Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil

Abstract This study explored a novel laccase production process utilizing perennial biomass and the aqueous phase from bio-oil. Experimental and techno-economic modeling were coupled to gain insight into and optimize the solid-state fermentation conditions for minimizing the laccase selling price. A...

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Main Authors: Elmin Rahic, Nicholas Cassady, Kurt Rosentrater
Format: Article
Language:English
Published: SpringerOpen 2025-07-01
Series:Bioresources and Bioprocessing
Online Access:https://doi.org/10.1186/s40643-025-00924-2
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author Elmin Rahic
Nicholas Cassady
Kurt Rosentrater
author_facet Elmin Rahic
Nicholas Cassady
Kurt Rosentrater
author_sort Elmin Rahic
collection DOAJ
description Abstract This study explored a novel laccase production process utilizing perennial biomass and the aqueous phase from bio-oil. Experimental and techno-economic modeling were coupled to gain insight into and optimize the solid-state fermentation conditions for minimizing the laccase selling price. At relatively small scales, processing only 230 Mg of prairie biomass per year, a 5-year return on investment required a minimum laccase selling price of $0.05/kU, assuming a 10% discount rate. The associated sensitivity analysis showed the minimum laccase selling price to be most sensitive to parameters affecting laccase output from the plant, which included the number of batches produced per year and laccase recovery assumptions. Limitations and future outlooks were provided, emphasizing the need for further process optimization to minimize fermentation time and downstream losses.
format Article
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institution Kabale University
issn 2197-4365
language English
publishDate 2025-07-01
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record_format Article
series Bioresources and Bioprocessing
spelling doaj-art-9d89c7007ffc4379929bad5a480852322025-08-20T04:01:42ZengSpringerOpenBioresources and Bioprocessing2197-43652025-07-0112111310.1186/s40643-025-00924-2Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oilElmin Rahic0Nicholas Cassady1Kurt Rosentrater2Bioeconomy Institute, Iowa State UniversityDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State UniversityDepartment of Agricultural and Biosystems Engineering, Iowa State UniversityAbstract This study explored a novel laccase production process utilizing perennial biomass and the aqueous phase from bio-oil. Experimental and techno-economic modeling were coupled to gain insight into and optimize the solid-state fermentation conditions for minimizing the laccase selling price. At relatively small scales, processing only 230 Mg of prairie biomass per year, a 5-year return on investment required a minimum laccase selling price of $0.05/kU, assuming a 10% discount rate. The associated sensitivity analysis showed the minimum laccase selling price to be most sensitive to parameters affecting laccase output from the plant, which included the number of batches produced per year and laccase recovery assumptions. Limitations and future outlooks were provided, emphasizing the need for further process optimization to minimize fermentation time and downstream losses.https://doi.org/10.1186/s40643-025-00924-2
spellingShingle Elmin Rahic
Nicholas Cassady
Kurt Rosentrater
Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil
Bioresources and Bioprocessing
title Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil
title_full Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil
title_fullStr Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil
title_full_unstemmed Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil
title_short Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil
title_sort techno economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio oil
url https://doi.org/10.1186/s40643-025-00924-2
work_keys_str_mv AT elminrahic technoeconomicanalysisofanovellaccaseproductionprocessutilizingperennialbiomassandtheaqueousphaseofbiooil
AT nicholascassady technoeconomicanalysisofanovellaccaseproductionprocessutilizingperennialbiomassandtheaqueousphaseofbiooil
AT kurtrosentrater technoeconomicanalysisofanovellaccaseproductionprocessutilizingperennialbiomassandtheaqueousphaseofbiooil