Antibiotic resistance gene occurrence in poultry farms in northeast Brazil
The misuse of antibiotics in food-producing animal farming practices exerts selective pressure on bacterial strains, intensifying the spread of pathogenic and commensal bacteria carrying antibiotic resistance genes (ARGs). We conducted a study aiming to investigate ARGs in chicken litter from farms...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2025-02-01
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| Series: | Ciência Animal Brasileira |
| Online Access: | https://revistas.ufg.br/vet/article/view/79298 |
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| author | Henrique Francisco de Almeida Paulo Ricardo Conceição Marques Trindade César Roberto Viana Teixeira Claudson Oliveira Brito Silvio Santana Dolabella Sona Jain Maíra Pompeu Martins Ana Andréa Barbosa |
| author_facet | Henrique Francisco de Almeida Paulo Ricardo Conceição Marques Trindade César Roberto Viana Teixeira Claudson Oliveira Brito Silvio Santana Dolabella Sona Jain Maíra Pompeu Martins Ana Andréa Barbosa |
| author_sort | Henrique Francisco de Almeida |
| collection | DOAJ |
| description |
The misuse of antibiotics in food-producing animal farming practices exerts selective
pressure on bacterial strains, intensifying the spread of pathogenic and commensal bacteria carrying
antibiotic resistance genes (ARGs). We conducted a study aiming to investigate ARGs in chicken litter
from farms in the State of Sergipe, Northeast Brazil. A total of 14 chicken litter samples were collected
from twelve farms and subjected to total DNA extraction. The presence of ARGs in the obtained
material was tested by Polymerase Chain Reaction (PCR) using primers for selected ARGs. ARGs
were confirmed in all samples, and the highest resistance positivity was obtained for tetracyclines
(tetA, tetM, and tetG), quinolones (gyrA and qnrS), beta-lactams (blaTEM), macrolides (ermB) and
sulfonamides (sul-1). Sequencing and comparison with the GenBank database confirmed the identity
of the ARGs. Some of the sequences that were amplified by PCR were similar to resistance factors
found in Gram-positive and Gram-negative bacteria of different species, mostly enterobacteria.
Furthermore, similarity was observed for resistance determinants located both on the chromosome
and on plasmids, transposons, and integrons. Our results indicate the potential of poultry farming
for the environmental dissemination of ARGs in the State of Sergipe.
|
| format | Article |
| id | doaj-art-9d7d25d415ee47e7bc52a7fbe6bfe77d |
| institution | Kabale University |
| issn | 1518-2797 1809-6891 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj-art-9d7d25d415ee47e7bc52a7fbe6bfe77d2025-02-08T20:24:08ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912025-02-0126Antibiotic resistance gene occurrence in poultry farms in northeast BrazilHenrique Francisco de Almeida0Paulo Ricardo Conceição Marques Trindade1César Roberto Viana Teixeira2Claudson Oliveira Brito3Silvio Santana Dolabella4Sona Jain5Maíra Pompeu Martins6Ana Andréa Barbosa7Universidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, BrasilUniversidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, BrasilUniversidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, BrasilUniversidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, BrasilUniversidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, BrasilUniversidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, BrasilUniversidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, BrasilUniversidade Federal de Sergipe (UFS), São Cristóvão, Sergipe, Brasil The misuse of antibiotics in food-producing animal farming practices exerts selective pressure on bacterial strains, intensifying the spread of pathogenic and commensal bacteria carrying antibiotic resistance genes (ARGs). We conducted a study aiming to investigate ARGs in chicken litter from farms in the State of Sergipe, Northeast Brazil. A total of 14 chicken litter samples were collected from twelve farms and subjected to total DNA extraction. The presence of ARGs in the obtained material was tested by Polymerase Chain Reaction (PCR) using primers for selected ARGs. ARGs were confirmed in all samples, and the highest resistance positivity was obtained for tetracyclines (tetA, tetM, and tetG), quinolones (gyrA and qnrS), beta-lactams (blaTEM), macrolides (ermB) and sulfonamides (sul-1). Sequencing and comparison with the GenBank database confirmed the identity of the ARGs. Some of the sequences that were amplified by PCR were similar to resistance factors found in Gram-positive and Gram-negative bacteria of different species, mostly enterobacteria. Furthermore, similarity was observed for resistance determinants located both on the chromosome and on plasmids, transposons, and integrons. Our results indicate the potential of poultry farming for the environmental dissemination of ARGs in the State of Sergipe. https://revistas.ufg.br/vet/article/view/79298 |
| spellingShingle | Henrique Francisco de Almeida Paulo Ricardo Conceição Marques Trindade César Roberto Viana Teixeira Claudson Oliveira Brito Silvio Santana Dolabella Sona Jain Maíra Pompeu Martins Ana Andréa Barbosa Antibiotic resistance gene occurrence in poultry farms in northeast Brazil Ciência Animal Brasileira |
| title | Antibiotic resistance gene occurrence in poultry farms in northeast Brazil |
| title_full | Antibiotic resistance gene occurrence in poultry farms in northeast Brazil |
| title_fullStr | Antibiotic resistance gene occurrence in poultry farms in northeast Brazil |
| title_full_unstemmed | Antibiotic resistance gene occurrence in poultry farms in northeast Brazil |
| title_short | Antibiotic resistance gene occurrence in poultry farms in northeast Brazil |
| title_sort | antibiotic resistance gene occurrence in poultry farms in northeast brazil |
| url | https://revistas.ufg.br/vet/article/view/79298 |
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