Temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized 13C MRSI thermometry

Abstract The chemical shift of many molecules changes with temperature, which enables non-invasive magnetic resonance imaging (MRI) thermometry. Hyperpolarization methods increase the inherently low 13C MR signal. The commonly-used hyperpolarized probe [1-13C]pyruvate, and its metabolic product [1-1...

Full description

Saved in:
Bibliographic Details
Main Authors: Wolfgang Gottwald, Luca Nagel, Jason G. Skinner, Martin Grashei, Sandra Sühnel, Nadine Setzer, Wolfgang Eisenreich, Mary A. McLean, Ferdia A. Gallagher, Jae Mo Park, Zumrud Ahmadova, Martin Gierse, Senay Karaali, Stephan Knecht, Ilai Schwartz, Irina Heid, Geoffrey J. Topping, Frits H. A. van Heijster, Franz Schilling
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:npj Imaging
Online Access:https://doi.org/10.1038/s44303-025-00081-3
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849312969325805568
author Wolfgang Gottwald
Luca Nagel
Jason G. Skinner
Martin Grashei
Sandra Sühnel
Nadine Setzer
Wolfgang Eisenreich
Mary A. McLean
Ferdia A. Gallagher
Jae Mo Park
Zumrud Ahmadova
Martin Gierse
Senay Karaali
Stephan Knecht
Ilai Schwartz
Irina Heid
Geoffrey J. Topping
Frits H. A. van Heijster
Franz Schilling
author_facet Wolfgang Gottwald
Luca Nagel
Jason G. Skinner
Martin Grashei
Sandra Sühnel
Nadine Setzer
Wolfgang Eisenreich
Mary A. McLean
Ferdia A. Gallagher
Jae Mo Park
Zumrud Ahmadova
Martin Gierse
Senay Karaali
Stephan Knecht
Ilai Schwartz
Irina Heid
Geoffrey J. Topping
Frits H. A. van Heijster
Franz Schilling
author_sort Wolfgang Gottwald
collection DOAJ
description Abstract The chemical shift of many molecules changes with temperature, which enables non-invasive magnetic resonance imaging (MRI) thermometry. Hyperpolarization methods increase the inherently low 13C MR signal. The commonly-used hyperpolarized probe [1-13C]pyruvate, and its metabolic product [1-13C]lactate, exhibit temperature and concentration dependent chemical shift changes that have not previously been reported. These effects were characterized at 7 T and 11.7 T in vitro and applied for in vivo thermometry both preclinically at 7 T and to human data at 3 T. Apparent temperature values from mouse abdomen and brain were similar to rectally measured temperature. Human brain and kidney apparent temperatures from 13C MRSI were lower than known physiological temperatures, suggesting that additional effects may currently limit the use of this method for determining absolute temperature in humans. The temperature dependent chemical shift changes also have implications for sequence design and for in vitro studies with hyperpolarized pyruvate.
format Article
id doaj-art-9d99f05871cc4cb092891fe983a458ea
institution Kabale University
issn 2948-197X
language English
publishDate 2025-05-01
publisher Nature Portfolio
record_format Article
series npj Imaging
spelling doaj-art-9d99f05871cc4cb092891fe983a458ea2025-08-20T03:52:55ZengNature Portfolionpj Imaging2948-197X2025-05-013111110.1038/s44303-025-00081-3Temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized 13C MRSI thermometryWolfgang Gottwald0Luca Nagel1Jason G. Skinner2Martin Grashei3Sandra Sühnel4Nadine Setzer5Wolfgang Eisenreich6Mary A. McLean7Ferdia A. Gallagher8Jae Mo Park9Zumrud Ahmadova10Martin Gierse11Senay Karaali12Stephan Knecht13Ilai Schwartz14Irina Heid15Geoffrey J. Topping16Frits H. A. van Heijster17Franz Schilling18Department of Nuclear Medicine, TUM School of Medicine and Health, TUM University Hospital, Technical University of MunichDepartment of Nuclear Medicine, TUM School of Medicine and Health, TUM University Hospital, Technical University of MunichDepartment of Nuclear Medicine, TUM School of Medicine and Health, TUM University Hospital, Technical University of MunichDepartment of Nuclear Medicine, TUM School of Medicine and Health, TUM University Hospital, Technical University of MunichDepartment of Nuclear Medicine, TUM School of Medicine and Health, TUM University Hospital, Technical University of MunichDepartment of Nuclear Medicine, TUM School of Medicine and Health, TUM University Hospital, Technical University of MunichBavarian NMR Center (BNMRZ), Structural Membrane Biochemistry, TUM School of Natural Sciences, Technical University of MunichCancer Research UK Cambridge Centre, University of CambridgeCancer Research UK Cambridge Centre, University of CambridgeAdvanced Imaging Research Center, University of Texas Southwestern Medical CenterNVision Imaging Technologies GmbHNVision Imaging Technologies GmbHNVision Imaging Technologies GmbHNVision Imaging Technologies GmbHNVision Imaging Technologies GmbHInstitute of Diagnostic and Interventional Radiology, TUM School of Medicine and Health, TUM University Hospital, Technical University of MunichDepartment of Nuclear Medicine, TUM School of Medicine and Health, TUM University Hospital, Technical University of MunichDepartment of Nuclear Medicine, TUM School of Medicine and Health, TUM University Hospital, Technical University of MunichDepartment of Nuclear Medicine, TUM School of Medicine and Health, TUM University Hospital, Technical University of MunichAbstract The chemical shift of many molecules changes with temperature, which enables non-invasive magnetic resonance imaging (MRI) thermometry. Hyperpolarization methods increase the inherently low 13C MR signal. The commonly-used hyperpolarized probe [1-13C]pyruvate, and its metabolic product [1-13C]lactate, exhibit temperature and concentration dependent chemical shift changes that have not previously been reported. These effects were characterized at 7 T and 11.7 T in vitro and applied for in vivo thermometry both preclinically at 7 T and to human data at 3 T. Apparent temperature values from mouse abdomen and brain were similar to rectally measured temperature. Human brain and kidney apparent temperatures from 13C MRSI were lower than known physiological temperatures, suggesting that additional effects may currently limit the use of this method for determining absolute temperature in humans. The temperature dependent chemical shift changes also have implications for sequence design and for in vitro studies with hyperpolarized pyruvate.https://doi.org/10.1038/s44303-025-00081-3
spellingShingle Wolfgang Gottwald
Luca Nagel
Jason G. Skinner
Martin Grashei
Sandra Sühnel
Nadine Setzer
Wolfgang Eisenreich
Mary A. McLean
Ferdia A. Gallagher
Jae Mo Park
Zumrud Ahmadova
Martin Gierse
Senay Karaali
Stephan Knecht
Ilai Schwartz
Irina Heid
Geoffrey J. Topping
Frits H. A. van Heijster
Franz Schilling
Temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized 13C MRSI thermometry
npj Imaging
title Temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized 13C MRSI thermometry
title_full Temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized 13C MRSI thermometry
title_fullStr Temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized 13C MRSI thermometry
title_full_unstemmed Temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized 13C MRSI thermometry
title_short Temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized 13C MRSI thermometry
title_sort temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized 13c mrsi thermometry
url https://doi.org/10.1038/s44303-025-00081-3
work_keys_str_mv AT wolfganggottwald temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT lucanagel temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT jasongskinner temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT martingrashei temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT sandrasuhnel temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT nadinesetzer temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT wolfgangeisenreich temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT maryamclean temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT ferdiaagallagher temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT jaemopark temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT zumrudahmadova temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT martingierse temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT senaykaraali temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT stephanknecht temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT ilaischwartz temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT irinaheid temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT geoffreyjtopping temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT fritshavanheijster temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry
AT franzschilling temperaturedependentchemicalshiftsofpyruvateandlactateenableinvivohyperpolarized13cmrsithermometry