New insights into ovarian regression-related mitochondrial dysfunction in the late-laying period
Duck egg production sharply decreases during the late-laying period, which likely stems from an ovarian mechanism. However, the molecular mechanisms underlying ovarian regression during the late-laying period remain unclear. In this study, egg-laying (LLP) and ceased-laying (CLP) ducks at 72 weeks o...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125001774 |
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| author | Tiantian Gu Jinyu Liu Tao Zeng Li Chen Yong Tian Wenwu Xu Lizhi Lu |
| author_facet | Tiantian Gu Jinyu Liu Tao Zeng Li Chen Yong Tian Wenwu Xu Lizhi Lu |
| author_sort | Tiantian Gu |
| collection | DOAJ |
| description | Duck egg production sharply decreases during the late-laying period, which likely stems from an ovarian mechanism. However, the molecular mechanisms underlying ovarian regression during the late-laying period remain unclear. In this study, egg-laying (LLP) and ceased-laying (CLP) ducks at 72 weeks of age were selected to explore the potential mechanism of ovarian regression. Proteomic analysis demonstrated the importance of mitochondrial function in ovarian regression. Notably, metabolomic analysis showed that CLP ducks disturbed TCA cycle, as exhibited by the lower fumarate content. The ovarian expression of protein markers for mitochondrial biogenesis (PGC-1α and TFAM) and function (SIRT1 and SIRT3) were suppressed in CLP ducks. CLP ducks had significantly increased MDA levels and reduced SOD, CAT, GSH-Px, and T-AOC activities, inducing excessive oxidative stress. Interestingly, ACSL4, a key regulator of ferroptosis, was associated with the mitochondrial envelope and membrane function during ovarian regression. CLP ducks showed significantly reduced GSH levels and increased Fe2+ content, as well as decreased the expression of ferroptosis-related proteins (GPX4 and SLC7A11) and antioxidant-related proteins (COX2, CAT, SOD1, and SOD2). Collectively, our findings suggest that ovarian regression-mediated mitochondrial dysfunction contributes to oxidative stress-induced ferroptosis in ducks that have ceased laying. |
| format | Article |
| id | doaj-art-9d678e822f0a4bb98286fedab0a40bd0 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-9d678e822f0a4bb98286fedab0a40bd02025-08-20T02:03:59ZengElsevierPoultry Science0032-57912025-04-01104410493810.1016/j.psj.2025.104938New insights into ovarian regression-related mitochondrial dysfunction in the late-laying periodTiantian Gu0Jinyu Liu1Tao Zeng2Li Chen3Yong Tian4Wenwu Xu5Lizhi Lu6Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaCorresponding author.; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaDuck egg production sharply decreases during the late-laying period, which likely stems from an ovarian mechanism. However, the molecular mechanisms underlying ovarian regression during the late-laying period remain unclear. In this study, egg-laying (LLP) and ceased-laying (CLP) ducks at 72 weeks of age were selected to explore the potential mechanism of ovarian regression. Proteomic analysis demonstrated the importance of mitochondrial function in ovarian regression. Notably, metabolomic analysis showed that CLP ducks disturbed TCA cycle, as exhibited by the lower fumarate content. The ovarian expression of protein markers for mitochondrial biogenesis (PGC-1α and TFAM) and function (SIRT1 and SIRT3) were suppressed in CLP ducks. CLP ducks had significantly increased MDA levels and reduced SOD, CAT, GSH-Px, and T-AOC activities, inducing excessive oxidative stress. Interestingly, ACSL4, a key regulator of ferroptosis, was associated with the mitochondrial envelope and membrane function during ovarian regression. CLP ducks showed significantly reduced GSH levels and increased Fe2+ content, as well as decreased the expression of ferroptosis-related proteins (GPX4 and SLC7A11) and antioxidant-related proteins (COX2, CAT, SOD1, and SOD2). Collectively, our findings suggest that ovarian regression-mediated mitochondrial dysfunction contributes to oxidative stress-induced ferroptosis in ducks that have ceased laying.http://www.sciencedirect.com/science/article/pii/S0032579125001774Ovarian regressionMitochondrial dysfunctionOxidative stressFerroptosisCeased-laying duck |
| spellingShingle | Tiantian Gu Jinyu Liu Tao Zeng Li Chen Yong Tian Wenwu Xu Lizhi Lu New insights into ovarian regression-related mitochondrial dysfunction in the late-laying period Poultry Science Ovarian regression Mitochondrial dysfunction Oxidative stress Ferroptosis Ceased-laying duck |
| title | New insights into ovarian regression-related mitochondrial dysfunction in the late-laying period |
| title_full | New insights into ovarian regression-related mitochondrial dysfunction in the late-laying period |
| title_fullStr | New insights into ovarian regression-related mitochondrial dysfunction in the late-laying period |
| title_full_unstemmed | New insights into ovarian regression-related mitochondrial dysfunction in the late-laying period |
| title_short | New insights into ovarian regression-related mitochondrial dysfunction in the late-laying period |
| title_sort | new insights into ovarian regression related mitochondrial dysfunction in the late laying period |
| topic | Ovarian regression Mitochondrial dysfunction Oxidative stress Ferroptosis Ceased-laying duck |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125001774 |
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