An inconvenient impact: Unveiling the overlooked differences in crystalline forms of iron (hydro)oxides on anaerobic digestion

Iron (hydro)oxides are commonly used to enhance anaerobic digestion due to their cost-effectiveness and versatility. However, the influence of crystalline structure on digestion performance is often overlooked despite their unique characteristics. In this study, we investigated how different crystal...

Full description

Saved in:
Bibliographic Details
Main Authors: Han-Quan Wen, Guan-Lin Chen, Yu-Sheng Li, Tian Tian, Yuan Pan, Han-Qing Yu
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Water Research X
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2589914724000768
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850037664619692032
author Han-Quan Wen
Guan-Lin Chen
Yu-Sheng Li
Tian Tian
Yuan Pan
Han-Qing Yu
author_facet Han-Quan Wen
Guan-Lin Chen
Yu-Sheng Li
Tian Tian
Yuan Pan
Han-Qing Yu
author_sort Han-Quan Wen
collection DOAJ
description Iron (hydro)oxides are commonly used to enhance anaerobic digestion due to their cost-effectiveness and versatility. However, the influence of crystalline structure on digestion performance is often overlooked despite their unique characteristics. In this study, we investigated how different crystalline forms of FeOOH affect substrate utilization, sludge activity, and the microbiomes in up-flow anaerobic sludge blanket (UASB) reactors. The crystalline structure of FeOOH impacted reactor performance, with γ-FeOOH, β-FeOOH, and α-FeOOH showing decreasing effectiveness, as reflected in chemical oxygen demand (COD) removal efficiencies of 99.0 %, 98.3 % and 97.1 %, respectively. FeOOH crystals influenced the secretion of extracellular polymeric substances (EPS) and sludge activity by releasing Fe ions at varying rates, leading to Fe accumulation in EPS in the order of β-FeOOH > γ-FeOOH > α-FeOOH. Additionally, γ-FeOOH supported the most stable microbial community structure, as indicated by the highest Alpha diversity index. This stability was associated with increased levels of Mesotoga and Syntrophus, along with the highest coenzyme F420 activity, which was approximately twice as high as in other groups. These findings underscore the crucial role of the crystalline structure of iron oxides in enhancing anaerobic digestion, emphasizing that biocompatibility should be a priority when optimizing digestion performance.
format Article
id doaj-art-9d5dd4f5c88d42eab5bdd6a107080889
institution DOAJ
issn 2589-9147
language English
publishDate 2025-01-01
publisher Elsevier
record_format Article
series Water Research X
spelling doaj-art-9d5dd4f5c88d42eab5bdd6a1070808892025-08-20T02:56:47ZengElsevierWater Research X2589-91472025-01-012610028610.1016/j.wroa.2024.100286An inconvenient impact: Unveiling the overlooked differences in crystalline forms of iron (hydro)oxides on anaerobic digestionHan-Quan Wen0Guan-Lin Chen1Yu-Sheng Li2Tian Tian3Yuan Pan4Han-Qing Yu5CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Technology, University of Science and Technology of China, Hefei 230026, ChinaCAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Technology, University of Science and Technology of China, Hefei 230026, ChinaCAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Technology, University of Science and Technology of China, Hefei 230026, China; Institute of Advanced Technology, University of Science and Technology of China, Hefei 230000, ChinaKey Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China; Corresponding authors.CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Technology, University of Science and Technology of China, Hefei 230026, ChinaCAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Technology, University of Science and Technology of China, Hefei 230026, China; Corresponding authors.Iron (hydro)oxides are commonly used to enhance anaerobic digestion due to their cost-effectiveness and versatility. However, the influence of crystalline structure on digestion performance is often overlooked despite their unique characteristics. In this study, we investigated how different crystalline forms of FeOOH affect substrate utilization, sludge activity, and the microbiomes in up-flow anaerobic sludge blanket (UASB) reactors. The crystalline structure of FeOOH impacted reactor performance, with γ-FeOOH, β-FeOOH, and α-FeOOH showing decreasing effectiveness, as reflected in chemical oxygen demand (COD) removal efficiencies of 99.0 %, 98.3 % and 97.1 %, respectively. FeOOH crystals influenced the secretion of extracellular polymeric substances (EPS) and sludge activity by releasing Fe ions at varying rates, leading to Fe accumulation in EPS in the order of β-FeOOH > γ-FeOOH > α-FeOOH. Additionally, γ-FeOOH supported the most stable microbial community structure, as indicated by the highest Alpha diversity index. This stability was associated with increased levels of Mesotoga and Syntrophus, along with the highest coenzyme F420 activity, which was approximately twice as high as in other groups. These findings underscore the crucial role of the crystalline structure of iron oxides in enhancing anaerobic digestion, emphasizing that biocompatibility should be a priority when optimizing digestion performance.http://www.sciencedirect.com/science/article/pii/S2589914724000768Anaerobic digestionFeOOHcrystalline formEPSMetagenome
spellingShingle Han-Quan Wen
Guan-Lin Chen
Yu-Sheng Li
Tian Tian
Yuan Pan
Han-Qing Yu
An inconvenient impact: Unveiling the overlooked differences in crystalline forms of iron (hydro)oxides on anaerobic digestion
Water Research X
Anaerobic digestion
FeOOH
crystalline form
EPS
Metagenome
title An inconvenient impact: Unveiling the overlooked differences in crystalline forms of iron (hydro)oxides on anaerobic digestion
title_full An inconvenient impact: Unveiling the overlooked differences in crystalline forms of iron (hydro)oxides on anaerobic digestion
title_fullStr An inconvenient impact: Unveiling the overlooked differences in crystalline forms of iron (hydro)oxides on anaerobic digestion
title_full_unstemmed An inconvenient impact: Unveiling the overlooked differences in crystalline forms of iron (hydro)oxides on anaerobic digestion
title_short An inconvenient impact: Unveiling the overlooked differences in crystalline forms of iron (hydro)oxides on anaerobic digestion
title_sort inconvenient impact unveiling the overlooked differences in crystalline forms of iron hydro oxides on anaerobic digestion
topic Anaerobic digestion
FeOOH
crystalline form
EPS
Metagenome
url http://www.sciencedirect.com/science/article/pii/S2589914724000768
work_keys_str_mv AT hanquanwen aninconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT guanlinchen aninconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT yushengli aninconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT tiantian aninconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT yuanpan aninconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT hanqingyu aninconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT hanquanwen inconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT guanlinchen inconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT yushengli inconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT tiantian inconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT yuanpan inconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion
AT hanqingyu inconvenientimpactunveilingtheoverlookeddifferencesincrystallineformsofironhydrooxidesonanaerobicdigestion