Laparoscopic lavage in a purulent peritonitis model: impact on inflammatory proteins
Abstract Background Laparoscopic lavage is an effective, safe, and feasible treatment in patients with perforated diverticulitis with purulent peritonitis. Laparoscopic lavage was introduced without any detailed knowledge regarding the mechanisms of action. The aim of this study was to validate the...
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BMC
2025-03-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-025-02445-2 |
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| author | Erik Sinclair Maria K. Magnusson Eva Angenete Mattias Prytz Viktor Tasselius Lena Öhman Eva Haglind |
| author_facet | Erik Sinclair Maria K. Magnusson Eva Angenete Mattias Prytz Viktor Tasselius Lena Öhman Eva Haglind |
| author_sort | Erik Sinclair |
| collection | DOAJ |
| description | Abstract Background Laparoscopic lavage is an effective, safe, and feasible treatment in patients with perforated diverticulitis with purulent peritonitis. Laparoscopic lavage was introduced without any detailed knowledge regarding the mechanisms of action. The aim of this study was to validate the reproducibility of an animal model of purulent peritonitis and to study the effect of laparoscopic lavage on inflammatory proteins in this model. Methods Forty rats, divided into eight groups (n = 5) were operated. Six groups underwent cecal ligation and puncture (CLP) causing peritonitis and two groups underwent sham surgery. Three CLP and one sham group received laparoscopic lavage, while the remaining groups acted as time-matched controls. Samples of abdominal fluid and blood were collected after 1, 2 or 3 h and analyzed regarding 92 inflammatory proteins using Olink Target 96 Mouse exploratory panel. Results Animals with peritonitis had higher levels of inflammatory proteins such as CCL3, IL17A and IL6 in abdominal fluid and serum compared to sham. The groups treated with laparoscopic lavage had lower levels of inflammatory proteins in both abdominal fluid and serum compared with untreated peritonitis groups, results were most distinct sampled after one hour. Conclusion Our animal model is reproducible, and mimics perforated diverticulitis with purulent peritonitis with increased levels of inflammatory proteins in abdominal fluid and serum. The levels of several inflammatory proteins were lower following laparoscopic lavage treatment perhaps indicating the physiological effect of laparoscopic lavage. This model can be used to further explore the mechanisms involved in peritonitis and laparoscopic lavage treatment. |
| format | Article |
| id | doaj-art-9d5ce04cdc0b4c1aa10276f84d0faa60 |
| institution | DOAJ |
| issn | 2047-783X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
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| series | European Journal of Medical Research |
| spelling | doaj-art-9d5ce04cdc0b4c1aa10276f84d0faa602025-08-20T02:51:23ZengBMCEuropean Journal of Medical Research2047-783X2025-03-0130111010.1186/s40001-025-02445-2Laparoscopic lavage in a purulent peritonitis model: impact on inflammatory proteinsErik Sinclair0Maria K. Magnusson1Eva Angenete2Mattias Prytz3Viktor Tasselius4Lena Öhman5Eva Haglind6Department of Surgery, Scandinavian Surgical Outcomes Research Group, Institute of Clinical Sciences, Sahlgrenska Academy, University of GothenburgDepartment of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of GothenburgDepartment of Surgery, Scandinavian Surgical Outcomes Research Group, Institute of Clinical Sciences, Sahlgrenska Academy, University of GothenburgDepartment of Surgery, Region Västra Götaland, NU-Hospital GroupSchool of Public Health and Community Medicine, Institute of Medicine, University of GothenburgDepartment of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of GothenburgDepartment of Surgery, Scandinavian Surgical Outcomes Research Group, Institute of Clinical Sciences, Sahlgrenska Academy, University of GothenburgAbstract Background Laparoscopic lavage is an effective, safe, and feasible treatment in patients with perforated diverticulitis with purulent peritonitis. Laparoscopic lavage was introduced without any detailed knowledge regarding the mechanisms of action. The aim of this study was to validate the reproducibility of an animal model of purulent peritonitis and to study the effect of laparoscopic lavage on inflammatory proteins in this model. Methods Forty rats, divided into eight groups (n = 5) were operated. Six groups underwent cecal ligation and puncture (CLP) causing peritonitis and two groups underwent sham surgery. Three CLP and one sham group received laparoscopic lavage, while the remaining groups acted as time-matched controls. Samples of abdominal fluid and blood were collected after 1, 2 or 3 h and analyzed regarding 92 inflammatory proteins using Olink Target 96 Mouse exploratory panel. Results Animals with peritonitis had higher levels of inflammatory proteins such as CCL3, IL17A and IL6 in abdominal fluid and serum compared to sham. The groups treated with laparoscopic lavage had lower levels of inflammatory proteins in both abdominal fluid and serum compared with untreated peritonitis groups, results were most distinct sampled after one hour. Conclusion Our animal model is reproducible, and mimics perforated diverticulitis with purulent peritonitis with increased levels of inflammatory proteins in abdominal fluid and serum. The levels of several inflammatory proteins were lower following laparoscopic lavage treatment perhaps indicating the physiological effect of laparoscopic lavage. This model can be used to further explore the mechanisms involved in peritonitis and laparoscopic lavage treatment.https://doi.org/10.1186/s40001-025-02445-2CLPHinchey grade III diverticulitisPeritonitisExperimentalLaparoscopic lavageInflammation |
| spellingShingle | Erik Sinclair Maria K. Magnusson Eva Angenete Mattias Prytz Viktor Tasselius Lena Öhman Eva Haglind Laparoscopic lavage in a purulent peritonitis model: impact on inflammatory proteins European Journal of Medical Research CLP Hinchey grade III diverticulitis Peritonitis Experimental Laparoscopic lavage Inflammation |
| title | Laparoscopic lavage in a purulent peritonitis model: impact on inflammatory proteins |
| title_full | Laparoscopic lavage in a purulent peritonitis model: impact on inflammatory proteins |
| title_fullStr | Laparoscopic lavage in a purulent peritonitis model: impact on inflammatory proteins |
| title_full_unstemmed | Laparoscopic lavage in a purulent peritonitis model: impact on inflammatory proteins |
| title_short | Laparoscopic lavage in a purulent peritonitis model: impact on inflammatory proteins |
| title_sort | laparoscopic lavage in a purulent peritonitis model impact on inflammatory proteins |
| topic | CLP Hinchey grade III diverticulitis Peritonitis Experimental Laparoscopic lavage Inflammation |
| url | https://doi.org/10.1186/s40001-025-02445-2 |
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