Probiogenomic analysis of Limosilactobacillus fermentum SD7, a probiotic candidate with remarkable aggregation abilities
Limosilactobacillus fermentum has gained recognition as a probiotic due to its immunomodulatory properties. In this study, we characterized L. fermentum SD7, which was isolated from the human oral cavity. The genome of L. fermentum SD7 was approximately 2.27 Mb in size, with a 51.1 % GC content. Usi...
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Elsevier
2025-02-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402500831X |
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| author | Thunchanok Yaikhan Monwadee Wonglapsuwan Nuntiya Pahumunto Natakorn Nokchan Rawee Teanpaisan Komwit Surachat |
| author_facet | Thunchanok Yaikhan Monwadee Wonglapsuwan Nuntiya Pahumunto Natakorn Nokchan Rawee Teanpaisan Komwit Surachat |
| author_sort | Thunchanok Yaikhan |
| collection | DOAJ |
| description | Limosilactobacillus fermentum has gained recognition as a probiotic due to its immunomodulatory properties. In this study, we characterized L. fermentum SD7, which was isolated from the human oral cavity. The genome of L. fermentum SD7 was approximately 2.27 Mb in size, with a 51.1 % GC content. Using comprehensive genome analysis, we compared the genome of L. fermentum SD7 with 153 available genome sequences of L. fermentum strains and categorized the 154 strains into six distinct clades based on core gene single nucleotide polymorphisms. Among the 12,598 orthologous proteins, we identified 910 core genes and 10,169 accessory genes. Our analysis revealed a close similarity between L. fermentum SD7, FS-10, and L13. In addition, L. fermentum SD7 genome contains four strain-specific putative CRISPR-associated genes and lacks antimicrobial resistance and virulence genes. Importantly, we identified 27 genes in L. fermentum SD7 genome that are linked to aggregation ability, which is supported by our probiogenomic analysis. This aggregation ability is considered crucial for the probiotic efficacy of L. fermentum SD7. These findings provide a comprehensive understanding of the genetic composition of L. fermentum and its potential probiotic properties, identifying L. fermentum SD7 as a promising probiotic candidate for use in the food and healthcare industries. |
| format | Article |
| id | doaj-art-9d8230288d954f718cb1d4701dd64b50 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-9d8230288d954f718cb1d4701dd64b502025-02-09T05:00:41ZengElsevierHeliyon2405-84402025-02-01113e42451Probiogenomic analysis of Limosilactobacillus fermentum SD7, a probiotic candidate with remarkable aggregation abilitiesThunchanok Yaikhan0Monwadee Wonglapsuwan1Nuntiya Pahumunto2Natakorn Nokchan3Rawee Teanpaisan4Komwit Surachat5Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, ThailandDivision of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, ThailandResearch Center of Excellence for Oral Health, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, Thailand; Department of Oral Diagnostic Sciences, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, ThailandResearch Center of Excellence for Oral Health, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, Thailand; Medical Science Research and Innovation Institute, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Corresponding author. Medical Science Research and Innovation Institute, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Translational Medicine Research Center, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Corresponding author. Translational Medicine Research Center, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.Limosilactobacillus fermentum has gained recognition as a probiotic due to its immunomodulatory properties. In this study, we characterized L. fermentum SD7, which was isolated from the human oral cavity. The genome of L. fermentum SD7 was approximately 2.27 Mb in size, with a 51.1 % GC content. Using comprehensive genome analysis, we compared the genome of L. fermentum SD7 with 153 available genome sequences of L. fermentum strains and categorized the 154 strains into six distinct clades based on core gene single nucleotide polymorphisms. Among the 12,598 orthologous proteins, we identified 910 core genes and 10,169 accessory genes. Our analysis revealed a close similarity between L. fermentum SD7, FS-10, and L13. In addition, L. fermentum SD7 genome contains four strain-specific putative CRISPR-associated genes and lacks antimicrobial resistance and virulence genes. Importantly, we identified 27 genes in L. fermentum SD7 genome that are linked to aggregation ability, which is supported by our probiogenomic analysis. This aggregation ability is considered crucial for the probiotic efficacy of L. fermentum SD7. These findings provide a comprehensive understanding of the genetic composition of L. fermentum and its potential probiotic properties, identifying L. fermentum SD7 as a promising probiotic candidate for use in the food and healthcare industries.http://www.sciencedirect.com/science/article/pii/S240584402500831XLimosilactobacillus fermentumProbioticImmunomodulatoryWhole genome analysisAggregation ability |
| spellingShingle | Thunchanok Yaikhan Monwadee Wonglapsuwan Nuntiya Pahumunto Natakorn Nokchan Rawee Teanpaisan Komwit Surachat Probiogenomic analysis of Limosilactobacillus fermentum SD7, a probiotic candidate with remarkable aggregation abilities Heliyon Limosilactobacillus fermentum Probiotic Immunomodulatory Whole genome analysis Aggregation ability |
| title | Probiogenomic analysis of Limosilactobacillus fermentum SD7, a probiotic candidate with remarkable aggregation abilities |
| title_full | Probiogenomic analysis of Limosilactobacillus fermentum SD7, a probiotic candidate with remarkable aggregation abilities |
| title_fullStr | Probiogenomic analysis of Limosilactobacillus fermentum SD7, a probiotic candidate with remarkable aggregation abilities |
| title_full_unstemmed | Probiogenomic analysis of Limosilactobacillus fermentum SD7, a probiotic candidate with remarkable aggregation abilities |
| title_short | Probiogenomic analysis of Limosilactobacillus fermentum SD7, a probiotic candidate with remarkable aggregation abilities |
| title_sort | probiogenomic analysis of limosilactobacillus fermentum sd7 a probiotic candidate with remarkable aggregation abilities |
| topic | Limosilactobacillus fermentum Probiotic Immunomodulatory Whole genome analysis Aggregation ability |
| url | http://www.sciencedirect.com/science/article/pii/S240584402500831X |
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