Wharton's jelly stem cells delivered via a curcumin-loaded nanofibrous wound dressings improved diabetic wound healing via upregulating VEGF and IGF genes: An in vitro and in vivo study
Diabetic wounds pose an enduring clinical hurdle, marked by delayed recovery, persistent inflammation, and an elevated susceptibility to infections. Conventional treatment approaches often fall short of delivering optimal outcomes, prompting the exploration of innovative methods to enhance the heali...
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Elsevier
2024-06-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320424001263 |
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| author | Chengjin Chen Hui Zhao Wenlu Zhang Xuelan Hong Shengjie Li Saeed Rohani |
| author_facet | Chengjin Chen Hui Zhao Wenlu Zhang Xuelan Hong Shengjie Li Saeed Rohani |
| author_sort | Chengjin Chen |
| collection | DOAJ |
| description | Diabetic wounds pose an enduring clinical hurdle, marked by delayed recovery, persistent inflammation, and an elevated susceptibility to infections. Conventional treatment approaches often fall short of delivering optimal outcomes, prompting the exploration of innovative methods to enhance the healing process. Electrospun wound dressings offer superior healing, controlled drug release, enhanced cell proliferation, biocompatibility, high surface area, and antimicrobial properties. In the current study, polycaprolactone/gelatin-based nanofibrous wound dressings were developed for the delivery of Wharton's jelly stem cells and curcumin into the diabetic wounds bed. Curcumin was loaded into the polycaprolactone/gelatin solution and electrospun to produce curcumin-loaded scaffolds. In vitro experiments including scanning electron microscopy, cell viability assay, release assay, hemocompatibility assay, cell proliferation assay, and antibacterial assay were utilized to characterize the delivery system. Then, curcumin-loaded scaffolds were seeded with 30,000 Wharton's jelly stem cells and implanted into a rat model of diabetic wounds. Study showed that the scaffolds containing both Wharton's jelly stem cells and curcumin significantly improved diabetic wound closure (86.32 3.88% at the end of 14th day), augmented collagen deposition, and improved epithelial tissue formation. Gene expression studies showed that VEGF and IGF genes were significantly upregulated by the co-delivery system. Our developed system may have augmented diabetic wound healing via upregulating pro-healing genes. |
| format | Article |
| id | doaj-art-9db0a53c529c44ddbd0c0786b1633ff3 |
| institution | OA Journals |
| issn | 2352-3204 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-9db0a53c529c44ddbd0c0786b1633ff32025-08-20T02:07:34ZengElsevierRegenerative Therapy2352-32042024-06-012654755610.1016/j.reth.2024.06.018Wharton's jelly stem cells delivered via a curcumin-loaded nanofibrous wound dressings improved diabetic wound healing via upregulating VEGF and IGF genes: An in vitro and in vivo studyChengjin Chen0Hui Zhao1Wenlu Zhang2Xuelan Hong3Shengjie Li4Saeed Rohani5Department of Orthopedics and Traumatology, The Third People's Hospital of Yunnan Province, Kunming City, 650000, ChinaDepartment of Orthopedics and Traumatology, The Third People's Hospital of Yunnan Province, Kunming City, 650000, ChinaRehabilitation Medicine Department, The Third People's Hospital of Yunnan Province, Kunming City, 650000, ChinaDepartment of Orthopedics and Traumatology, The Third People's Hospital of Yunnan Province, Kunming City, 650000, China; Corresponding author.Department of Orthopedics and Traumatology, The Third People's Hospital of Yunnan Province, Kunming City, 650000, China; Corresponding author.Department of Tissue Engineering and Applied Cell Science, Tehran University of Medical Sciences, Tehran, 444522125, IranDiabetic wounds pose an enduring clinical hurdle, marked by delayed recovery, persistent inflammation, and an elevated susceptibility to infections. Conventional treatment approaches often fall short of delivering optimal outcomes, prompting the exploration of innovative methods to enhance the healing process. Electrospun wound dressings offer superior healing, controlled drug release, enhanced cell proliferation, biocompatibility, high surface area, and antimicrobial properties. In the current study, polycaprolactone/gelatin-based nanofibrous wound dressings were developed for the delivery of Wharton's jelly stem cells and curcumin into the diabetic wounds bed. Curcumin was loaded into the polycaprolactone/gelatin solution and electrospun to produce curcumin-loaded scaffolds. In vitro experiments including scanning electron microscopy, cell viability assay, release assay, hemocompatibility assay, cell proliferation assay, and antibacterial assay were utilized to characterize the delivery system. Then, curcumin-loaded scaffolds were seeded with 30,000 Wharton's jelly stem cells and implanted into a rat model of diabetic wounds. Study showed that the scaffolds containing both Wharton's jelly stem cells and curcumin significantly improved diabetic wound closure (86.32 3.88% at the end of 14th day), augmented collagen deposition, and improved epithelial tissue formation. Gene expression studies showed that VEGF and IGF genes were significantly upregulated by the co-delivery system. Our developed system may have augmented diabetic wound healing via upregulating pro-healing genes.http://www.sciencedirect.com/science/article/pii/S2352320424001263Diabetic woundsEndocrine systemWound healingNanofibersWound dressing |
| spellingShingle | Chengjin Chen Hui Zhao Wenlu Zhang Xuelan Hong Shengjie Li Saeed Rohani Wharton's jelly stem cells delivered via a curcumin-loaded nanofibrous wound dressings improved diabetic wound healing via upregulating VEGF and IGF genes: An in vitro and in vivo study Regenerative Therapy Diabetic wounds Endocrine system Wound healing Nanofibers Wound dressing |
| title | Wharton's jelly stem cells delivered via a curcumin-loaded nanofibrous wound dressings improved diabetic wound healing via upregulating VEGF and IGF genes: An in vitro and in vivo study |
| title_full | Wharton's jelly stem cells delivered via a curcumin-loaded nanofibrous wound dressings improved diabetic wound healing via upregulating VEGF and IGF genes: An in vitro and in vivo study |
| title_fullStr | Wharton's jelly stem cells delivered via a curcumin-loaded nanofibrous wound dressings improved diabetic wound healing via upregulating VEGF and IGF genes: An in vitro and in vivo study |
| title_full_unstemmed | Wharton's jelly stem cells delivered via a curcumin-loaded nanofibrous wound dressings improved diabetic wound healing via upregulating VEGF and IGF genes: An in vitro and in vivo study |
| title_short | Wharton's jelly stem cells delivered via a curcumin-loaded nanofibrous wound dressings improved diabetic wound healing via upregulating VEGF and IGF genes: An in vitro and in vivo study |
| title_sort | wharton s jelly stem cells delivered via a curcumin loaded nanofibrous wound dressings improved diabetic wound healing via upregulating vegf and igf genes an in vitro and in vivo study |
| topic | Diabetic wounds Endocrine system Wound healing Nanofibers Wound dressing |
| url | http://www.sciencedirect.com/science/article/pii/S2352320424001263 |
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