Modulation of Biomaterial‐Associated Fibrosis by Means of Combined Physicochemical Material Properties
Abstract Biomaterial‐associated fibrosis remains a significant challenge in medical implants. To optimize implant design, understanding the interplay between biomaterials and host cells during the foreign body response (FBR) is crucial. Material properties are known to influence cellular behavior an...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202407531 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832582765461110784 |
|---|---|
| author | Lisa E. Tromp Torben A.B. van derBoon Roderick H.J. deHilster Ruud Bank Patrick van Rijn |
| author_facet | Lisa E. Tromp Torben A.B. van derBoon Roderick H.J. deHilster Ruud Bank Patrick van Rijn |
| author_sort | Lisa E. Tromp |
| collection | DOAJ |
| description | Abstract Biomaterial‐associated fibrosis remains a significant challenge in medical implants. To optimize implant design, understanding the interplay between biomaterials and host cells during the foreign body response (FBR) is crucial. Material properties are known to influence cellular behavior and can be used to manipulate cell responses, but predicting the right combination for the desired outcomes is challenging. This study explores how combined physicochemical material properties impact early myofibroblast differentiation using the Biomaterial Advanced Cell Screening (BiomACS) technology, which assesses hundreds of combinations of surface topography, stiffness, and wettability in a single experiment. Normal human dermal fibroblasts (NHDFs) are screened for cell density, area, and myofibroblast markers α‐smooth muscle actin (α‐SMA) and Collagen type I (COL1) after 24 h and 7 days of culture, with or without transforming growth factor‐beta (TGF‐β). Results demonstrated that material properties influence fibroblast behavior after 7 days with TGF‐β stimulation, with wettability emerging as the predominant factor, followed by stiffness. The study identified regions with increased cell adhesion while minimizing myofibroblast differentiation, offering the potential for implant surface optimization to prevent fibrosis. This research provides a powerful tool for cell‐material studies and represents a critical step toward enhancing implant properties and reducing complications, ultimately improving patient outcomes. |
| format | Article |
| id | doaj-art-9d90adde9b0c4d99a83ebf1a0a88f0ef |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-9d90adde9b0c4d99a83ebf1a0a88f0ef2025-01-29T09:50:19ZengWileyAdvanced Science2198-38442025-01-01124n/an/a10.1002/advs.202407531Modulation of Biomaterial‐Associated Fibrosis by Means of Combined Physicochemical Material PropertiesLisa E. Tromp0Torben A.B. van derBoon1Roderick H.J. deHilster2Ruud Bank3Patrick van Rijn4Department of Biomaterials and Biomedical Technology University of Groningen, University Medical Center Groningen FB‐40, A. Deusinglaan 1 Groningen 9713 AV the NetherlandsDepartment of Biomaterials and Biomedical Technology University of Groningen, University Medical Center Groningen FB‐40, A. Deusinglaan 1 Groningen 9713 AV the NetherlandsDepartment of Biomaterials and Biomedical Technology University of Groningen, University Medical Center Groningen FB‐40, A. Deusinglaan 1 Groningen 9713 AV the NetherlandsDepartment of Pathology and Medical Biology University of Groningen, University Medical Center Groningen A. Deusinglaan 1 Groningen 9713 AV the NetherlandsDepartment of Biomaterials and Biomedical Technology University of Groningen, University Medical Center Groningen FB‐40, A. Deusinglaan 1 Groningen 9713 AV the NetherlandsAbstract Biomaterial‐associated fibrosis remains a significant challenge in medical implants. To optimize implant design, understanding the interplay between biomaterials and host cells during the foreign body response (FBR) is crucial. Material properties are known to influence cellular behavior and can be used to manipulate cell responses, but predicting the right combination for the desired outcomes is challenging. This study explores how combined physicochemical material properties impact early myofibroblast differentiation using the Biomaterial Advanced Cell Screening (BiomACS) technology, which assesses hundreds of combinations of surface topography, stiffness, and wettability in a single experiment. Normal human dermal fibroblasts (NHDFs) are screened for cell density, area, and myofibroblast markers α‐smooth muscle actin (α‐SMA) and Collagen type I (COL1) after 24 h and 7 days of culture, with or without transforming growth factor‐beta (TGF‐β). Results demonstrated that material properties influence fibroblast behavior after 7 days with TGF‐β stimulation, with wettability emerging as the predominant factor, followed by stiffness. The study identified regions with increased cell adhesion while minimizing myofibroblast differentiation, offering the potential for implant surface optimization to prevent fibrosis. This research provides a powerful tool for cell‐material studies and represents a critical step toward enhancing implant properties and reducing complications, ultimately improving patient outcomes.https://doi.org/10.1002/advs.202407531biomaterial‐associated fibrosiscell‐material interactionsfibroblastforeign body responsehigh‐throughput screening |
| spellingShingle | Lisa E. Tromp Torben A.B. van derBoon Roderick H.J. deHilster Ruud Bank Patrick van Rijn Modulation of Biomaterial‐Associated Fibrosis by Means of Combined Physicochemical Material Properties Advanced Science biomaterial‐associated fibrosis cell‐material interactions fibroblast foreign body response high‐throughput screening |
| title | Modulation of Biomaterial‐Associated Fibrosis by Means of Combined Physicochemical Material Properties |
| title_full | Modulation of Biomaterial‐Associated Fibrosis by Means of Combined Physicochemical Material Properties |
| title_fullStr | Modulation of Biomaterial‐Associated Fibrosis by Means of Combined Physicochemical Material Properties |
| title_full_unstemmed | Modulation of Biomaterial‐Associated Fibrosis by Means of Combined Physicochemical Material Properties |
| title_short | Modulation of Biomaterial‐Associated Fibrosis by Means of Combined Physicochemical Material Properties |
| title_sort | modulation of biomaterial associated fibrosis by means of combined physicochemical material properties |
| topic | biomaterial‐associated fibrosis cell‐material interactions fibroblast foreign body response high‐throughput screening |
| url | https://doi.org/10.1002/advs.202407531 |
| work_keys_str_mv | AT lisaetromp modulationofbiomaterialassociatedfibrosisbymeansofcombinedphysicochemicalmaterialproperties AT torbenabvanderboon modulationofbiomaterialassociatedfibrosisbymeansofcombinedphysicochemicalmaterialproperties AT roderickhjdehilster modulationofbiomaterialassociatedfibrosisbymeansofcombinedphysicochemicalmaterialproperties AT ruudbank modulationofbiomaterialassociatedfibrosisbymeansofcombinedphysicochemicalmaterialproperties AT patrickvanrijn modulationofbiomaterialassociatedfibrosisbymeansofcombinedphysicochemicalmaterialproperties |