the HIV-1 viral load (VL) in the blood and the seminal compartment. Some studies have reported that the seminal HIV-1 VL is undetectable in individuals with an undetectable blood plasma viral load (bpVL) under cART. However, some recent studies have demonstrated that seminal HIV-1 RNA may still be detected, and potentially infectious, even in the case of an undetectable bpVL. The aim of this retrospective study was to determine the detection rate of a seminal VL and whether shedding could be intermittent over a very short time. From January 2006 to December 2011, 88 HIV-1 infected men, enrolled in an Assisted Reproduction program, provided 306 semen samples, corresponding to 177 frozen sperm samples (two samples delivered at a one-hour interval (n = 129) or one sample (n = 48)). All enrolled men were under cART, with an undetectable bpVL for more than 6 months. HIV-1 RNA was quantified in seminal plasma using a Roche COBAS Ampliprep COBAS TaqMan HIV-1 test. Seminal HIV-1 RNA was detected in 23 samples (7.5%) from 17 patients (19.3%). This detection rate was stable over years. With regards to the freezing of two samples delivered at a one-hour interval, the proportion of discordance between the first and second samples was 9.3% (12/129). Our results confirm the intermittent shedding of HIV-1 in semen. While this finding has been shown by studies examining longer time intervals, to our knowledge, this has never been demonstrated over such a short time interval.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0088922&type=printable |
| spellingShingle | Xavier Ferraretto Candice Estellat Florence Damond Pascale Longuet Sylvie Epelboin Pauline Demailly Chadi Yazbeck Marie-Astrid Llabador Blandine Pasquet Yazdan Yazdanpanah Sophie Matheron Catherine Patrat Timing of intermittent seminal HIV-1 RNA shedding in patients with undetectable plasma viral load under combination antiretroviral therapy. PLoS ONE |
| title | Timing of intermittent seminal HIV-1 RNA shedding in patients with undetectable plasma viral load under combination antiretroviral therapy. |
| title_full | Timing of intermittent seminal HIV-1 RNA shedding in patients with undetectable plasma viral load under combination antiretroviral therapy. |
| title_fullStr | Timing of intermittent seminal HIV-1 RNA shedding in patients with undetectable plasma viral load under combination antiretroviral therapy. |
| title_full_unstemmed | Timing of intermittent seminal HIV-1 RNA shedding in patients with undetectable plasma viral load under combination antiretroviral therapy. |
| title_short | Timing of intermittent seminal HIV-1 RNA shedding in patients with undetectable plasma viral load under combination antiretroviral therapy. |
| title_sort | timing of intermittent seminal hiv 1 rna shedding in patients with undetectable plasma viral load under combination antiretroviral therapy |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0088922&type=printable |
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