Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece
Extended-spectrum-β-lactamase (ESBL) and carbapenemase-producing <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i> and <i>Acinetobacter</i> spp. are associated with hospital-acquired infections and are commonly isolated across the poultry food production chain...
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2025-01-01
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| author | Anestis Tsitsos Alexandros Damianos Konstantinos Kiskinis Vasilios Tsiouris Ilias Tirodimos Nikolaos Soultos Anna Papa Vangelis Economou |
| author_facet | Anestis Tsitsos Alexandros Damianos Konstantinos Kiskinis Vasilios Tsiouris Ilias Tirodimos Nikolaos Soultos Anna Papa Vangelis Economou |
| author_sort | Anestis Tsitsos |
| collection | DOAJ |
| description | Extended-spectrum-β-lactamase (ESBL) and carbapenemase-producing <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i> and <i>Acinetobacter</i> spp. are associated with hospital-acquired infections and are commonly isolated across the poultry food production chain. Comprehensive data regarding the prevalence, spatiotemporal variations, and characterization of β-lactam-resistant bacteria in poultry farms and slaughterhouses is scarce. This study examines the prevalence and characteristics of β-lactam-resistant <i>E. coli</i>, <i>K. pneumoniae</i>, and <i>Acinetobacter</i> spp. isolated from poultry farms, slaughterhouses, and associated personnel in Greece. Strains were selectively isolated and identified via MALDI-TOF MS, which was also employed to identify possible relatedness. <i>E. coli</i> isolates were further classified into phylogenetic groups. The prevalence of β-lactam-resistant strains in farm and slaughterhouse environments was 15.0% (n = 15 strains)/57.3% (n = 71 strains) for <i>E. coli</i>, 11.0% (n = 11 strains)/1.6% (n = 2 strains) for <i>K. pneumoniae</i>, and 1.0% (n = 1 strain)/25.8% (n = 38 strains) for <i>Acinetobacter</i> spp., respectively. The prevalence of <i>Acinetobacter</i> spp. and <i>E. coli</i> on farmers’ skin was 16.7% (n = 2 strains) and 8.3% (n = 1 strain), correspondingly. Significantly higher <i>E. coli</i> isolation rates were observed in warmer seasons. All strains were multidrug-resistant and most carried ESBL/AmpC genes. Most <i>E. coli</i> isolates belonged to phylogroups A (41.4%, n = 36) and B1 (24.1%, n = 21). Proteomic analysis indicated relatedness among strains from different regions and seasons. Thus, poultry farms and slaughterhouses may serve as significant reservoirs of β-lactam-resistant strains of <i>E. coli</i>, <i>K. pneumoniae</i>, and <i>Acinetobacter</i> spp. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Foods |
| spelling | doaj-art-9d9161f47e764712a31b11e517215cec2025-01-24T13:32:56ZengMDPI AGFoods2304-81582025-01-0114222410.3390/foods14020224Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in GreeceAnestis Tsitsos0Alexandros Damianos1Konstantinos Kiskinis2Vasilios Tsiouris3Ilias Tirodimos4Nikolaos Soultos5Anna Papa6Vangelis Economou7Laboratory of Animal Food Products Hygiene and Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Animal Food Products Hygiene and Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceUnit of Avian Medicine, Clinic of Farm Animals, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54627 Thessaloniki, GreeceUnit of Avian Medicine, Clinic of Farm Animals, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54627 Thessaloniki, GreeceLaboratory of Hygiene, Social & Preventive Medicine and Medical Statistics, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Animal Food Products Hygiene and Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Microbiology, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Animal Food Products Hygiene and Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceExtended-spectrum-β-lactamase (ESBL) and carbapenemase-producing <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i> and <i>Acinetobacter</i> spp. are associated with hospital-acquired infections and are commonly isolated across the poultry food production chain. Comprehensive data regarding the prevalence, spatiotemporal variations, and characterization of β-lactam-resistant bacteria in poultry farms and slaughterhouses is scarce. This study examines the prevalence and characteristics of β-lactam-resistant <i>E. coli</i>, <i>K. pneumoniae</i>, and <i>Acinetobacter</i> spp. isolated from poultry farms, slaughterhouses, and associated personnel in Greece. Strains were selectively isolated and identified via MALDI-TOF MS, which was also employed to identify possible relatedness. <i>E. coli</i> isolates were further classified into phylogenetic groups. The prevalence of β-lactam-resistant strains in farm and slaughterhouse environments was 15.0% (n = 15 strains)/57.3% (n = 71 strains) for <i>E. coli</i>, 11.0% (n = 11 strains)/1.6% (n = 2 strains) for <i>K. pneumoniae</i>, and 1.0% (n = 1 strain)/25.8% (n = 38 strains) for <i>Acinetobacter</i> spp., respectively. The prevalence of <i>Acinetobacter</i> spp. and <i>E. coli</i> on farmers’ skin was 16.7% (n = 2 strains) and 8.3% (n = 1 strain), correspondingly. Significantly higher <i>E. coli</i> isolation rates were observed in warmer seasons. All strains were multidrug-resistant and most carried ESBL/AmpC genes. Most <i>E. coli</i> isolates belonged to phylogroups A (41.4%, n = 36) and B1 (24.1%, n = 21). Proteomic analysis indicated relatedness among strains from different regions and seasons. Thus, poultry farms and slaughterhouses may serve as significant reservoirs of β-lactam-resistant strains of <i>E. coli</i>, <i>K. pneumoniae</i>, and <i>Acinetobacter</i> spp.https://www.mdpi.com/2304-8158/14/2/224antibiotic resistanceESKAPEβ-lactamasemultidrug resistancepoultry production chainenvironment |
| spellingShingle | Anestis Tsitsos Alexandros Damianos Konstantinos Kiskinis Vasilios Tsiouris Ilias Tirodimos Nikolaos Soultos Anna Papa Vangelis Economou Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece Foods antibiotic resistance ESKAPE β-lactamase multidrug resistance poultry production chain environment |
| title | Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece |
| title_full | Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece |
| title_fullStr | Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece |
| title_full_unstemmed | Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece |
| title_short | Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece |
| title_sort | prevalence characterization and proteomic relatedness among β lactam resistant bacteria throughout the poultry production chain in greece |
| topic | antibiotic resistance ESKAPE β-lactamase multidrug resistance poultry production chain environment |
| url | https://www.mdpi.com/2304-8158/14/2/224 |
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