Inhibitory Effect of Exosomes Loaded with Ginsenoside Rh2 on Growth of Hepatocellular Carcinoma Cells
ObjectiveTo explore the effects of exosomes loaded with ginsenoside Rh2 on the biological functions of hepatocellular carcinoma cells. MethodsBoth Huh7 and PLC/PRF/5 cell were equally divided into control group, exosome group (Exos group), drug group (G-Rh2 group), and exosomes-loaded-with-ginsenosi...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Magazine House of Cancer Research on Prevention and Treatment
2025-07-01
|
| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.1326 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849304034382446592 |
|---|---|
| author | Meile LI Ziyue ZHAO Kai JIN Yu’an XIE |
| author_facet | Meile LI Ziyue ZHAO Kai JIN Yu’an XIE |
| author_sort | Meile LI |
| collection | DOAJ |
| description | ObjectiveTo explore the effects of exosomes loaded with ginsenoside Rh2 on the biological functions of hepatocellular carcinoma cells. MethodsBoth Huh7 and PLC/PRF/5 cell were equally divided into control group, exosome group (Exos group), drug group (G-Rh2 group), and exosomes-loaded-with-ginsenoside Rh2 group (Exos@G-Rh2 group). The effects of each group on the viability, clonogenic ability, migration ability, invasion ability, and apoptotic level of hepatocellular carcinoma cells were detected through CCK-8 assay, colony formation assay, cell scratch assay, Transwell assay, and flow cytometry. ResultsCompared with the control group, the Exos@G-Rh2 group and G-Rh2 group showed significantly decreased cell viability, clonogenic ability, and migration and invasion capabilities, along with a markedly increased cell apoptosis rate (P<0.05). These changes were more pronounced in the Exos@G-Rh2 group than in the G-Rh2 group (P<0.05). ConclusionExos@G-Rh2 can effectively inhibit the viability and clonogenic, migration, and invasion abilities of liver cancer cells and induce cell apoptosis. This effect is stronger than that of free G-Rh2 at the same concentration. |
| format | Article |
| id | doaj-art-9d559b976100485096e586a25797bf44 |
| institution | Kabale University |
| issn | 1000-8578 |
| language | zho |
| publishDate | 2025-07-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj-art-9d559b976100485096e586a25797bf442025-08-20T03:55:49ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782025-07-0152757858410.3971/j.issn.1000-8578.2025.24.132620241326Inhibitory Effect of Exosomes Loaded with Ginsenoside Rh2 on Growth of Hepatocellular Carcinoma CellsMeile LI0Ziyue ZHAO1Kai JIN2Yu’an XIE3Department of Oncology, Jiangbin Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaReproductive Health and Birth Defects Prevention and Control Key Laboratory of Guangxi Maternity and Child Health Care Hospital, Nanning 530021, ChinaReproductive Health and Birth Defects Prevention and Control Key Laboratory of Guangxi Maternity and Child Health Care Hospital, Nanning 530021, ChinaObjectiveTo explore the effects of exosomes loaded with ginsenoside Rh2 on the biological functions of hepatocellular carcinoma cells. MethodsBoth Huh7 and PLC/PRF/5 cell were equally divided into control group, exosome group (Exos group), drug group (G-Rh2 group), and exosomes-loaded-with-ginsenoside Rh2 group (Exos@G-Rh2 group). The effects of each group on the viability, clonogenic ability, migration ability, invasion ability, and apoptotic level of hepatocellular carcinoma cells were detected through CCK-8 assay, colony formation assay, cell scratch assay, Transwell assay, and flow cytometry. ResultsCompared with the control group, the Exos@G-Rh2 group and G-Rh2 group showed significantly decreased cell viability, clonogenic ability, and migration and invasion capabilities, along with a markedly increased cell apoptosis rate (P<0.05). These changes were more pronounced in the Exos@G-Rh2 group than in the G-Rh2 group (P<0.05). ConclusionExos@G-Rh2 can effectively inhibit the viability and clonogenic, migration, and invasion abilities of liver cancer cells and induce cell apoptosis. This effect is stronger than that of free G-Rh2 at the same concentration.http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.1326hepatocellular carcinomaginsenoside rh2exosomes |
| spellingShingle | Meile LI Ziyue ZHAO Kai JIN Yu’an XIE Inhibitory Effect of Exosomes Loaded with Ginsenoside Rh2 on Growth of Hepatocellular Carcinoma Cells Zhongliu Fangzhi Yanjiu hepatocellular carcinoma ginsenoside rh2 exosomes |
| title | Inhibitory Effect of Exosomes Loaded with Ginsenoside Rh2 on Growth of Hepatocellular Carcinoma Cells |
| title_full | Inhibitory Effect of Exosomes Loaded with Ginsenoside Rh2 on Growth of Hepatocellular Carcinoma Cells |
| title_fullStr | Inhibitory Effect of Exosomes Loaded with Ginsenoside Rh2 on Growth of Hepatocellular Carcinoma Cells |
| title_full_unstemmed | Inhibitory Effect of Exosomes Loaded with Ginsenoside Rh2 on Growth of Hepatocellular Carcinoma Cells |
| title_short | Inhibitory Effect of Exosomes Loaded with Ginsenoside Rh2 on Growth of Hepatocellular Carcinoma Cells |
| title_sort | inhibitory effect of exosomes loaded with ginsenoside rh2 on growth of hepatocellular carcinoma cells |
| topic | hepatocellular carcinoma ginsenoside rh2 exosomes |
| url | http://www.zlfzyj.com/cn/article/doi/10.3971/j.issn.1000-8578.2025.24.1326 |
| work_keys_str_mv | AT meileli inhibitoryeffectofexosomesloadedwithginsenosiderh2ongrowthofhepatocellularcarcinomacells AT ziyuezhao inhibitoryeffectofexosomesloadedwithginsenosiderh2ongrowthofhepatocellularcarcinomacells AT kaijin inhibitoryeffectofexosomesloadedwithginsenosiderh2ongrowthofhepatocellularcarcinomacells AT yuanxie inhibitoryeffectofexosomesloadedwithginsenosiderh2ongrowthofhepatocellularcarcinomacells |