Influence of Marsilea crenata and Alpinia purpurata Ethanol Extract on MDA and SOD Testicular Cells of Hyperglycemia Mice

Hyperglycemia can induce testicular damage that leads to male infertility. Herbal plants, such as Marsilea crenata and Alpinia purpurata, are used for antioxidant defense systems to repair reproductive disorders due to hyperglycemia. This study aims to evaluate the effect of M. crenata and A. purpur...

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Main Authors: Febriane Eka Damayanti, Sri Rahayu, Sri Widyarti
Format: Article
Language:English
Published: University of Brawijaya 2023-10-01
Series:Journal of Experimental Life Science
Subjects:
Online Access:https://jels.ub.ac.id/index.php/jels/article/view/539
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author Febriane Eka Damayanti
Sri Rahayu
Sri Widyarti
author_facet Febriane Eka Damayanti
Sri Rahayu
Sri Widyarti
author_sort Febriane Eka Damayanti
collection DOAJ
description Hyperglycemia can induce testicular damage that leads to male infertility. Herbal plants, such as Marsilea crenata and Alpinia purpurata, are used for antioxidant defense systems to repair reproductive disorders due to hyperglycemia. This study aims to evaluate the effect of M. crenata and A. purpurata ethanol extracts on MDA and SOD testes of hyperglycemia mice. This study used a completely randomized design with seven treatment groups (n=4), namely N (control group), H (hyperglycemia mice), Met (hyperglycemia mice given metformin), D1 (0. 09 mg.g-1 M. crenata), D2 (0.2 mg.g-1 A. purpurata), D3 (0.09 mg.g-1 M. crenata + 0.2 mg.g-1 A. purpurata), and D4 (0.09 mg.g-1 M. crenata + 0.4 mg.g-1 A. purpurata). The extracts were administered orally for 17 days. Analysis of testicular MDA and SOD levels was performed by flow cytometry. Data analysis was performed with a one-way ANOVA test and continued with the Tukey test. The results showed that the D4 treatment group, compared to D3, D2, D1, Met, H, and N, showed a better decrease in MDA levels (4.47%) and an increase in SOD levels in the D4 group (4.77%). The research concludes that the combination of 0.09 mg.g-1 M. crenata and 0.4 mg.g-1 A. purpurata was an optimal dose to decrease MDA levels and increase SOD levels in the testes of hyperglycemic mice.
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spelling doaj-art-9dacc1da34a3469b9f539d95c23cdd062025-08-20T01:48:20ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552023-10-01133159165https://doi.org/10.21776/ub.jels.2023.013.03.04Influence of Marsilea crenata and Alpinia purpurata Ethanol Extract on MDA and SOD Testicular Cells of Hyperglycemia MiceFebriane Eka Damayanti0Sri Rahayu1Sri Widyarti2Universitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaHyperglycemia can induce testicular damage that leads to male infertility. Herbal plants, such as Marsilea crenata and Alpinia purpurata, are used for antioxidant defense systems to repair reproductive disorders due to hyperglycemia. This study aims to evaluate the effect of M. crenata and A. purpurata ethanol extracts on MDA and SOD testes of hyperglycemia mice. This study used a completely randomized design with seven treatment groups (n=4), namely N (control group), H (hyperglycemia mice), Met (hyperglycemia mice given metformin), D1 (0. 09 mg.g-1 M. crenata), D2 (0.2 mg.g-1 A. purpurata), D3 (0.09 mg.g-1 M. crenata + 0.2 mg.g-1 A. purpurata), and D4 (0.09 mg.g-1 M. crenata + 0.4 mg.g-1 A. purpurata). The extracts were administered orally for 17 days. Analysis of testicular MDA and SOD levels was performed by flow cytometry. Data analysis was performed with a one-way ANOVA test and continued with the Tukey test. The results showed that the D4 treatment group, compared to D3, D2, D1, Met, H, and N, showed a better decrease in MDA levels (4.47%) and an increase in SOD levels in the D4 group (4.77%). The research concludes that the combination of 0.09 mg.g-1 M. crenata and 0.4 mg.g-1 A. purpurata was an optimal dose to decrease MDA levels and increase SOD levels in the testes of hyperglycemic mice.https://jels.ub.ac.id/index.php/jels/article/view/539alpinia purpuratamarsilea crenatamdasodtestes.
spellingShingle Febriane Eka Damayanti
Sri Rahayu
Sri Widyarti
Influence of Marsilea crenata and Alpinia purpurata Ethanol Extract on MDA and SOD Testicular Cells of Hyperglycemia Mice
Journal of Experimental Life Science
alpinia purpurata
marsilea crenata
mda
sod
testes.
title Influence of Marsilea crenata and Alpinia purpurata Ethanol Extract on MDA and SOD Testicular Cells of Hyperglycemia Mice
title_full Influence of Marsilea crenata and Alpinia purpurata Ethanol Extract on MDA and SOD Testicular Cells of Hyperglycemia Mice
title_fullStr Influence of Marsilea crenata and Alpinia purpurata Ethanol Extract on MDA and SOD Testicular Cells of Hyperglycemia Mice
title_full_unstemmed Influence of Marsilea crenata and Alpinia purpurata Ethanol Extract on MDA and SOD Testicular Cells of Hyperglycemia Mice
title_short Influence of Marsilea crenata and Alpinia purpurata Ethanol Extract on MDA and SOD Testicular Cells of Hyperglycemia Mice
title_sort influence of marsilea crenata and alpinia purpurata ethanol extract on mda and sod testicular cells of hyperglycemia mice
topic alpinia purpurata
marsilea crenata
mda
sod
testes.
url https://jels.ub.ac.id/index.php/jels/article/view/539
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