Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound
Advanced glycosylation end products (AGEs) accumulate in diabetic wounds. Interactions between AGEs and their receptor (RAGE) leads to dermatologic problems in diabetes. Macrophage, which plays important roles in wound healing, highly expresses RAGE. Therefore, we investigated whether RAGE-expressin...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/1428537 |
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| _version_ | 1850170474569400320 |
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| author | Qi Wang Guanya Zhu Xiaozan Cao Jiaoyun Dong Fei Song Yiwen Niu |
| author_facet | Qi Wang Guanya Zhu Xiaozan Cao Jiaoyun Dong Fei Song Yiwen Niu |
| author_sort | Qi Wang |
| collection | DOAJ |
| description | Advanced glycosylation end products (AGEs) accumulate in diabetic wounds. Interactions between AGEs and their receptor (RAGE) leads to dermatologic problems in diabetes. Macrophage, which plays important roles in wound healing, highly expresses RAGE. Therefore, we investigated whether RAGE-expressing macrophages might be responsible for impaired wound healing on diabetes. We used anti-RAGE antibody applied topically on diabetic wounds. After confirming that wound healing was improved in anti-RAGE antibody group compared with normal mice, our results showed that macrophages appeared insufficient in the early stage and fading away slowly in the later proliferative phase compared with the control group, which was ameliorated in anti-RAGE antibody-applied wounds. Blocking AGE-RAGE signaling also increased neutrophils phagocytized by macrophages and promoted the phenotypic switch of macrophages from proinflammatory to prohealing activities. In vitro, phagocytosis of THP-1 (M0) and lipopolysaccharide- (LPS-) induced (M1) macrophages was impaired by treatment with AGEs, while IL-4- and IL-13-induced (M2) macrophages was not. Finally, AGEs increased the proinflammatory response of M1 macrophages, while inhibiting the polarization and anti-inflammatory functions of M2 macrophages. In conclusion, inhibition of AGE-RAGE signaling improved functional disorders of macrophages in the early inflammatory phase, which promoted the healing of wounds in diabetic mice. |
| format | Article |
| id | doaj-art-9d871f2e0d6c4720b8ee07e53edff446 |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-9d871f2e0d6c4720b8ee07e53edff4462025-08-20T02:20:29ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/14285371428537Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic WoundQi Wang0Guanya Zhu1Xiaozan Cao2Jiaoyun Dong3Fei Song4Yiwen Niu5Shanghai Burn Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Burn Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Burn Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Burn Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Burn Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Burn Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaAdvanced glycosylation end products (AGEs) accumulate in diabetic wounds. Interactions between AGEs and their receptor (RAGE) leads to dermatologic problems in diabetes. Macrophage, which plays important roles in wound healing, highly expresses RAGE. Therefore, we investigated whether RAGE-expressing macrophages might be responsible for impaired wound healing on diabetes. We used anti-RAGE antibody applied topically on diabetic wounds. After confirming that wound healing was improved in anti-RAGE antibody group compared with normal mice, our results showed that macrophages appeared insufficient in the early stage and fading away slowly in the later proliferative phase compared with the control group, which was ameliorated in anti-RAGE antibody-applied wounds. Blocking AGE-RAGE signaling also increased neutrophils phagocytized by macrophages and promoted the phenotypic switch of macrophages from proinflammatory to prohealing activities. In vitro, phagocytosis of THP-1 (M0) and lipopolysaccharide- (LPS-) induced (M1) macrophages was impaired by treatment with AGEs, while IL-4- and IL-13-induced (M2) macrophages was not. Finally, AGEs increased the proinflammatory response of M1 macrophages, while inhibiting the polarization and anti-inflammatory functions of M2 macrophages. In conclusion, inhibition of AGE-RAGE signaling improved functional disorders of macrophages in the early inflammatory phase, which promoted the healing of wounds in diabetic mice.http://dx.doi.org/10.1155/2017/1428537 |
| spellingShingle | Qi Wang Guanya Zhu Xiaozan Cao Jiaoyun Dong Fei Song Yiwen Niu Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound Journal of Diabetes Research |
| title | Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound |
| title_full | Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound |
| title_fullStr | Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound |
| title_full_unstemmed | Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound |
| title_short | Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound |
| title_sort | blocking age rage signaling improved functional disorders of macrophages in diabetic wound |
| url | http://dx.doi.org/10.1155/2017/1428537 |
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