Ontological differences in first compared to third trimester human fetal placental chorionic stem cells.
Human mesenchymal stromal/stem cells (MSC) isolated from fetal tissues hold promise for use in tissue engineering applications and cell-based therapies, but their collection is restricted ethically and technically. In contrast, the placenta is a potential source of readily-obtainable stem cells thro...
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0043395&type=printable |
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| author | Gemma N Jones Dafni Moschidou Tamara-Isabel Puga-Iglesias Katarzyna Kuleszewicz Maximilien Vanleene Sandra J Shefelbine George Bou-Gharios Nicholas M Fisk Anna L David Paolo De Coppi Pascale V Guillot |
| author_facet | Gemma N Jones Dafni Moschidou Tamara-Isabel Puga-Iglesias Katarzyna Kuleszewicz Maximilien Vanleene Sandra J Shefelbine George Bou-Gharios Nicholas M Fisk Anna L David Paolo De Coppi Pascale V Guillot |
| author_sort | Gemma N Jones |
| collection | DOAJ |
| description | Human mesenchymal stromal/stem cells (MSC) isolated from fetal tissues hold promise for use in tissue engineering applications and cell-based therapies, but their collection is restricted ethically and technically. In contrast, the placenta is a potential source of readily-obtainable stem cells throughout pregnancy. In fetal tissues, early gestational stem cells are known to have advantageous characteristics over neonatal and adult stem cells. Accordingly, we investigated whether early fetal placental chorionic stem cells (e-CSC) were physiologically superior to their late gestation fetal chorionic counterparts (l-CSC). We showed that e-CSC shared a common phenotype with l-CSC, differentiating down the osteogenic, adipogenic and neurogenic pathways, and containing a subset of cells endogenously expressing NANOG, SOX2, c-MYC, and KLF4, as well as an array of genes expressed in pluripotent stem cells and primordial germ cells, including CD24, NANOG, SSEA4, SSEA3, TRA-1-60, TRA-1-81, STELLA, FRAGILIS, NANOS3, DAZL and SSEA1. However, we showed that e-CSC have characteristics of an earlier state of stemness compared to l-CSC, such as smaller size, faster kinetics, uniquely expressing OCT4A variant 1 and showing higher levels of expression of NANOG, SOX2, c-MYC and KLF4 than l-CSC. Furthermore e-CSC, but not l-CSC, formed embryoid bodies containing cells from the three germ layer lineages. Finally, we showed that e-CSC demonstrate higher tissue repair in vivo; when transplanted in the osteogenesis imperfecta mice, e-CSC, but not l-CSC increased bone quality and plasticity; and when applied to a skin wound, e-CSC, but not l-CSC, accelerated healing compared to controls. Our results provide insight into the ontogeny of the stemness phenotype during fetal development and suggest that the more primitive characteristics of early compared to late gestation fetal chorionic stem cells may be translationally advantageous. |
| format | Article |
| id | doaj-art-9daf098975aa48ffa18a8f9bc460f3f2 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-9daf098975aa48ffa18a8f9bc460f3f22025-08-20T03:22:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4339510.1371/journal.pone.0043395Ontological differences in first compared to third trimester human fetal placental chorionic stem cells.Gemma N JonesDafni MoschidouTamara-Isabel Puga-IglesiasKatarzyna KuleszewiczMaximilien VanleeneSandra J ShefelbineGeorge Bou-GhariosNicholas M FiskAnna L DavidPaolo De CoppiPascale V GuillotHuman mesenchymal stromal/stem cells (MSC) isolated from fetal tissues hold promise for use in tissue engineering applications and cell-based therapies, but their collection is restricted ethically and technically. In contrast, the placenta is a potential source of readily-obtainable stem cells throughout pregnancy. In fetal tissues, early gestational stem cells are known to have advantageous characteristics over neonatal and adult stem cells. Accordingly, we investigated whether early fetal placental chorionic stem cells (e-CSC) were physiologically superior to their late gestation fetal chorionic counterparts (l-CSC). We showed that e-CSC shared a common phenotype with l-CSC, differentiating down the osteogenic, adipogenic and neurogenic pathways, and containing a subset of cells endogenously expressing NANOG, SOX2, c-MYC, and KLF4, as well as an array of genes expressed in pluripotent stem cells and primordial germ cells, including CD24, NANOG, SSEA4, SSEA3, TRA-1-60, TRA-1-81, STELLA, FRAGILIS, NANOS3, DAZL and SSEA1. However, we showed that e-CSC have characteristics of an earlier state of stemness compared to l-CSC, such as smaller size, faster kinetics, uniquely expressing OCT4A variant 1 and showing higher levels of expression of NANOG, SOX2, c-MYC and KLF4 than l-CSC. Furthermore e-CSC, but not l-CSC, formed embryoid bodies containing cells from the three germ layer lineages. Finally, we showed that e-CSC demonstrate higher tissue repair in vivo; when transplanted in the osteogenesis imperfecta mice, e-CSC, but not l-CSC increased bone quality and plasticity; and when applied to a skin wound, e-CSC, but not l-CSC, accelerated healing compared to controls. Our results provide insight into the ontogeny of the stemness phenotype during fetal development and suggest that the more primitive characteristics of early compared to late gestation fetal chorionic stem cells may be translationally advantageous.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0043395&type=printable |
| spellingShingle | Gemma N Jones Dafni Moschidou Tamara-Isabel Puga-Iglesias Katarzyna Kuleszewicz Maximilien Vanleene Sandra J Shefelbine George Bou-Gharios Nicholas M Fisk Anna L David Paolo De Coppi Pascale V Guillot Ontological differences in first compared to third trimester human fetal placental chorionic stem cells. PLoS ONE |
| title | Ontological differences in first compared to third trimester human fetal placental chorionic stem cells. |
| title_full | Ontological differences in first compared to third trimester human fetal placental chorionic stem cells. |
| title_fullStr | Ontological differences in first compared to third trimester human fetal placental chorionic stem cells. |
| title_full_unstemmed | Ontological differences in first compared to third trimester human fetal placental chorionic stem cells. |
| title_short | Ontological differences in first compared to third trimester human fetal placental chorionic stem cells. |
| title_sort | ontological differences in first compared to third trimester human fetal placental chorionic stem cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0043395&type=printable |
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