Functional and Therapeutic Relevance of Rho GTPases in Innate Immune Cell Migration and Function during Inflammation: An In Silico Perspective

Systematic regulation of leukocyte migration to the site of infection is a vital step during immunological responses. Improper migration and localization of immune cells could be associated with disease pathology as seen in systemic inflammation. Rho GTPases act as molecular switches during inflamma...

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Main Authors: Pankaj Dipankar, Puneet Kumar, Shiba Prasad Dash, Pranita P. Sarangi
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2021/6655412
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author Pankaj Dipankar
Puneet Kumar
Shiba Prasad Dash
Pranita P. Sarangi
author_facet Pankaj Dipankar
Puneet Kumar
Shiba Prasad Dash
Pranita P. Sarangi
author_sort Pankaj Dipankar
collection DOAJ
description Systematic regulation of leukocyte migration to the site of infection is a vital step during immunological responses. Improper migration and localization of immune cells could be associated with disease pathology as seen in systemic inflammation. Rho GTPases act as molecular switches during inflammatory cell migration by cycling between Rho-GDP (inactive) to Rho-GTP (active) forms and play an essential role in the precise regulation of actin cytoskeletal dynamics as well as other immunological functions of leukocytes. Available reports suggest that the dysregulation of Rho GTPase signaling is associated with various inflammatory diseases ranging from mild to life-threatening conditions. Therefore, it is crucial to understand the step-by-step activation and inactivation of GTPases and the functioning of different Guanine Nucleotide Exchange Factors (GEFs) and GTPase-Activating Proteins (GAPs) that regulate the conversion of GDP to GTP and GTP to GDP exchange reactions, respectively. Here, we describe the molecular organization and activation of various domains of crucial elements associated with the activation of Rho GTPases using solved PDB structures. We will also present the latest evidence available on the relevance of Rho GTPases in the migration and function of innate immune cells during inflammation. This knowledge will help scientists design promising drug candidates against the Rho-GTPase-centric regulatory molecules regulating inflammatory cell migration.
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spelling doaj-art-9d6d2d73ec194ec8afeea1e014c9166c2025-08-20T03:38:23ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/66554126655412Functional and Therapeutic Relevance of Rho GTPases in Innate Immune Cell Migration and Function during Inflammation: An In Silico PerspectivePankaj Dipankar0Puneet Kumar1Shiba Prasad Dash2Pranita P. Sarangi3Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, IndiaDepartment of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, IndiaDepartment of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, IndiaDepartment of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, IndiaSystematic regulation of leukocyte migration to the site of infection is a vital step during immunological responses. Improper migration and localization of immune cells could be associated with disease pathology as seen in systemic inflammation. Rho GTPases act as molecular switches during inflammatory cell migration by cycling between Rho-GDP (inactive) to Rho-GTP (active) forms and play an essential role in the precise regulation of actin cytoskeletal dynamics as well as other immunological functions of leukocytes. Available reports suggest that the dysregulation of Rho GTPase signaling is associated with various inflammatory diseases ranging from mild to life-threatening conditions. Therefore, it is crucial to understand the step-by-step activation and inactivation of GTPases and the functioning of different Guanine Nucleotide Exchange Factors (GEFs) and GTPase-Activating Proteins (GAPs) that regulate the conversion of GDP to GTP and GTP to GDP exchange reactions, respectively. Here, we describe the molecular organization and activation of various domains of crucial elements associated with the activation of Rho GTPases using solved PDB structures. We will also present the latest evidence available on the relevance of Rho GTPases in the migration and function of innate immune cells during inflammation. This knowledge will help scientists design promising drug candidates against the Rho-GTPase-centric regulatory molecules regulating inflammatory cell migration.http://dx.doi.org/10.1155/2021/6655412
spellingShingle Pankaj Dipankar
Puneet Kumar
Shiba Prasad Dash
Pranita P. Sarangi
Functional and Therapeutic Relevance of Rho GTPases in Innate Immune Cell Migration and Function during Inflammation: An In Silico Perspective
Mediators of Inflammation
title Functional and Therapeutic Relevance of Rho GTPases in Innate Immune Cell Migration and Function during Inflammation: An In Silico Perspective
title_full Functional and Therapeutic Relevance of Rho GTPases in Innate Immune Cell Migration and Function during Inflammation: An In Silico Perspective
title_fullStr Functional and Therapeutic Relevance of Rho GTPases in Innate Immune Cell Migration and Function during Inflammation: An In Silico Perspective
title_full_unstemmed Functional and Therapeutic Relevance of Rho GTPases in Innate Immune Cell Migration and Function during Inflammation: An In Silico Perspective
title_short Functional and Therapeutic Relevance of Rho GTPases in Innate Immune Cell Migration and Function during Inflammation: An In Silico Perspective
title_sort functional and therapeutic relevance of rho gtpases in innate immune cell migration and function during inflammation an in silico perspective
url http://dx.doi.org/10.1155/2021/6655412
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