Protocol to isolate glycoproteins from cultured mammalian cells and tissue samples using wheat germ agglutinin precipitation
Summary: Wheat germ agglutinin (WGA) precipitation enables selective enrichment of glycoproteins such as density-enhanced phosphatase (DEP-1) from protein lysates. Here, we present a protocol to isolate glycoproteins from cultured mammalian cells and tissue samples using WGA agarose beads. We descri...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003600 |
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