Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization Models
2D characterization of organoids by light microscopy with live cell imaging systems provides a powerful, rapid approach toward characterizing organoid growth patterns and behavior under different conditions with high temporal resolution. However, current conventional analysis methods display critica...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-05-01
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| Series: | Advanced NanoBiomed Research |
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| Online Access: | https://doi.org/10.1002/anbr.202400138 |
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| author | Woojin Yang Eva Blahusova Reece McCoy Róisín M. Owens Matthias Zilbauer |
| author_facet | Woojin Yang Eva Blahusova Reece McCoy Róisín M. Owens Matthias Zilbauer |
| author_sort | Woojin Yang |
| collection | DOAJ |
| description | 2D characterization of organoids by light microscopy with live cell imaging systems provides a powerful, rapid approach toward characterizing organoid growth patterns and behavior under different conditions with high temporal resolution. However, current conventional analysis methods display critical flaws in their approximations, including inaccurate assumptions of linear light absorption kinetics and inappropriate normalization of organoid darkness. Organoid darkness represents cellular shedding and debris accumulation in the lumen of organoids and is thus proportional to the surface area, rather than to a 2D organoid projection as is conventionally used. This novel model and image processing pipeline accounts for these shortcomings by incorporating logarithmic light absorption parameters, a noncumulative measure of darkness, and surface area‐normalized darkness values which yield accurate and highly reproducible representation of organoid growth kinetics. |
| format | Article |
| id | doaj-art-9d5041b33ea8411a8f1df590a7e69daa |
| institution | DOAJ |
| issn | 2699-9307 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj-art-9d5041b33ea8411a8f1df590a7e69daa2025-08-20T02:56:09ZengWiley-VCHAdvanced NanoBiomed Research2699-93072025-05-0155n/an/a10.1002/anbr.202400138Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization ModelsWoojin Yang0Eva Blahusova1Reece McCoy2Róisín M. Owens3Matthias Zilbauer4Cambridge Stem Cell Institute University of Cambridge Cambridge CB2 0AW UKCambridge Stem Cell Institute University of Cambridge Cambridge CB2 0AW UKDepartment of Chemical Engineering and Biotechnology University of Cambridge Cambridge CB3 0AS UKDepartment of Chemical Engineering and Biotechnology University of Cambridge Cambridge CB3 0AS UKCambridge Stem Cell Institute University of Cambridge Cambridge CB2 0AW UK2D characterization of organoids by light microscopy with live cell imaging systems provides a powerful, rapid approach toward characterizing organoid growth patterns and behavior under different conditions with high temporal resolution. However, current conventional analysis methods display critical flaws in their approximations, including inaccurate assumptions of linear light absorption kinetics and inappropriate normalization of organoid darkness. Organoid darkness represents cellular shedding and debris accumulation in the lumen of organoids and is thus proportional to the surface area, rather than to a 2D organoid projection as is conventionally used. This novel model and image processing pipeline accounts for these shortcomings by incorporating logarithmic light absorption parameters, a noncumulative measure of darkness, and surface area‐normalized darkness values which yield accurate and highly reproducible representation of organoid growth kinetics.https://doi.org/10.1002/anbr.2024001383D modelinggrowth dynamicslive imagingorganoid darknessorganoids |
| spellingShingle | Woojin Yang Eva Blahusova Reece McCoy Róisín M. Owens Matthias Zilbauer Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization Models Advanced NanoBiomed Research 3D modeling growth dynamics live imaging organoid darkness organoids |
| title | Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization Models |
| title_full | Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization Models |
| title_fullStr | Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization Models |
| title_full_unstemmed | Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization Models |
| title_short | Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization Models |
| title_sort | novel quantitative assessment pipeline of organoid growth dynamics using adapted light absorption and surface area normalization models |
| topic | 3D modeling growth dynamics live imaging organoid darkness organoids |
| url | https://doi.org/10.1002/anbr.202400138 |
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