Development of a quantitative PMA-16S rRNA gene sequencing workflow for absolute abundance measurements of seawater microbial communities
Abstract Background Ecological risk assessments rarely consider the impacts of environmental stress on microbial communities. Incorporating microbial community responses into these evaluations requires establishing sensitivity thresholds based on the absolute abundance of viable taxa. While essentia...
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| Main Authors: | Marie C. Thomas, Gretel Waugh, Katarina Damjanovic, Inka Vanwonterghem, Nicole S. Webster, Andrew P. Negri, Heidi M. Luter |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | Environmental Microbiome |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40793-025-00741-2 |
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