In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts
The aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast culture. Cells (3×104 cells/cm2) were seeded on 24-well acrylic plates using plain DMEM supplemented with 10% fetal bovine serum. After 48-hou...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2012/719452 |
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| author | Fernanda G. Basso Taisa N. Pansani Ana Paula S. Turrioni Vanderlei S. Bagnato Josimeri Hebling Carlos A. de Souza Costa |
| author_facet | Fernanda G. Basso Taisa N. Pansani Ana Paula S. Turrioni Vanderlei S. Bagnato Josimeri Hebling Carlos A. de Souza Costa |
| author_sort | Fernanda G. Basso |
| collection | DOAJ |
| description | The aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast culture. Cells (3×104 cells/cm2) were seeded on 24-well acrylic plates using plain DMEM supplemented with 10% fetal bovine serum. After 48-hour incubation with 5% CO2 at 37°C, cells were irradiated with a InGaAsP diode laser prototype (LASERTable; 780±3 nm; 40 mW) with energy doses of 0.5, 1.5, 3, 5, and 7 J/cm2. Cells were irradiated every 24 h totalizing 3 applications. Twenty-four hours after the last irradiation, cell metabolism was evaluated by the MTT assay and the two most effective doses (0.5 and 3 J/cm2) were selected to evaluate the cell number (trypan blue assay) and the cell migration capacity (wound healing assay; transwell migration assay). Data were analyzed by the Kruskal-Wallis and Mann-Whitney nonparametric tests with statistical significance of 5%. Irradiation of the fibroblasts with 0.5 and 3 J/cm2 resulted in significant increase in cell metabolism compared with the nonrradiated group (P<0.05). Both energy doses promoted significant increase in the cell number as well as in cell migration (P<0.05). These results demonstrate that, under the tested conditions, LLLT promoted biostimulation of fibroblasts in vitro. |
| format | Article |
| id | doaj-art-9d9d61fa7c674481abff0a63ee6b8341 |
| institution | OA Journals |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-9d9d61fa7c674481abff0a63ee6b83412025-08-20T02:20:29ZengWileyInternational Journal of Dentistry1687-87281687-87362012-01-01201210.1155/2012/719452719452In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival FibroblastsFernanda G. Basso0Taisa N. Pansani1Ana Paula S. Turrioni2Vanderlei S. Bagnato3Josimeri Hebling4Carlos A. de Souza Costa5Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (UNICAMP), 13414-903 Piracicaba, SP, BrazilFaculdade de Odontologia de Araraquara, Universidade de Estadual Paulista (UNESP), 14801-903 Araraquara, SP, BrazilFaculdade de Odontologia de Araraquara, Universidade de Estadual Paulista (UNESP), 14801-903 Araraquara, SP, BrazilInstituto de Física de São Carlos, Universidade de São Paulo (USP), 13560-970 São Carlos, SP, BrazilFaculdade de Odontologia de Araraquara, Universidade de Estadual Paulista (UNESP), 14801-903 Araraquara, SP, BrazilFaculdade de Odontologia de Araraquara, Universidade de Estadual Paulista (UNESP), 14801-903 Araraquara, SP, BrazilThe aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast culture. Cells (3×104 cells/cm2) were seeded on 24-well acrylic plates using plain DMEM supplemented with 10% fetal bovine serum. After 48-hour incubation with 5% CO2 at 37°C, cells were irradiated with a InGaAsP diode laser prototype (LASERTable; 780±3 nm; 40 mW) with energy doses of 0.5, 1.5, 3, 5, and 7 J/cm2. Cells were irradiated every 24 h totalizing 3 applications. Twenty-four hours after the last irradiation, cell metabolism was evaluated by the MTT assay and the two most effective doses (0.5 and 3 J/cm2) were selected to evaluate the cell number (trypan blue assay) and the cell migration capacity (wound healing assay; transwell migration assay). Data were analyzed by the Kruskal-Wallis and Mann-Whitney nonparametric tests with statistical significance of 5%. Irradiation of the fibroblasts with 0.5 and 3 J/cm2 resulted in significant increase in cell metabolism compared with the nonrradiated group (P<0.05). Both energy doses promoted significant increase in the cell number as well as in cell migration (P<0.05). These results demonstrate that, under the tested conditions, LLLT promoted biostimulation of fibroblasts in vitro.http://dx.doi.org/10.1155/2012/719452 |
| spellingShingle | Fernanda G. Basso Taisa N. Pansani Ana Paula S. Turrioni Vanderlei S. Bagnato Josimeri Hebling Carlos A. de Souza Costa In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts International Journal of Dentistry |
| title | In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts |
| title_full | In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts |
| title_fullStr | In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts |
| title_full_unstemmed | In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts |
| title_short | In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts |
| title_sort | in vitro wound healing improvement by low level laser therapy application in cultured gingival fibroblasts |
| url | http://dx.doi.org/10.1155/2012/719452 |
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