Development of a Liquid Chromatography–Tandem Mass Spectrometry Method for Oxylipin Analysis and Its Application to Children’s Plasma
<b>Background/Objectives:</b> Oxylipins, a family of oxygenated natural products derived from polyunsaturated fatty acids (PUFAs), play crucial roles in various physiological processes. Evaluating their levels in vivo helps to reveal their roles in health and disease. Because of the nume...
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2025-07-01
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| author | Yonghan Li Siddabasave Gowda B. Gowda Divyavani Gowda Atsuko Ikeda Yu Ait Bamai Rahel Mesfin Ketema Reiko Kishi Hitoshi Chiba Shu-Ping Hui |
| author_facet | Yonghan Li Siddabasave Gowda B. Gowda Divyavani Gowda Atsuko Ikeda Yu Ait Bamai Rahel Mesfin Ketema Reiko Kishi Hitoshi Chiba Shu-Ping Hui |
| author_sort | Yonghan Li |
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| description | <b>Background/Objectives:</b> Oxylipins, a family of oxygenated natural products derived from polyunsaturated fatty acids (PUFAs), play crucial roles in various physiological processes. Evaluating their levels in vivo helps to reveal their roles in health and disease. Because of the numerous isomers of oxylipins, it is essential to develop efficient and precise analytical methods for their identification and quantification. The objective of this study is to establish a quantitative method for oxylipin analysis and its application to the assessment of oxylipins in children’s plasma, with potential implications for diagnostic use in pediatric populations. <b>Methods:</b> A liquid chromatography–electrospray ionization–tandem mass spectrometry method was developed to quantify 64 oxylipins and four precursor PUFAs within 36 min. The limits of quantification ranged from 0.25 to 50 pg, with most analytes showing recoveries and matrix effects between 85 and 110% and between 90 and 110%, respectively. Intra- and inter-day precision values were within 15%. The established method was applied to plasma samples from children aged 9–12 years (boys = 181; girls = 161) in Hokkaido, Japan, to assess the relation between plasma oxylipin and PUFA levels and age, sex, and body mass index. <b>Results:</b> There was no significant correlation between oxylipin levels and age, sex, or body mass index. However, among the PUFAs, boys had higher eicosapentaenoic acid and arachidonic acid levels than those of girls, with a significant increase in eicosapentaenoic acid levels in the overweight group compared with those in the underweight group. <b>Conclusions:</b> We successfully developed a simple and highly selective method for the analysis of oxylipins in preadolescent children’s plasma samples. Thus, this study provides a foundation for broader application of the developed method to different biological samples in future studies. |
| format | Article |
| id | doaj-art-9d4eb06c22494730a84b61b7d84cb402 |
| institution | DOAJ |
| issn | 2075-4418 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Diagnostics |
| spelling | doaj-art-9d4eb06c22494730a84b61b7d84cb4022025-08-20T03:02:58ZengMDPI AGDiagnostics2075-44182025-07-011515187010.3390/diagnostics15151870Development of a Liquid Chromatography–Tandem Mass Spectrometry Method for Oxylipin Analysis and Its Application to Children’s PlasmaYonghan Li0Siddabasave Gowda B. Gowda1Divyavani Gowda2Atsuko Ikeda3Yu Ait Bamai4Rahel Mesfin Ketema5Reiko Kishi6Hitoshi Chiba7Shu-Ping Hui8Graduate School of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanGraduate School of Global Food Resources, Hokkaido University, Kita-9, Nishi-9, Kita-Ku, Sapporo 060-0809, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanCenter for Environmental and Health Sciences, Hokkaido University, Kita-12, Nishi-7, Kita-ku, Sapporo 060-0812, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanCenter for Environmental and Health Sciences, Hokkaido University, Kita-12, Nishi-7, Kita-ku, Sapporo 060-0812, JapanDepartment of Nutrition, Sapporo University of Health Sciences, Nakanuma Nishi-4-2-1-15, Higashi-ku, Sapporo 070-0894, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, Japan<b>Background/Objectives:</b> Oxylipins, a family of oxygenated natural products derived from polyunsaturated fatty acids (PUFAs), play crucial roles in various physiological processes. Evaluating their levels in vivo helps to reveal their roles in health and disease. Because of the numerous isomers of oxylipins, it is essential to develop efficient and precise analytical methods for their identification and quantification. The objective of this study is to establish a quantitative method for oxylipin analysis and its application to the assessment of oxylipins in children’s plasma, with potential implications for diagnostic use in pediatric populations. <b>Methods:</b> A liquid chromatography–electrospray ionization–tandem mass spectrometry method was developed to quantify 64 oxylipins and four precursor PUFAs within 36 min. The limits of quantification ranged from 0.25 to 50 pg, with most analytes showing recoveries and matrix effects between 85 and 110% and between 90 and 110%, respectively. Intra- and inter-day precision values were within 15%. The established method was applied to plasma samples from children aged 9–12 years (boys = 181; girls = 161) in Hokkaido, Japan, to assess the relation between plasma oxylipin and PUFA levels and age, sex, and body mass index. <b>Results:</b> There was no significant correlation between oxylipin levels and age, sex, or body mass index. However, among the PUFAs, boys had higher eicosapentaenoic acid and arachidonic acid levels than those of girls, with a significant increase in eicosapentaenoic acid levels in the overweight group compared with those in the underweight group. <b>Conclusions:</b> We successfully developed a simple and highly selective method for the analysis of oxylipins in preadolescent children’s plasma samples. Thus, this study provides a foundation for broader application of the developed method to different biological samples in future studies.https://www.mdpi.com/2075-4418/15/15/1870oxylipinspolyunsaturated fatty acidsplasmachildrenliquid chromatographymass spectrometry |
| spellingShingle | Yonghan Li Siddabasave Gowda B. Gowda Divyavani Gowda Atsuko Ikeda Yu Ait Bamai Rahel Mesfin Ketema Reiko Kishi Hitoshi Chiba Shu-Ping Hui Development of a Liquid Chromatography–Tandem Mass Spectrometry Method for Oxylipin Analysis and Its Application to Children’s Plasma Diagnostics oxylipins polyunsaturated fatty acids plasma children liquid chromatography mass spectrometry |
| title | Development of a Liquid Chromatography–Tandem Mass Spectrometry Method for Oxylipin Analysis and Its Application to Children’s Plasma |
| title_full | Development of a Liquid Chromatography–Tandem Mass Spectrometry Method for Oxylipin Analysis and Its Application to Children’s Plasma |
| title_fullStr | Development of a Liquid Chromatography–Tandem Mass Spectrometry Method for Oxylipin Analysis and Its Application to Children’s Plasma |
| title_full_unstemmed | Development of a Liquid Chromatography–Tandem Mass Spectrometry Method for Oxylipin Analysis and Its Application to Children’s Plasma |
| title_short | Development of a Liquid Chromatography–Tandem Mass Spectrometry Method for Oxylipin Analysis and Its Application to Children’s Plasma |
| title_sort | development of a liquid chromatography tandem mass spectrometry method for oxylipin analysis and its application to children s plasma |
| topic | oxylipins polyunsaturated fatty acids plasma children liquid chromatography mass spectrometry |
| url | https://www.mdpi.com/2075-4418/15/15/1870 |
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