Ovotransferrin-depleted egg white hydrolysate reduces blood pressure in spontaneously hypertensive rats
Egg whites contain a variety of biologically active proteins and are a good source of bioactive peptides. Egg white hydrolysate prepared by thermoase (EWH) possesses antihypertensive effects. Our recent study showed that egg white proteins, after depleting ovotransferrin, also contained many angiote...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2024-01-01
|
| Series: | Food Materials Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/fmr-0024-0026 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849698480220536832 |
|---|---|
| author | Fatemeh Ashkar Hongbing Fan Zihan Wang Ilekuttige Priyan Shanura Fernando Jianping Wu |
| author_facet | Fatemeh Ashkar Hongbing Fan Zihan Wang Ilekuttige Priyan Shanura Fernando Jianping Wu |
| author_sort | Fatemeh Ashkar |
| collection | DOAJ |
| description | Egg whites contain a variety of biologically active proteins and are a good source of bioactive peptides. Egg white hydrolysate prepared by thermoase (EWH) possesses antihypertensive effects. Our recent study showed that egg white proteins, after depleting ovotransferrin, also contained many angiotensin-converting enzyme (ACE)-inhibitory peptides after hydrolysis using thermoase. In the present study, we aimed to explore the antihypertensive effect of the ovotransferrin-depleted egg white hydrolysate prepared by thermoase (OD-EWH) in spontaneously hypertensive rats, using EWH as the reference. The animals were orally administered with either hydrolysate at a dose of 1 g/kg body weight/d over 9 d. OD-EWH treatment significantly reduced blood pressure to a comparable extent to EWH (p < 0.05). OD-EWH upregulated the aortic expression of angiotensin-converting enzyme 2 and Mas receptor and inhibited the renal ACE (p < 0.05), without affecting the aortic ACE as well as aortic and renal angiotensin II receptors. It also significantly (p < 0.05) enhanced the aortic expression of endothelial nitric oxide synthase and reduced vascular oxidative stress, despite failing to mitigate the inflammation in the aortas. OD-EWH might reduce blood pressure via the modulation of the renin-angiotensin system, endothelial dysfunction, and oxidative stress. These findings demonstrate the potential of OD-EWH as a functional food ingredient against hypertension. |
| format | Article |
| id | doaj-art-9d4d3f1adaff4fa491b7101650691405 |
| institution | DOAJ |
| issn | 2771-4683 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Food Materials Research |
| spelling | doaj-art-9d4d3f1adaff4fa491b71016506914052025-08-20T03:18:54ZengMaximum Academic PressFood Materials Research2771-46832024-01-01411810.48130/fmr-0024-0026fmr-0024-0026Ovotransferrin-depleted egg white hydrolysate reduces blood pressure in spontaneously hypertensive ratsFatemeh Ashkar0Hongbing Fan1Zihan Wang2Ilekuttige Priyan Shanura Fernando3Jianping Wu4Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, T6G 2P5, AB, CanadaDepartment of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, T6G 2P5, AB, CanadaDepartment of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, T6G 2P5, AB, CanadaDepartment of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, T6G 2P5, AB, CanadaDepartment of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, T6G 2P5, AB, CanadaEgg whites contain a variety of biologically active proteins and are a good source of bioactive peptides. Egg white hydrolysate prepared by thermoase (EWH) possesses antihypertensive effects. Our recent study showed that egg white proteins, after depleting ovotransferrin, also contained many angiotensin-converting enzyme (ACE)-inhibitory peptides after hydrolysis using thermoase. In the present study, we aimed to explore the antihypertensive effect of the ovotransferrin-depleted egg white hydrolysate prepared by thermoase (OD-EWH) in spontaneously hypertensive rats, using EWH as the reference. The animals were orally administered with either hydrolysate at a dose of 1 g/kg body weight/d over 9 d. OD-EWH treatment significantly reduced blood pressure to a comparable extent to EWH (p < 0.05). OD-EWH upregulated the aortic expression of angiotensin-converting enzyme 2 and Mas receptor and inhibited the renal ACE (p < 0.05), without affecting the aortic ACE as well as aortic and renal angiotensin II receptors. It also significantly (p < 0.05) enhanced the aortic expression of endothelial nitric oxide synthase and reduced vascular oxidative stress, despite failing to mitigate the inflammation in the aortas. OD-EWH might reduce blood pressure via the modulation of the renin-angiotensin system, endothelial dysfunction, and oxidative stress. These findings demonstrate the potential of OD-EWH as a functional food ingredient against hypertension.https://www.maxapress.com/article/doi/10.48130/fmr-0024-0026ovotransferrin-depleted egg whiteblood pressure reductionrenin-angiotensin systeminflammationoxidative stress |
| spellingShingle | Fatemeh Ashkar Hongbing Fan Zihan Wang Ilekuttige Priyan Shanura Fernando Jianping Wu Ovotransferrin-depleted egg white hydrolysate reduces blood pressure in spontaneously hypertensive rats Food Materials Research ovotransferrin-depleted egg white blood pressure reduction renin-angiotensin system inflammation oxidative stress |
| title | Ovotransferrin-depleted egg white hydrolysate reduces blood pressure in spontaneously hypertensive rats |
| title_full | Ovotransferrin-depleted egg white hydrolysate reduces blood pressure in spontaneously hypertensive rats |
| title_fullStr | Ovotransferrin-depleted egg white hydrolysate reduces blood pressure in spontaneously hypertensive rats |
| title_full_unstemmed | Ovotransferrin-depleted egg white hydrolysate reduces blood pressure in spontaneously hypertensive rats |
| title_short | Ovotransferrin-depleted egg white hydrolysate reduces blood pressure in spontaneously hypertensive rats |
| title_sort | ovotransferrin depleted egg white hydrolysate reduces blood pressure in spontaneously hypertensive rats |
| topic | ovotransferrin-depleted egg white blood pressure reduction renin-angiotensin system inflammation oxidative stress |
| url | https://www.maxapress.com/article/doi/10.48130/fmr-0024-0026 |
| work_keys_str_mv | AT fatemehashkar ovotransferrindepletedeggwhitehydrolysatereducesbloodpressureinspontaneouslyhypertensiverats AT hongbingfan ovotransferrindepletedeggwhitehydrolysatereducesbloodpressureinspontaneouslyhypertensiverats AT zihanwang ovotransferrindepletedeggwhitehydrolysatereducesbloodpressureinspontaneouslyhypertensiverats AT ilekuttigepriyanshanurafernando ovotransferrindepletedeggwhitehydrolysatereducesbloodpressureinspontaneouslyhypertensiverats AT jianpingwu ovotransferrindepletedeggwhitehydrolysatereducesbloodpressureinspontaneouslyhypertensiverats |