Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves’ Ophthalmopathy
Purpose. To evaluate the relationship between the expression of orbital tissue mRNA for FOXP3, CTLA-4/CD28/CD80/CD86, and CD40/CD40 and the severity of Graves’ orbitopathy (GO). Material and Methods. Orbital tissue was obtained from 26 patients with GO, with mild (n=6) or severe GO (n=20), and 7 hea...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/340934 |
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| author | Przemyslaw Pawlowski Natalia Wawrusiewicz-Kurylonek Anja Eckstein Joanna Reszec Wlodzimierz Luczynski Kristian Johnson Adam Kretowski Alina Bakunowicz-Lazarczyk Maria Gorska Jacek Szamatowicz Lech Chyczewski Janusz Mysliwiec |
| author_facet | Przemyslaw Pawlowski Natalia Wawrusiewicz-Kurylonek Anja Eckstein Joanna Reszec Wlodzimierz Luczynski Kristian Johnson Adam Kretowski Alina Bakunowicz-Lazarczyk Maria Gorska Jacek Szamatowicz Lech Chyczewski Janusz Mysliwiec |
| author_sort | Przemyslaw Pawlowski |
| collection | DOAJ |
| description | Purpose. To evaluate the relationship between the expression of orbital tissue mRNA for FOXP3, CTLA-4/CD28/CD80/CD86, and CD40/CD40 and the severity of Graves’ orbitopathy (GO). Material and Methods. Orbital tissue was obtained from 26 patients with GO, with mild (n=6) or severe GO (n=20), and 7 healthy controls. The expression of mRNA of FOXP3, CTLA-4/CD28/CD80/CD86, CD40/CD40L was measured by RT-PCR. TCR and CD3 were evaluated by immunohistochemistry. Results. Higher mRNA for FoxP3 (relative expression: 1.4) and CD40 (1.27) and lower expression of CTLA-4 (0.61) were found in the GO tissues versus controls. In severe GO as compared to mild GO higher mRNA expression for FoxP3 (1.35) and CD40 (1.4) and lower expression for CTLA-4 (0.78), CD28 (0.62), and CD40L (0.56) were found. A positive correlation was found between FOXP3 mRNA and CD3 infiltration (R=0.796, P=0.0000001). Conclusions. The enhanced FOXP3 mRNA expression in GO samples may suggest the dysfunction of FOXP3 cells in the severe GO. The diminished mRNA expression of CTLA-4 in severe GO may indicate inadequate T regulatory function. The enhanced mRNA expression of CD40 in severe GO and negative correlation to CRP mRNA may suggest their role in the active and inactive GO. |
| format | Article |
| id | doaj-art-9d8022c079b2406b881c1e175d478ba5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-9d8022c079b2406b881c1e175d478ba52025-02-03T05:46:01ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/340934340934Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves’ OphthalmopathyPrzemyslaw Pawlowski0Natalia Wawrusiewicz-Kurylonek1Anja Eckstein2Joanna Reszec3Wlodzimierz Luczynski4Kristian Johnson5Adam Kretowski6Alina Bakunowicz-Lazarczyk7Maria Gorska8Jacek Szamatowicz9Lech Chyczewski10Janusz Mysliwiec11Department of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Białystok, 13 Waszyngtona Street, 15-269 Białystok, PolandDepartment of Diabetology, Endocrinology and Internal Medicine, Medical University of Białystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, PolandDepartment of Ophthalmology, Essen University Hospital, University of Duisburg-Essen, Hufeland Straße 55, 45 122 Essen, GermanyDepartment of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Białystok, 13 Waszyngtona Street, 15-269 Białystok, PolandDepartment of Pediatrics, Endocrinology and Diabetology with Cardiology Division, Medical University of Bialystok, 17 Waszyngtona Street, 15-274 Białystok, PolandBayer HealthCare, 10 Kaiser-Wilhelm-Allee, 51373 Leverkusen, GermanyDepartment of Diabetology, Endocrinology and Internal Medicine, Medical University of Białystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, PolandDepartment of Pediatric Ophthalmology with Strabismus Treatment Unit, Medical University of Białystok, 17 Waszyngtona Street, 15-274 Białystok, PolandDepartment of Diabetology, Endocrinology and Internal Medicine, Medical University of Białystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, PolandDepartment of Gynaecology and Oncologic Gynaecology, Medical University of Bialystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, PolandDepartment of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Białystok, 13 Waszyngtona Street, 15-269 Białystok, PolandDepartment of Nuclear Medicine, Medical University of Bialystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, PolandPurpose. To evaluate the relationship between the expression of orbital tissue mRNA for FOXP3, CTLA-4/CD28/CD80/CD86, and CD40/CD40 and the severity of Graves’ orbitopathy (GO). Material and Methods. Orbital tissue was obtained from 26 patients with GO, with mild (n=6) or severe GO (n=20), and 7 healthy controls. The expression of mRNA of FOXP3, CTLA-4/CD28/CD80/CD86, CD40/CD40L was measured by RT-PCR. TCR and CD3 were evaluated by immunohistochemistry. Results. Higher mRNA for FoxP3 (relative expression: 1.4) and CD40 (1.27) and lower expression of CTLA-4 (0.61) were found in the GO tissues versus controls. In severe GO as compared to mild GO higher mRNA expression for FoxP3 (1.35) and CD40 (1.4) and lower expression for CTLA-4 (0.78), CD28 (0.62), and CD40L (0.56) were found. A positive correlation was found between FOXP3 mRNA and CD3 infiltration (R=0.796, P=0.0000001). Conclusions. The enhanced FOXP3 mRNA expression in GO samples may suggest the dysfunction of FOXP3 cells in the severe GO. The diminished mRNA expression of CTLA-4 in severe GO may indicate inadequate T regulatory function. The enhanced mRNA expression of CD40 in severe GO and negative correlation to CRP mRNA may suggest their role in the active and inactive GO.http://dx.doi.org/10.1155/2015/340934 |
| spellingShingle | Przemyslaw Pawlowski Natalia Wawrusiewicz-Kurylonek Anja Eckstein Joanna Reszec Wlodzimierz Luczynski Kristian Johnson Adam Kretowski Alina Bakunowicz-Lazarczyk Maria Gorska Jacek Szamatowicz Lech Chyczewski Janusz Mysliwiec Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves’ Ophthalmopathy Mediators of Inflammation |
| title | Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves’ Ophthalmopathy |
| title_full | Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves’ Ophthalmopathy |
| title_fullStr | Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves’ Ophthalmopathy |
| title_full_unstemmed | Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves’ Ophthalmopathy |
| title_short | Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves’ Ophthalmopathy |
| title_sort | disturbances of modulating molecules foxp3 ctla 4 cd28 b7 and cd40 cd40l mrna expressions in the orbital tissue from patients with severe graves ophthalmopathy |
| url | http://dx.doi.org/10.1155/2015/340934 |
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